{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T02:57:04Z","timestamp":1778900224248,"version":"3.51.4"},"reference-count":28,"publisher":"Microbiology Society","issue":"Pt_12","funder":[{"DOI":"10.13039\/501100006447","name":"University of Zurich","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006447","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,12,1]]},"abstract":"<jats:p>Four Gram-stain-positive, catalase-negative, coccus-shaped bacterial strains were isolated from multiple blood cultures of patients with endocarditis, meningitis and spondylodiscitis. The isolates were tentatively identified as viridans streptococci on the basis of phenotypic characteristics. Comparative 16S rRNA gene sequencing studies showed that the organisms were members of the<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"species\" xlink:type=\"simple\"><jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.5661\" xlink:type=\"simple\"><jats:italic>Streptococcus mitis<\/jats:italic><\/jats:ext-link><\/jats:named-content>group but did not correspond to any recognized species. The nearest phylogenetic relative was<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"species\" xlink:type=\"simple\"><jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.5661\" xlink:type=\"simple\"><jats:italic>S. mitis<\/jats:italic><\/jats:ext-link><\/jats:named-content>ATCC 49456<jats:sup>T<\/jats:sup>, with 98.6\u200a% sequence similarity. The representative strain AZ_3a<jats:sup>T<\/jats:sup>showed less than 96.8, 97.6, 94.5 and 95.5\u200a% similarity to the phylogenetically most closely related species by<jats:italic>recA<\/jats:italic>,<jats:italic>rpoB<\/jats:italic>,<jats:italic>sodA<\/jats:italic>and<jats:italic>groEL<\/jats:italic>gene sequence analysis, respectively. DNA\u2013DNA hybridization analyses showed a low reassociation value of 32.2\u200a% between strain AZ_3a<jats:sup>T<\/jats:sup>and<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"species\" xlink:type=\"simple\"><jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.5661\" xlink:type=\"simple\"><jats:italic>S. mitis<\/jats:italic><\/jats:ext-link><\/jats:named-content>DSM 12643<jats:sup>T<\/jats:sup>. Reassociation values with members of other<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"species\" xlink:type=\"simple\"><jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.5661\" xlink:type=\"simple\"><jats:italic>S. mitis<\/jats:italic><\/jats:ext-link><\/jats:named-content>group species ranged from 27.3 to 49.7\u200a%. The G+C content of the DNA was 40.0 mol%. Based on our biochemical and molecular analyses, the isolates represent a novel species, for which the name<jats:italic>Streptococcus<\/jats:italic><jats:italic>tigurinus<\/jats:italic>sp. nov. is proposed. The type strain is AZ_3a<jats:sup>T<\/jats:sup>(\u200a=\u200aCCOS 600<jats:sup>T<\/jats:sup>\u200a=\u200aDSM 24864<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.038299-0","type":"journal-article","created":{"date-parts":[[2012,2,22]],"date-time":"2012-02-22T07:07:34Z","timestamp":1329894454000},"page":"2941-2945","source":"Crossref","is-referenced-by-count":62,"title":["Streptococcus tigurinus sp. nov., isolated from blood of patients with endocarditis, meningitis and spondylodiscitis"],"prefix":"10.1099","volume":"62","author":[{"given":"Andrea","family":"Zbinden","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, University of Zurich, Gloriastrasse 30\/32, CH-8006 Z\u00fcrich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas J.","family":"Mueller","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, R\u00e4mistrasse 100, CH-8091 Z\u00fcrich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip E.","family":"Tarr","sequence":"additional","affiliation":[{"name":"Infectious Diseases Service, Kantonsspital Bruderholz, University of Basel, Petersplatz 1, CH-4003 Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cathrin","family":"Spr\u00f6er","sequence":"additional","affiliation":[{"name":"DSMZ \u2013 Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstra\u00dfe 7B, D-38124 Braunschweig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter M.","family":"Keller","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University of Zurich, Gloriastrasse 30\/32, CH-8006 Z\u00fcrich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido V.","family":"Bloemberg","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University of 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