{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:50:05Z","timestamp":1787010605359,"version":"build-2736575974"},"reference-count":33,"publisher":"Microbiology Society","issue":"Pt_7","funder":[{"name":"Ministry of Science and Technology","award":["2006AA10211"],"award-info":[{"award-number":["2006AA10211"]}]},{"name":"Ministry of Agriculture","award":["201003066"],"award-info":[{"award-number":["201003066"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31171504"],"award-info":[{"award-number":["31171504"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Foundation","doi-asserted-by":"crossref","award":["317000-X91102"],"award-info":[{"award-number":["317000-X91102"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,7,1]]},"abstract":"\n Five nitrogen-fixing bacterial strains (SP1\n T<\/jats:sup>\n , NN143, NN144, NN208 and HX148) were isolated from stem, root or rhizosphere soil of sugar cane (\n Saccharum officinarum<\/jats:italic>\n L.) plants. Cells were Gram-negative, motile, rods with peritrichous flagella. DNA G+C content was 55.0\u00b10.5 mol%. Sequence determinations and phylogenetic analysis of 16S rRNA gene and\n rpoB<\/jats:italic>\n indicated that the strains were affiliated with the genus\n \n \n Enterobacter<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n and most closely related to\n \n \n E. radicincitans<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n DSM 16656\n T<\/jats:sup>\n and\n \n \n E. oryzae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n LMG 24251\n T<\/jats:sup>\n . Fluorimetric determination of thermal denaturation temperatures after DNA\u2013DNA hybridization, enterobacterial repetitive intergenic consensus PCR and matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry differentiated the whole-genome, genotype and protein profiles from those of\n \n \n E. radicincitans<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n and\n \n \n E. oryzae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n . The strains\u2019 cell fatty acid composition differentiated them from\n \n \n E. radicincitans<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n and\n \n \n E. oryzae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n by containing a higher level of summed feature 2 (C\n 16\u200a:\u200a1<\/jats:sub>\n \u03c97\n c<\/jats:italic>\n and\/or C\n 16\u200a:\u200a1<\/jats:sub>\n \u03c96\n c<\/jats:italic>\n ) and a lower level of C\n 17\u200a:\u200a0<\/jats:sub>\n cyclo. Their physiological and biochemical profiles differentiated them from\n \n \n E. radicincitans<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n by being positive for methyl red test, ornithine decarboxylase and utilization of putrescine,\n d<\/jats:sc>\n -arabitol,\n l<\/jats:sc>\n -fucose and methyl \u03b1-\n d<\/jats:sc>\n -glucoside and being negative for arginine dihydrolase, and differentiated them from\n \n \n E. oryzae<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n by being positive for aesculin hydrolysis and utilization of putrescine,\n d<\/jats:sc>\n -arabitol and\n l<\/jats:sc>\n -rhamnose and being negative for arginine dihydrolase, lysine decarboxylase and utilization of mucate. The five strains therefore represent a novel species, for which the name\n Enterobacter sacchari<\/jats:italic>\n sp. nov. is proposed, with the type strain SP1\n T<\/jats:sup>\n (\u200a=\u200aCGMCC 1.12102\n T<\/jats:sup>\n \u200a=\u200aLMG 26783\n T<\/jats:sup>\n ).\n <\/jats:p>","DOI":"10.1099\/ijs.0.045500-0","type":"journal-article","created":{"date-parts":[[2013,1,5]],"date-time":"2013-01-05T01:40:32Z","timestamp":1357350032000},"page":"2577-2582","source":"Crossref","is-referenced-by-count":50,"title":["Enterobacter sacchari sp. nov., a nitrogen-fixing bacterium associated with sugar cane (Saccharum officinarum L.)"],"prefix":"10.1099","volume":"63","author":[{"given":"Bo","family":"Zhu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Daxuedong Road 100, Nanning 530005, China"},{"name":"Guangxi Key Laboratory of Sugarcane Genetic Improvement, Guangxi Academy of Agricultural Sciences, Daxuedong Road 172, Nanning 530007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunjin","family":"Hu","sequence":"additional","affiliation":[{"name":"Microbiology Institute, Guangxi Academy of Agricultural Sciences, Daxuedong Road 174, Nanning 530007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Litao","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Daxuedong Road 100, Nanning 530005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianli","family":"An","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanlin","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangrui","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Daxuedong Road 100, Nanning 530005, China"},{"name":"Guangxi Key Laboratory of Sugarcane Genetic Improvement, Guangxi Academy of Agricultural Sciences, Daxuedong Road 172, Nanning 530007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped blast and psi-blast: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res"},{"key":"r2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/BF02370096","article-title":"A new acid-tolerant nitrogen-fixing bacterium associated with 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