{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:40:30Z","timestamp":1790199630501,"version":"4.1.0"},"reference-count":35,"publisher":"Microbiology Society","issue":"Pt_8","funder":[{"DOI":"10.13039\/501100005416","name":"Norwegian Research Council","doi-asserted-by":"crossref","award":["210923"],"award-info":[{"award-number":["210923"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"crossref"}]},{"name":"German Ministry of Education and Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,8,1]]},"abstract":"A methanogenic archaeon, strain SMA-21T<\/jats:sup>, was isolated from a permafrost-affected soil by serial dilution in liquid medium. The cells were non-motile, stained Gram-negative and grew as irregular cocci with a diameter of 1.3\u20132.5 \u00b5m. Optimal growth was observed at 28 \u00b0C, pH 7.8 and 0.02 M NaCl. The strain grew on H2<\/jats:sub>\/CO2<\/jats:sub>, methanol and acetate, but not on formate, ethanol, 2-butanol, 2-propanol, monomethylamine, dimethylamine, trimethylamine or dimethyl sulfide. Major membrane lipids of strain SMA-21T<\/jats:sup> were archaeol phosphatidylglycerol, archaeol phosphatidylethanolamine and the corresponding hydroxyarchaeol compounds. The G+C content of the genomic DNA was 40.9 mol%. The 16S rRNA gene sequence was closely related to those of \n \n Methanosarcina mazei<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> DSM 2053T<\/jats:sup> (similarity 99.9\u200a%) and \n \n Methanosarcina horonobensis<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> HB-1T<\/jats:sup> (similarity 98.7\u200a%). On basis of the level of DNA\u2013DNA hybridization (22.1\u200a%) between strain SMA-21T<\/jats:sup> and \n \n Methanosarcina mazei<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> DSM 2053T<\/jats:sup> as well as of phenotypic and genotypic differences, strain SMA-21T<\/jats:sup> was assigned to a novel species of the genus \n \n Methanosarcina<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>, for which the name \n \n Methanosarcina<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> \n soligelidi<\/jats:italic> sp. nov. is proposed. The type strain is SMA-21T<\/jats:sup> (\u200a=\u200aDSM 20065T<\/jats:sup>\u200a=\u200aJCM 18468).<\/jats:p>","DOI":"10.1099\/ijs.0.046565-0","type":"journal-article","created":{"date-parts":[[2013,2,2]],"date-time":"2013-02-02T03:34:31Z","timestamp":1359776071000},"page":"2986-2991","source":"Crossref","is-referenced-by-count":74,"title":["Methanosarcina\n \n \n soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic archaeon from a Siberian permafrost-affected soil"],"prefix":"10.1099","volume":"63","author":[{"given":"Dirk","family":"Wagner","sequence":"first","affiliation":[{"name":"Helmholtz Centre Potsdam, German Research Centre for Geosciences (GFZ), Section 4.5 Geomicrobiology, Telegrafenberg, 14473 Potsdam, Germany"},{"name":"Alfred Wegener Institute for Polar and Marine Research, Research Unit Potsdam, Telegrafenberg A45, 14473 Potsdam, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janosch","family":"Schirmack","sequence":"additional","affiliation":[{"name":"Alfred Wegener Institute for Polar and Marine Research, Research Unit Potsdam, Telegrafenberg A45, 14473 Potsdam, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Ganzert","sequence":"additional","affiliation":[{"name":"University of Troms\u00f8, Department for Arctic and Marine Biology, Dramsveien 201, 9037 Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daria","family":"Morozova","sequence":"additional","affiliation":[{"name":"Alfred Wegener Institute for Polar and Marine Research, Research Unit Potsdam, Telegrafenberg A45, 14473 Potsdam, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Mangelsdorf","sequence":"additional","affiliation":[{"name":"Helmholtz Centre Potsdam, German Research Centre for Geosciences (GFZ), 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