{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:44:57Z","timestamp":1789429497302,"version":"build-2803163510"},"reference-count":26,"publisher":"Microbiology Society","issue":"Pt_5","funder":[{"name":"RSC","award":["14. 740. 11. 0111"],"award-info":[{"award-number":["14. 740. 11. 0111"]}]},{"name":"Russian Foundation for Basic Research","award":["10-04-00808"],"award-info":[{"award-number":["10-04-00808"]}]},{"name":"RSC","award":["14. 740. 11. 0111"],"award-info":[{"award-number":["14. 740. 11. 0111"]}]},{"name":"Russian Foundation for Basic Research","award":["10-04-00808"],"award-info":[{"award-number":["10-04-00808"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,5,1]]},"abstract":"<jats:p>A newly isolated, facultatively methylotrophic bacterium (strain MUSA<jats:sup>T<\/jats:sup>) was investigated. The isolate was strictly aerobic, Gram-stain-negative, asporogenous, motile, rod-shaped and multiplied by binary fission. The strain utilized methanol, methylamine and an apparently narrow range of multi-carbon compounds, but not methane, dichloromethane or CO<jats:sub>2<\/jats:sub>\/H<jats:sub>2<\/jats:sub>, as the carbon and energy sources. Growth occurred at pH 5.5\u20139.5 (optimum, pH 7.0) and 16\u201340 \u00b0C (optimum, 28\u201330 \u00b0C). The major fatty acids of methanol-grown cells were C<jats:sub>18\u200a:\u200a1<\/jats:sub>\u03c97<jats:italic>c<\/jats:italic>, C<jats:sub>18\u200a:\u200a0<\/jats:sub>and 11-methyl-C<jats:sub>18\u200a:\u200a1<\/jats:sub>\u03c97<jats:italic>c<\/jats:italic>. The predominant phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine. The major ubiquinone was Q-10. The strain had methanol and methylamine dehydrogenases as well as the enzymes of the<jats:italic>N<\/jats:italic>-methylglutamate pathway (lyases of \u03b3-glutamylmethylamide and<jats:italic>N<\/jats:italic>-methylglutamate). C<jats:sub>1<\/jats:sub>assimilation occurs via the isocitrate lyase-negative serine pathway. Ammonium was assimilated by glutamate dehydrogenase and the glutamate cycle (glutamate synthase\/glutamine synthetase). The DNA G+C content of the strain was 64.5 mol% (determined from the melting temperature). Based on 16S rRNA gene sequence similarity (97.0\u201398.9\u200a%) and DNA\u2013DNA relatedness (36\u201338\u200a%) with representatives of the genus<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus\" xlink:type=\"simple\"><jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.1434\" xlink:type=\"simple\"><jats:italic>Methylopila<\/jats:italic><\/jats:ext-link><\/jats:named-content>(<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"species\" xlink:type=\"simple\"><jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.1435\" xlink:type=\"simple\"><jats:italic>Methylopila capsulata<\/jats:italic><\/jats:ext-link><\/jats:named-content>IM1<jats:sup>T<\/jats:sup>and<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"species\" xlink:type=\"simple\"><jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.22161\" xlink:type=\"simple\"><jats:italic>Methylopila jiangsuensis<\/jats:italic><\/jats:ext-link><\/jats:named-content>JZL-4<jats:sup>T<\/jats:sup>) the isolate was classified as a novel species of the genus<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus\" xlink:type=\"simple\"><jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.1434\" xlink:type=\"simple\"><jats:italic>Methylopila<\/jats:italic><\/jats:ext-link><\/jats:named-content>, for which the name<jats:italic>Methylopila musalis<\/jats:italic>sp. nov. is proposed. The type strain is MUSA<jats:sup>T<\/jats:sup>(\u200a=\u200aVKM B-2646<jats:sup>T<\/jats:sup>\u200a=\u200aDSM 24986<jats:sup>T<\/jats:sup>\u200a=\u200aCCUG 61696<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.042028-0","type":"journal-article","created":{"date-parts":[[2012,9,15]],"date-time":"2012-09-15T10:28:04Z","timestamp":1347704884000},"page":"1847-1852","source":"Crossref","is-referenced-by-count":17,"title":["Methylopila musalis sp. nov., an aerobic, facultatively methylotrophic bacterium isolated from banana fruit"],"prefix":"10.1099","volume":"63","author":[{"given":"Nina V.","family":"Doronina","sequence":"first","affiliation":[{"name":"G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences and Pushchino State Institute of Natural Sciences, Pushchino, Moscow region 142290, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena N.","family":"Kaparullina","sequence":"additional","affiliation":[{"name":"G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences and Pushchino State Institute of Natural Sciences, Pushchino, Moscow region 142290, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatjana V.","family":"Bykova","sequence":"additional","affiliation":[{"name":"G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences and Pushchino State Institute of Natural Sciences, Pushchino, Moscow region 142290, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri A.","family":"Trotsenko","sequence":"additional","affiliation":[{"name":"G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences and Pushchino State Institute of Natural Sciences, Pushchino, Moscow region 142290, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/S1570-9639(03)00042-6","article-title":"The structure and mechanism of methanol dehydrogenase","volume":"1647","author":"Anthony","year":"2003","journal-title":"Biochim Biophys Acta"},{"key":"r2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/j.1432-1033.1970.tb00830.x","article-title":"The quantitative measurement of DNA hybridization from renaturation rates","volume":"12","author":"De Ley","year":"1970","journal-title":"Eur J Biochem"},{"key":"r4","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/S0723-2020(11)80454-6","article-title":"Isolation and characterization of a new facultatively methylotrophic bacterium: description of Methylorhabdus multivorans gen. nov., sp. nov.","volume":"18","author":"Doronina","year":"1995","journal-title":"Syst Appl Microbiol"},{"key":"r5","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1099\/00207713-48-4-1313","article-title":"Methylopila capsulata gen. nov., sp. nov., a novel non-pigmented aerobic facultatively methylotrophic bacterium","volume":"48","author":"Doronina","year":"1998","journal-title":"Int J Syst Bacteriol"},{"key":"r6","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/S0723-2020(00)80007-7","article-title":"Methylopila helvetica sp. nov. and Methylobacterium dichloromethanicum sp. nov.\u2013novel aerobic facultatively methylotrophic bacteria utilizing dichloromethane","volume":"23","author":"Doronina","year":"2000","journal-title":"Syst Appl Microbiol"},{"key":"r7","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1099\/00207713-51-3-1051","article-title":"Albibacter methylovorans gen. nov., sp. nov., a novel aerobic, facultatively autotrophic and methylotrophic bacterium that utilizes dichloromethane","volume":"51","author":"Doronina","year":"2001","journal-title":"Int J Syst Evol Microbiol"},{"key":"r8","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1134\/S0026261711040047","article-title":"Phytosymbiosis of aerobic methylobacteria: new facts and views","volume":"80","author":"Fedorov","year":"2011","journal-title":"Microbiol"},{"key":"r9","first-page":"164","article-title":"phylip \u2013 phylogeny inference package (version 3.2)","volume":"5","author":"Felsenstein","year":"1989","journal-title":"Cladistics"},{"key":"r10","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp Ser"},{"key":"r11","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.syapm.2007.03.001","article-title":"Hansschlegelia plantiphila gen. nov. sp. nov., a new aerobic restricted facultative methylotrophic bacterium associated with plants","volume":"30","author":"Ivanova","year":"2007","journal-title":"Syst Appl Microbiol"},{"key":"r10a","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1099\/ijs.0.038075-0","article-title":"Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species","volume":"62","author":"Kim","year":"2012","journal-title":"Int J Syst Evol Microbiol"},{"key":"r12","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1271\/bbb.59.648","article-title":"Purification and characterization of the new \u03b3-glutamylmethylamide dissimilating enzyme system from Methylophaga sp. AA-30","volume":"59","author":"Kimura","year":"1995","journal-title":"Biosci Biotechnol Biochem"},{"key":"r13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2009.01681.x","article-title":"Aerobic methanol-oxidizing bacteria in soil","volume":"300","author":"Kolb","year":"2009","journal-title":"FEMS Microbiol Lett"},{"key":"r14","first-page":"115","article-title":"16S\/23S rRNA sequencing","volume-title":"Nucleic Acid Techniques in Bacterial Systematics","author":"Lane","year":"1991"},{"key":"r15","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1099\/ijs.0.020925-0","article-title":"Methylopila jiangsuensis sp. nov., an aerobic, facultatively methylotrophic bacterium","volume":"61","author":"Li","year":"2011","journal-title":"Int J Syst Evol Microbiol"},{"key":"r16","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J Biol Chem"},{"key":"r17","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/S0022-2836(61)80047-8","article-title":"A procedure for the isolation of deoxyribonucleic acid from microorganisms","volume":"3","author":"Marmur","year":"1961","journal-title":"J Mol Biol"},{"key":"r18","doi-asserted-by":"crossref","first-page":"3218","DOI":"10.1128\/AEM.63.8.3218-3224.1997","article-title":"The methanol dehydrogenase structural gene mxaF and its use as a functional gene probe for methanotrophs and methylotrophs","volume":"63","author":"McDonald","year":"1997","journal-title":"Appl Environ Microbiol"},{"key":"r19","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1042\/bj0550416","article-title":"The colorimetric estimation of formaldehyde by means of the Hantzsch reaction","volume":"55","author":"Nash","year":"1953","journal-title":"Biochem J"},{"key":"r20","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1038\/ismej.2007.65","article-title":"Stable-isotope probing implicates Methylophaga spp and novel Gammaproteobacteria in marine methanol and methylamine metabolism","volume":"1","author":"Neufeld","year":"2007","journal-title":"ISME J"},{"key":"r21","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1111\/j.1365-2672.1976.tb00640.x","article-title":"The thermal denaturation of partly purified bacterial deoxyribonucleic acid and its taxonomic applications","volume":"41","author":"Owen","year":"1976","journal-title":"J Appl Bacteriol"},{"key":"r22","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook","year":"2001"},{"key":"r23","volume-title":"Identification of bacteria by gas chromatography of fatty acids. MIDI Technical Note 101","author":"Sasser","year":"1990"},{"key":"r24","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res"},{"key":"r25","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1111\/j.1574-6968.1986.tb01290.x","article-title":"Metabolism of non-motile obligately methylotrophic bacteria","volume":"33","author":"Trotsenko","year":"1986","journal-title":"FEMS Microbiol Lett"},{"key":"r26","first-page":"569","article-title":"treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment","volume":"10","author":"Van de Peer","year":"1994","journal-title":"Comput Appl Biosci"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijs.0.042028-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,29]],"date-time":"2022-01-29T05:59:58Z","timestamp":1643435998000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijs.0.042028-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,1]]},"references-count":26,"journal-issue":{"issue":"Pt_5","published-print":{"date-parts":[[2013,5,1]]}},"URL":"https:\/\/doi.org\/10.1099\/ijs.0.042028-0","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,1]]}}}