{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:42:29Z","timestamp":1791261749248,"version":"4.1.0"},"reference-count":31,"publisher":"Microbiology Society","issue":"Pt_7","funder":[{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Region Auvergne"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,7,1]]},"abstract":"<jats:p>An autotrophic, hydrogenotrophic methanogen, designated strain 17A1<jats:sup>T<\/jats:sup>, was isolated from the profundal sediment of the meromictic Lake Pavin, France. The cells of the novel strain, which were non-motile, Gram-staining-negative rods that measured 2\u201315 \u00b5m in length and 0.2\u20130.4 \u00b5m in width, grew as filaments. Strain 17A1<jats:sup>T<\/jats:sup> grew in a mineral medium and its growth was stimulated by the addition of yeast extract, vitamins, acetate or rumen fluid. Penicillin, vancomycin and kanamycin reduced growth but did not completely inhibit it. Growth occurred at 14\u201341 \u00b0C (optimum 30 \u00b0C), at pH 5.0\u20138.5 (optimum pH 6.5) and with 0\u20130.4 M NaCl (optimum 0.1 M). The novel strain utilized H<jats:sub>2<\/jats:sub>\/CO<jats:sub>2<\/jats:sub> and methanol\/H<jats:sub>2<\/jats:sub> as substrates but not formate, acetate, methylamine\/H<jats:sub>2<\/jats:sub>, isobutanol or 2-propanol. Its genomic DNA G+C content was 37.0 mol%. In phylogenetic analyses based on 16S rRNA gene sequences, strain 17A1<jats:sup>T<\/jats:sup> appeared to be a member of the genus <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus\" xlink:type=\"simple\">\n                     <jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.94\" xlink:type=\"simple\">\n                        <jats:italic>Methanobacterium<\/jats:italic>\n                     <\/jats:ext-link>\n                  <\/jats:named-content>, with <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"species\" xlink:type=\"simple\">\n                     <jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.8696\" xlink:type=\"simple\">\n                        <jats:italic>Methanobacterium beijingense<\/jats:italic>\n                     <\/jats:ext-link>\n                  <\/jats:named-content> 8-2<jats:sup>T<\/jats:sup> (96.3\u200a% sequence similarity) identified as the most closely related established species. Based on phenotypic and phylogenetic data, strain 17A1<jats:sup>T<\/jats:sup> represents a novel species of methanogen within the genus <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus\" xlink:type=\"simple\">\n                     <jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.94\" xlink:type=\"simple\">\n                        <jats:italic>Methanobacterium<\/jats:italic>\n                     <\/jats:ext-link>\n                  <\/jats:named-content>, for which the name <jats:italic>Methanobacterium lacus<\/jats:italic> sp. nov. is proposed. The type strain is 17A1<jats:sup>T<\/jats:sup> (\u200a=\u200aDSM 24406<jats:sup>T<\/jats:sup>\u200a=\u200aJCM 17760<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.034538-0","type":"journal-article","created":{"date-parts":[[2011,9,3]],"date-time":"2011-09-03T01:07:19Z","timestamp":1315012039000},"page":"1625-1629","source":"Crossref","is-referenced-by-count":56,"title":["Methanobacterium lacus sp. nov., isolated from the profundal sediment of a freshwater meromictic lake"],"prefix":"10.1099","volume":"62","author":[{"given":"Guillaume","family":"Borrel","sequence":"first","affiliation":[{"name":"UMR CNRS 6023, Universit\u00e9 Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France"},{"name":"Clermont Universit\u00e9, Universit\u00e9 Blaise Pascal, Laboratoire Microorganismes: G\u00e9nome et Environnement, BP 10448, F-63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith","family":"Joblin","sequence":"additional","affiliation":[{"name":"UMR CNRS 6023, Universit\u00e9 Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France"},{"name":"Clermont Universit\u00e9, Universit\u00e9 Blaise Pascal, Laboratoire Microorganismes: G\u00e9nome et Environnement, BP 10448, F-63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie","family":"Guedon","sequence":"additional","affiliation":[{"name":"UMR CNRS 6023, Universit\u00e9 Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France"},{"name":"Clermont Universit\u00e9, Universit\u00e9 Blaise Pascal, Laboratoire Microorganismes: G\u00e9nome et Environnement, BP 10448, F-63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Colombet","sequence":"additional","affiliation":[{"name":"UMR CNRS 6023, Universit\u00e9 Blaise Pascal, BP 10448, 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