{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:23:26Z","timestamp":1759335806372,"version":"3.37.3"},"reference-count":34,"publisher":"Microbiology Society","issue":"Pt_7","funder":[{"name":"Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Disease for the Hong Kong Special Administrative Region Department of Health"},{"name":"Providence Foundation Limited"},{"name":"Shaw Foundation"},{"name":"University Development Fund, The University of Hong Kong"},{"name":"Committee for Research and Conference Grant, The University of Hong Kong"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,7,1]]},"abstract":"A bacterium, HKU30T<\/jats:sup>, was isolated from the infected tissue of a patient with wound infection after puncture by a fish fin. Cells are facultative anaerobic, non-spore-forming, non-motile, Gram-positive cocci arranged in chains. Colonies were non-haemolytic. The strain was catalase, oxidase, urease and Voges\u2013Proskauer test negative. It reacted with Lancefield\u2019s group G antisera and was resistant to optochin. It grew on bile aesculin agar and in 5\u200a% NaCl. It was unidentified by three commercial identification systems. 16S rRNA gene sequence analysis indicated that the bacterium shared 98.2, 97.7, 97.4 and 97.1\u200a% nucleotide identities withStreptococcus iniae<\/jats:italic><\/jats:ext-link><\/jats:named-content>,Streptococcus pseudoporcinus<\/jats:italic><\/jats:ext-link><\/jats:named-content>,Streptococcus parauberis<\/jats:italic><\/jats:ext-link><\/jats:named-content>andStreptococcus uberis<\/jats:italic><\/jats:ext-link><\/jats:named-content>, respectively. The DNA G+C content was 35.6\u00b10.9 mol% (mean\u00b1sd<\/jats:sc>). In view of the occupational exposure of the patient, an epidemiological study was performed to isolate the bacterium from marine fish. Two strains, with similar phenotypic and genotypic characteristics to those of HKU30T<\/jats:sup>, were isolated from a three-lined tongue sole (Cynoglossus abbreviatus<\/jats:italic>) and an olive flounder (Paralichthys olivaceus<\/jats:italic>) respectively. Phylogenetic analysis of four additional housekeeping genes,groEL<\/jats:italic>,gyrB<\/jats:italic>,sodA<\/jats:italic>andrpoB<\/jats:italic>, showed that the three isolates formed a distinct branch among known species of the genusStreptococcus<\/jats:italic><\/jats:ext-link><\/jats:named-content>, being most closely related toS. parauberis<\/jats:italic><\/jats:ext-link><\/jats:named-content>(CCUG 39954T<\/jats:sup>). DNA\u2013DNA hybridization demonstrated\u2264<\/jats:italic>53.8\u200a% DNA relatedness between the three isolates and related species of the genusStreptococcus<\/jats:italic><\/jats:ext-link><\/jats:named-content>. A novel species,Streptococcus hongkongensis<\/jats:italic>sp. nov., is proposed. The type strain is HKU30T<\/jats:sup>(\u200a=\u200aDSM 26014T<\/jats:sup>\u200a=\u200aCECT 8154T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.045120-0","type":"journal-article","created":{"date-parts":[[2012,12,22]],"date-time":"2012-12-22T07:04:03Z","timestamp":1356159843000},"page":"2570-2576","source":"Crossref","is-referenced-by-count":26,"title":["Streptococcus hongkongensis sp. nov., isolated from a patient with an infected puncture wound and from a marine flatfish"],"prefix":"10.1099","volume":"63","author":[{"given":"Susanna K. P.","family":"Lau","sequence":"first","affiliation":[{"name":"State Key Laboratory of Emerging Infectious Diseases, Hong Kong, PR China"},{"name":"Department of Microbiology, The University of Hong Kong, Hong Kong, PR China"},{"name":"Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, PR China"},{"name":"Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shirly O. T.","family":"Curreem","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Hong Kong, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cherry C. N.","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pathology, Kwong Wah Hospital, Hong Kong, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ami M. 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