{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T19:16:06Z","timestamp":1793474166472,"version":"4.5.0"},"reference-count":27,"publisher":"Microbiology Society","issue":"Pt_2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,2,1]]},"abstract":"A thorough phenotypic and genotypic analysis of 150 strains belonging to the \n \n Mycobacterium terrae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> complex resulted in the identification of a number of previously unreported sequevars (sqvs) within the species known to belong to the complex. For the species \n \n Mycobacterium arupense<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>, three sqvs were detected in the 16S rRNA gene, six sqvs in the hsp65<\/jats:italic> gene and 15 sqvs in the rpoB<\/jats:italic> gene; in \n \n Mycobacterium senuense<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> two sqvs were present in each of the three genetic regions; in \n \n Mycobacterium kumamotonense<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> four, two and nine sqvs were found, respectively, and in \n \n M. terrae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> three, four and six sqvs were found, respectively. The inappropriate inclusion of \n \n Mycobacterium triviale<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> within the \n \n M. terrae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> complex was confirmed. The limited utility of biochemical tests and of mycolic acid analyses for the differentiation of the members of \n \n M. terrae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> complex was also confirmed. The survey allowed the recognition of three previously undescribed species that were characterized by unique sequences in the 16S rRNA, hsp65<\/jats:italic> and rpoB<\/jats:italic> genes. Mycobacterium engbaekii<\/jats:italic> sp. nov. (proposed previously 40 years ago but never validly published) was characterized by pink photochromogenic pigmentation and rapid growth; phylogenetically it was related to \n \n Mycobacterium hiberniae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>. The type strain of this species, of which eight strains were investigated, is ATCC 27353T<\/jats:sup> (\u200a=\u200aDSM 45694T<\/jats:sup>). A cluster of 24 strains was the basis for the description of Mycobacterium heraklionense<\/jats:italic> sp. nov., which has an intermediate growth rate and is unpigmented; nitrate reductase activity is typically strong. Closely related to \n \n M. arupense<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> with respect to the 16S rRNA gene, M. heraklionense<\/jats:italic> sp. nov. could be clearly differentiated from the latter species in the other genetic regions investigated. The type strain is NCTC 13432T<\/jats:sup> (\u200a=\u200aLMG 24735T<\/jats:sup>\u200a=\u200aCECT 7509T<\/jats:sup>). Mycobacterium longobardum<\/jats:italic> sp. nov., represented in the study by seven strains, was characterized by a unique phylogenetic location within the \n \n M. terrae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> complex, clearly divergent from any other species. The type strain is DSM 45394T<\/jats:sup> (\u200a=\u200aCCUG 58460T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.038737-0","type":"journal-article","created":{"date-parts":[[2012,3,24]],"date-time":"2012-03-24T07:42:06Z","timestamp":1332574926000},"page":"401-411","source":"Crossref","is-referenced-by-count":72,"title":["Survey of 150 strains belonging to the \n \n Mycobacterium terrae\n \n complex and description of Mycobacterium engbaekii sp. nov., Mycobacterium heraklionense sp. nov. and Mycobacterium longobardum sp. nov."],"prefix":"10.1099","volume":"63","author":[{"given":"Enrico","family":"Tortoli","sequence":"first","affiliation":[{"name":"Emerging Bacterial Pathogens Unit, San Raffaele Scientific Institute, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe","family":"Gitti","sequence":"additional","affiliation":[{"name":"Department of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine, University Hospital of Heraklion, Heraklion, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Peter","family":"Klenk","sequence":"additional","affiliation":[{"name":"DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Lauria","sequence":"additional","affiliation":[{"name":"Microbiology and Virology Laboratory, Careggi University Hospital, Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Mannino","sequence":"additional","affiliation":[{"name":"Microbiology and Virology Laboratory, Careggi University Hospital, Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Mantegani","sequence":"additional","affiliation":[{"name":"Emerging Bacterial Pathogens Unit, San Raffaele Scientific Institute, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Mariottini","sequence":"additional","affiliation":[{"name":"Cytogenetics and Genetics Unit, Careggi University Hospital, Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Neonakis","sequence":"additional","affiliation":[{"name":"Department of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine, University Hospital of Heraklion, Heraklion, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1099\/ijs.0.63094-0","article-title":"Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing","volume":"54","author":"Ad\u00e9kambi","year":"2004","journal-title":"Int J Syst Evol Microbiol"},{"key":"r2","doi-asserted-by":"crossref","first-page":"5699","DOI":"10.1128\/JCM.41.12.5699-5708.2003","article-title":"rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria","volume":"41","author":"Ad\u00e9kambi","year":"2003","journal-title":"J Clin Microbiol"},{"key":"r3"},{"key":"r4","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1099\/ijs.0.64194-0","article-title":"Mycobacterium arupense sp. nov., a non-chromogenic bacterium isolated from clinical specimens","volume":"56","author":"Cloud","year":"2006","journal-title":"Int J Syst Evol Microbiol"},{"key":"r5","year":"2011","journal-title":"Susceptibility Testing of Mycobacteria, Nocardiae and Other Aerobic Actinomycetes; Approved Standard"},{"key":"r6","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1099\/ijs.0.63222-0","article-title":"A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model","volume":"55","author":"Devulder","year":"2005","journal-title":"Int J Syst Evol Microbiol"},{"key":"r7","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1099\/00207713-43-2-352","article-title":"Mycobacterium hiberniae sp. nov","volume":"43","author":"Kazda","year":"1993","journal-title":"Int J Syst Bacteriol"},{"key":"r8","volume-title":"Public Health Mycobacteriology. 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