{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:01:28Z","timestamp":1791860488047,"version":"4.3.4"},"reference-count":30,"publisher":"Microbiology Society","issue":"Pt_8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,8,1]]},"abstract":"\n During attempts to obtain novel, human-associated species of the domain\n \n \n Archaea<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n , a coccoid micro-organism, designated strain B10\n T<\/jats:sup>\n , was isolated in pure culture from a sample of human faeces collected in Marseille, France. On the basis of its phenotypic characteristics and 16S rRNA and\n mcr<\/jats:italic>\n A gene sequences, the novel strain was classified as a methanogenic archaeon. Cells of the strain were non-motile, Gram-staining-positive cocci that were approximately 850 nm in diameter and showed autofluorescence at 420 nm. Cells were lysed by 0.1\u200a% (w\/v) SDS. With hydrogen as the electron donor, strain B10\n T<\/jats:sup>\n produced methane by reducing methanol. The novel strain was unable to produce methane when hydrogen or methanol was the sole energy source. In an atmosphere containing CO\n 2<\/jats:sub>\n , strain B10\n T<\/jats:sup>\n could not produce methane from formate, acetate, trimethylamine, 2-butanol, 2-propanol, cyclopentanol, 2-pentanol, ethanol, 1-propanol or 2,3-butanediol. Strain B10\n T<\/jats:sup>\n grew optimally with 0.5\u20131.0\u200a% (w\/v) NaCl, at pH 7.6 and at 37 \u00b0C. It required tungstate-selenite for growth. The complete genome of the novel strain was sequenced; the size of the genome was estimated to be 2.05 Mb and the genomic DNA G+C content was 59.93 mol%. In phylogenetic analyses based on 16S rRNA gene sequences, the highest sequence similarities (98.0\u201398.7\u200a%) were seen between strain B10\n T<\/jats:sup>\n and several uncultured, methanogenic\n \n \n Archaea<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n that had been collected from the digestive tracts of a cockroach, a chicken and mammals. In the same analysis, the non-methanogenic \u2018\n Candidatus<\/jats:italic>\n Aciduliprofundum boonei\u2019 DSM 19572 was identified as the cultured micro-organism that was most closely related to strain B10\n T<\/jats:sup>\n (83.0\u200a% 16S rRNA gene sequence similarity). Each of the three treeing algorithms used in the analysis of 16S rRNA gene sequences indicated that strain B10\n T<\/jats:sup>\n belongs to a novel order that is distinct from the\n \n \n Thermoplasmatales<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n . The novel strain also appeared to be distinct from\n \n \n Methanosphaera stadtmanae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n DSM 3091\n T<\/jats:sup>\n (72.9\u200a% 16S rRNA gene sequence similarity), another methanogenic archaeon that was isolated from human faeces and can use methanol in the presence of hydrogen. Based on the genetic and phenotypic evidence, strain B10\n T<\/jats:sup>\n represents a novel species of a new genus for which the name\n Methanomassiliicoccus luminyensis<\/jats:italic>\n gen. nov., sp. nov. is proposed. The type strain of the type species is B10\n T<\/jats:sup>\n (\u200a=\u200aDSM 24529\n T<\/jats:sup>\n \u200a=\u200aCSUR P135\n T<\/jats:sup>\n ).\n <\/jats:p>","DOI":"10.1099\/ijs.0.033712-0","type":"journal-article","created":{"date-parts":[[2012,8,2]],"date-time":"2012-08-02T19:43:23Z","timestamp":1343936603000},"page":"1902-1907","source":"Crossref","is-referenced-by-count":561,"title":["Methanomassiliicoccus luminyensis gen. nov., sp. nov., a methanogenic archaeon isolated from human faeces"],"prefix":"10.1099","volume":"62","author":[{"given":"B\u00e9dis","family":"Dridi","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS 6236 IDR 198, Facult\u00e9 de M\u00e9decine, IFR48, Aix-Marseille-Universit\u00e9, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Laure","family":"Fardeau","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie IRD, UMR D180, Microbiologie et Biotechnologie des Environnements Chauds, Aix-Provence Universit\u00e9, \u00c9cole Sup\u00e9rieure d'Ing\u00e9nieurs de Luminy, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Ollivier","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie IRD, UMR D180, Microbiologie et Biotechnologie des Environnements Chauds, Aix-Provence Universit\u00e9, \u00c9cole Sup\u00e9rieure d'Ing\u00e9nieurs de Luminy, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS 6236 IDR 198, Facult\u00e9 de M\u00e9decine, IFR48, Aix-Marseille-Universit\u00e9, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Drancourt","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS 6236 IDR 198, Facult\u00e9 de M\u00e9decine, IFR48, Aix-Marseille-Universit\u00e9, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1128\/MMBR.43.2.260-296.1979","article-title":"Methanogens: reevaluation of a unique biological group","volume":"43","author":"Balch","year":"1979","journal-title":"Microbiol Rev"},{"key":"r2","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1128\/AEM.52.5.1080-1085.1986","article-title":"Relationship of formate to growth and methanogenesis by Methanococcus thermolithotrophicus","volume":"52","author":"Belay","year":"1986","journal-title":"Appl Environ Microbiol"},{"key":"r3","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-38-2-212"},{"key":"r4","doi-asserted-by":"publisher","DOI":"10.1007\/BF01568654"},{"key":"r5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007063"},{"key":"r6","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02436-08"},{"key":"r7","doi-asserted-by":"publisher","DOI":"10.1007\/BF01570184"},{"key":"r8","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.9.5483-5491.2003"},{"key":"r9","first-page":"123","article-title":"The roll-tube method for cultivation of strict anaerobes","volume":"17","author":"Hungate","year":"1973","journal-title":"Bull Ecol Res Comm"},{"key":"r10","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1128\/JB.130.3.1404-1406.1977","article-title":"Methanococcus vannielii: culture and effects of selenium and tungsten on growth","volume":"130","author":"Jones","year":"1977","journal-title":"J Bacteriol"},{"key":"r11","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/S0021-9258(19)70024-6","article-title":"Selenium-dependent and selenium-independent formate dehydrogenases of Methanococcus vannielii. 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