{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:05:26Z","timestamp":1740135926040,"version":"3.37.3"},"reference-count":43,"publisher":"Microbiology Society","issue":"Pt_7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,7,1]]},"abstract":"<jats:p>A micro-organism resembling members of the genus<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus\" xlink:type=\"simple\"><jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.5991\" xlink:type=\"simple\"><jats:italic>Dermatophilus<\/jats:italic><\/jats:ext-link><\/jats:named-content>, strain W254<jats:sup>T<\/jats:sup>, which was isolated from the submandibular lymph node of a pig, and an additional 16 strains isolated from swine tonsils, were studied to establish their taxonomic status. Although all 17 strains were isolated anaerobically under an atmosphere of 100\u200a% CO<jats:sub>2<\/jats:sub>, all of them were aerotolerant anaerobes. The micro-organisms showed at least five cellular morphologies: (i) a radially protrusive thallus, which proliferated into tuber-like cells; (ii) segmentation in both tubers and thallus followed by multilocule formation, (iii) development of coccoid forms in the locules; (iv) a change from the coccoid forms to zoospores; (v) resting cells, which were able to develop into protrusive thalli again. The micro-organisms were positive for nitrate reduction, but negative for catalase, indole production, hydrolysis of urea and gelatin liquefaction. Milk was not decomposed and none of the strains was haemolytic. A total of 16 compounds, including glucose, were utilized as sole carbon sources and seven compounds, including<jats:sc>l<\/jats:sc>-arabinose, were not utilized. Three out of the 17 strains were subjected to further studies. The micro-organisms had<jats:italic>meso<\/jats:italic>-diaminopimelic acid in their peptidoglycan and galactose, glucose, madurose and a trace of mannose in their whole-cell sugar patterns. The major phospholipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol.Cellular fatty acids were C<jats:sub>15\u200a:\u200a0<\/jats:sub>(35.7\u201323.1\u200a%), C<jats:sub>16\u200a:\u200a0<\/jats:sub>(5.9\u20132.4\u200a%) C<jats:sub>17\u200a:\u200a0<\/jats:sub>(62.9\u201339.5\u200a%), C<jats:sub>17\u200a:\u200a1<\/jats:sub>(24.4\u20130\u200a%) and C<jats:sub>18\u200a:\u200a0<\/jats:sub>(3\u20131.6\u200a%). The predominant menaquinone was MK-8 (H<jats:sub>4<\/jats:sub>). The G+C content of the DNA was 69.6\u201371.8 mol%. Analysis of the 16S rRNA gene sequences showed that the strains clustered with the type strains of members of the family<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"family\" xlink:type=\"simple\"><jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.5990\" xlink:type=\"simple\"><jats:italic>Dermatophilaceae<\/jats:italic><\/jats:ext-link><\/jats:named-content>. Based on the polyphasic taxonomic characterization carried out, all 17 strains are considered to belong to a novel species in a new genus, for which the name<jats:italic>Tonsilliphilus suis<\/jats:italic>gen. nov., sp. nov. is proposed. The type strain of the type species is W254<jats:sup>T<\/jats:sup>(\u200a=\u200aATCC 35846<jats:sup>T<\/jats:sup>\u200a=\u200aCCM 3774<jats:sup>T<\/jats:sup>\u200a=\u200aDSM 21880<jats:sup>T<\/jats:sup>\u200a=\u200aJCM 15727<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.045237-0","type":"journal-article","created":{"date-parts":[[2012,12,16]],"date-time":"2012-12-16T02:14:27Z","timestamp":1355624067000},"page":"2545-2552","source":"Crossref","is-referenced-by-count":10,"title":["Tonsilliphilus suis gen. nov., sp. nov., causing tonsil infections in pigs"],"prefix":"10.1099","volume":"63","author":[{"given":"Ryozo","family":"Azuma","sequence":"first","affiliation":[{"name":"2-7-33 Higashi-tokura, Kokubunji-city, Tokyo 185-0002, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bak","family":"Ung-Bok","sequence":"additional","affiliation":[{"name":"1002, 808-dong Suri-Apt, Sanbon-dong, Gunpo-city, Kyong-gi-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Murakami","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Health, Department of Animal Science, Tokyo University of Agriculture, 1737 Funako Atugi-city, 243-0034, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Ishiwata","sequence":"additional","affiliation":[{"name":"Technical Research Institute, Nishimatu Construction Co. Ltd Nakatu, Aikawa-machi Kanagawa-Pref., 243-0303, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Osaki","sequence":"additional","affiliation":[{"name":"Safety Research team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba-city 305-0856, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuaki","family":"Shimada","sequence":"additional","affiliation":[{"name":"Safety Research team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba-city 305-0856, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuichiro","family":"Ito","sequence":"additional","affiliation":[{"name":"1-3 Bangai, Tamiya, Ushiku-city, 300-1236, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eichi","family":"Miyagawa","sequence":"additional","affiliation":[{"name":"Agricultural Microbiology, Faculty of Dairy Science, Rakuno Gakuen University, 582 Bunkyodai, Midorimachi, Ebetu, 069-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Makino","sequence":"additional","affiliation":[{"name":"Yakult Central Institute for Microbiological Research 1796 Yaho, Kunitachi-city, 186-8650, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuji","family":"Kudo","sequence":"additional","affiliation":[{"name":"Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoko","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Kitasato Life Science Institute, Kitasato University. 5-9-1 Shirogane, Minatoku, Tokyo 108-8641, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuya","family":"Yano","sequence":"additional","affiliation":[{"name":"Japan BCG Laboratory, 3-1-5 Matuyama, Kiyose, Tokyo 204-0303, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryo","family":"Murata","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Health, Department of Animal Science, Tokyo University of Agriculture, 1737 Funako Atugi-city, 243-0034, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiji","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Chiba Prefectural Institute of Public Health, 666-2 Nitona, Chuo, Chiba, 260-8715 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/S0007-1935(17)34540-2","article-title":"Dermatophilus congolensis. 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