{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T22:58:07Z","timestamp":1787957887026,"version":"build-2784847793"},"reference-count":16,"publisher":"Microbiology Society","issue":"Pt_11","funder":[{"name":"FOU","award":["VGFOUREG 30781"],"award-info":[{"award-number":["VGFOUREG 30781"]}]},{"name":"FOU","award":["VGFOUREG-72241"],"award-info":[{"award-number":["VGFOUREG-72241"]}]},{"name":"ALF-medel","award":["ALFGBG-11574"],"award-info":[{"award-number":["ALFGBG-11574"]}]},{"name":"Swedish Research Council-Medicine and Health"},{"name":"Swedish Research Council-Natural and Engineering Sciences"},{"name":"European Union\u2019s Seventh Framework Programme","award":["222720"],"award-info":[{"award-number":["222720"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,11,1]]},"abstract":"Two novel obligately anaerobic, Gram-stain-positive, saccharolytic and non-proteolytic spore-forming bacilli (strains CD3\u200a:\u200a22T<\/jats:sup> and N1T<\/jats:sup>) are described. Strain CD3\u200a:\u200a22T<\/jats:sup> was isolated from a biopsy of the small intestine of a child with coeliac disease, and strain N1T<\/jats:sup> from the saliva of a healthy young man. The cells of both strains were observed to be filamentous, approximately 5 to >20 \u00b5m long, some of them curving and with swellings. The novel organisms produced H2<\/jats:sub>S, NH3<\/jats:sub>, butyric acid and acetic acid as major metabolic end products. Phylogenetic analyses, based on comparative 16S rRNA gene sequencing, revealed close relationships (98\u200a% sequence similarity) between the two isolates, as well as the type strain of \n \n Eubacterium saburreum<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> and four other \n \n Lachnospiraceae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> bacterium-\/\n \n E. saburreum<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>-like organisms. This group of bacteria were clearly different from any of the 19 known genera in the family \n \n Lachnospiraceae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>. While \n \n Eubacterium<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> species are reported to be non-spore-forming, reanalysis of \n \n E. saburreum<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> CCUG 28089T<\/jats:sup> confirmed that the bacterium is indeed able to form spores. Based on 16S rRNA gene sequencing, phenotypic and biochemical properties, strains CD3\u200a:\u200a22T<\/jats:sup> and N1T<\/jats:sup> represent novel species of a new and distinct genus, named Lachnoanaerobaculum<\/jats:italic> gen. nov., in the family \n \n Lachnospiraceae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> [within the order \n \n Clostridiales<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>, class \n \n Clostridia<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>, phylum \n \n Firmicutes<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>]. Strain CD3\u200a:\u200a22T<\/jats:sup> (\u200a=\u200aCCUG 58757T<\/jats:sup> \u200a=\u200aDSM 23576T<\/jats:sup>) is the type strain of the type species, Lachnoanaerobaculum umeaense<\/jats:italic> gen. nov., sp. nov., of the proposed new genus. Strain N1T<\/jats:sup> (\u200a=\u200aCCUG 60305T<\/jats:sup>\u200a=\u200aDSM 24553T<\/jats:sup>) is the type strain of Lachnoanaerobaculum orale<\/jats:italic> sp. nov. Moreover, \n \n Eubacterium saburreum<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> is reclassified as Lachnoanaerobaculum saburreum<\/jats:italic> comb. nov. (type strain CCUG 28089T<\/jats:sup> \u200a=\u200aATCC 33271T<\/jats:sup> \u200a=\u200aCIP 105341T<\/jats:sup> \u200a=\u200aDSM 3986T<\/jats:sup> \u200a=\u200aJCM 11021T<\/jats:sup> \u200a=\u200aVPI 11763T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.033613-0","type":"journal-article","created":{"date-parts":[[2012,1,7]],"date-time":"2012-01-07T04:46:03Z","timestamp":1325911563000},"page":"2685-2690","source":"Crossref","is-referenced-by-count":77,"title":["Lachnoanaerobaculum gen. nov., a new genus in the \n \n Lachnospiraceae\n \n : characterization of Lachnoanaerobaculum umeaense gen. nov., sp. nov., isolated from the human small intestine, and Lachnoanaerobaculum orale sp. nov., isolated from saliva, and reclassification of \n \n Eubacterium saburreum\n \n (Pr\u00e9vot 1966) Holdeman and Moore 1970 as Lachnoanaerobaculum saburreum comb. nov."],"prefix":"10.1099","volume":"62","author":[{"given":"Maria E.","family":"Hedberg","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Immunology, Ume\u00e5 University, Ume\u00e5 SE-901 87, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward R. B.","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, Sahlgrenska Academy of the University of Gothenburg, G\u00f6teborg SE-405 30, Sweden"},{"name":"CCUG \u2013 Culture Collection University of Gothenburg, Department of Clinical Bacteriology, Sahlgrenska University Hospital, G\u00f6teborg SE-413 45, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liselott","family":"Svensson-Stadler","sequence":"additional","affiliation":[{"name":"CCUG \u2013 Culture Collection University of Gothenburg, Department of Clinical Bacteriology, Sahlgrenska University Hospital, G\u00f6teborg SE-413 45, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per","family":"H\u00f6rstedt","sequence":"additional","affiliation":[{"name":"Department of Medical Biosciences, Pathology, Ume\u00e5 University, Ume\u00e5 SE-901 87, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Baranov","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Clinical Immunology, Ume\u00e5 University, Ume\u00e5 SE-901 87, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olle","family":"Hernell","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Pediatrics, Ume\u00e5 University, Ume\u00e5 SE-901 87, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Nyunt","family":"Wai","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Ume\u00e5 University, Ume\u00e5 SE-901 87, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sten","family":"Hammarstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Immunology, Ume\u00e5 University, Ume\u00e5 SE-901 87, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Louise","family":"Hammarstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Immunology, Ume\u00e5 University, Ume\u00e5 SE-901 87, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-44-4-812"},{"key":"r2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00542-10"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2004.04157.x"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01206-08"},{"key":"r5","first-page":"23","article-title":"Eubacterium","volume-title":"In Outline of Clinical Methods in Anaerobic Bacteriology","author":"Holdeman","year":"1970"},{"key":"r6","volume-title":"Anaerobe Laboratory Manual","author":"Holdeman","year":"1977"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2000.tb01210.x"},{"key":"r8","volume-title":"Wadsworth-KTL Anaerobic Bacteriology Manual","author":"Jousimies-Somer","year":"2002"},{"key":"r9","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2009.524"},{"key":"r10","volume-title":"Manual for the Classification and Determination of the Anaerobic Bacteria","author":"Pr\u00e9vot","year":"1966"},{"key":"r11","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"r12","article-title":"Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101","year":"2001"},{"key":"r13","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp967"},{"key":"r14","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-44-4-846"},{"key":"r15","doi-asserted-by":"publisher","DOI":"10.1016\/S1075-9964(03)00109-4"},{"key":"r16","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2008.07.002"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijs.0.033613-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,10]],"date-time":"2020-09-10T17:41:40Z","timestamp":1599759700000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijs.0.033613-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,1]]},"references-count":16,"journal-issue":{"issue":"Pt_11","published-print":{"date-parts":[[2012,11,1]]}},"URL":"https:\/\/doi.org\/10.1099\/ijs.0.033613-0","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,1]]}}}