{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:06:36Z","timestamp":1790791596940,"version":"4.1.0"},"reference-count":21,"publisher":"Microbiology Society","issue":"Pt_11","funder":[{"name":"Medical faculty of Ume\u00e5 University"},{"name":"County of V\u00e4sterbotten"},{"name":"Fund for Biotechnology-oriented Basic Science at Ume\u00e5 University"},{"name":"Swedish Research Council, Natural Sciences and Engineering Sciences","award":["2010-5669"],"award-info":[{"award-number":["2010-5669"]}]},{"name":"Swedish Research Council, Natural Sciences and Engineering Sciences","award":["222720-2"],"award-info":[{"award-number":["222720-2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,11,1]]},"abstract":"Five obligately anaerobic, Gram-stain-negative, saccharolytic and proteolytic, non-spore-forming bacilli (strains CD3\u200a:\u200a27, CD3\u200a:\u200a28T<\/jats:sup>, CD3\u200a:\u200a33, CD3\u200a:\u200a32 and CD3\u200a:\u200a34) are described. All five strains were isolated from the small intestine of a female child with coeliac disease. Cells of the five strains were short rods or coccoid cells with longer filamentous forms seen sporadically. The organisms produced acetic acid and succinic acid as major metabolic end products. Phylogenetic analysis based on comparative 16S rRNA gene sequence analysis revealed close relationships between CD3\u200a:\u200a27, CD3\u200a:\u200a28T<\/jats:sup> and CD3\u200a:\u200a33, between CD3\u200a:\u200a32 and \n \n Prevotella histicola<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> CCUG 55407T<\/jats:sup>, and between CD3\u200a:\u200a34 and \n \n Prevotella melaninogenica<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> CCUG 4944BT<\/jats:sup>. Strains CD3\u200a:\u200a27, CD3\u200a:\u200a28T<\/jats:sup> and CD3\u200a:\u200a33 were clearly different from all recognized species within the genus \n \n Prevotella<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content> and related most closely to but distinct from \n \n P. melaninogenica<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>. Based on 16S rRNA, RNA polymerase \u03b2-subunit (rpoB<\/jats:italic>) and 60 kDa chaperonin protein subunit (cpn60<\/jats:italic>) gene sequencing, and phenotypic, chemical and biochemical properties, strains CD3\u200a:\u200a27, CD3\u200a:\u200a28T<\/jats:sup> and CD3\u200a:\u200a33 are considered to represent a novel species within the genus \n \n Prevotella<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>, for which the name Prevotella jejuni<\/jats:italic> sp. nov. is proposed. Strain CD3\u200a:\u200a28T<\/jats:sup> (\u200a=\u200aCCUG 60371T<\/jats:sup>\u200a=\u200aDSM 26989T<\/jats:sup>) is the type strain of the proposed novel species. All five strains were able to form homologous aggregates, in which tube-like structures were connecting individual bacteria cells. The five strains were able to bind to human intestinal carcinoma cell lines at 37 \u00b0C.<\/jats:p>","DOI":"10.1099\/ijs.0.052647-0","type":"journal-article","created":{"date-parts":[[2013,6,22]],"date-time":"2013-06-22T03:55:03Z","timestamp":1371873303000},"page":"4218-4223","source":"Crossref","is-referenced-by-count":39,"title":["Prevotella jejuni sp. nov., isolated from the small intestine of a child with coeliac disease"],"prefix":"10.1099","volume":"63","author":[{"given":"Maria E.","family":"Hedberg","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Immunology, Ume\u00e5 University, SE-90187 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Israelsson","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Immunology, Ume\u00e5 University, SE-90187 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward R. B.","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Sahlgrenska Academy of the University of Gothenburg, SE-40530 G\u00f6teborg, Sweden"},{"name":"CCUG \u2013 Culture Collection University of Gothenburg, Department of Clinical Bacteriology, Sahlgrenska University Hospital, SE-41345 G\u00f6teborg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liselott","family":"Svensson-Stadler","sequence":"additional","affiliation":[{"name":"CCUG \u2013 Culture Collection University of Gothenburg, Department of Clinical Bacteriology, Sahlgrenska University Hospital, SE-41345 G\u00f6teborg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Nyunt","family":"Wai","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Ume\u00e5 University, SE-90187 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grzegorz","family":"Pietz","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Immunology, Ume\u00e5 University, SE-90187 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olof","family":"Sandstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Pediatrics, Ume\u00e5 University, SE-90187 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olle","family":"Hernell","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Pediatrics, Ume\u00e5 University, SE-90187 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Louise","family":"Hammarstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Immunology, Ume\u00e5 University, SE-90187 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sten","family":"Hammarstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Immunology, Ume\u00e5 University, SE-90187 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.2217\/fmb.10.126","article-title":"New insights into Prevotella diversity and medical microbiology","volume":"5","author":"Alauzet","year":"2010","journal-title":"Future Microbiol"},{"key":"r2","doi-asserted-by":"crossref","first-page":"5002","DOI":"10.1128\/JB.00542-10","article-title":"The human oral microbiome","volume":"192","author":"Dewhirst","year":"2010","journal-title":"J Bacteriol"},{"key":"r3","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1099\/ijs.0.65656-0","article-title":"Prevotella histicola sp. nov., isolated from the human oral cavity","volume":"58","author":"Downes","year":"2008","journal-title":"Int J Syst Evol Microbiol"},{"key":"r4","article-title":"List of prokaryotic names with standing in nomenclature","author":"Euz\u00e9by","year":"2013"},{"key":"r5","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1111\/j.1572-0241.2004.04157.x","article-title":"Presence of bacteria and innate immunity of intestinal epithelium in childhood celiac disease","volume":"99","author":"Forsberg","year":"2004","journal-title":"Am J Gastroenterol"},{"key":"r6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.mimet.2006.11.017","article-title":"A dual fluorescence flow cytometric analysis of bacterial adherence to mammalian host cells","volume":"69","author":"Hara-Kaonga","year":"2007","journal-title":"J Microbiol Methods"},{"key":"r7","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.anaerobe.2005.02.004","article-title":"Properties of hemagglutination by Prevotella melaninogenica","volume":"11","author":"Haraldsson","year":"2005","journal-title":"Anaerobe"},{"key":"r8","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1099\/ijs.0.64778-0","article-title":"Prevotella copri sp. nov. and Prevotella stercorea sp. nov., isolated from human faeces","volume":"57","author":"Hayashi","year":"2007","journal-title":"Int J Syst Evol Microbiol"},{"key":"r9","doi-asserted-by":"crossref","first-page":"2685","DOI":"10.1099\/ijs.0.033613-0","article-title":"Lachnoanaerobaculum gen. nov., a new genus in the Lachnospiraceae; characterization of Lachnoanaerobaculum umeaense gen. nov., sp. nov., isolated from the human small intestine, Lachnoanaerobaculum orale sp. nov., isolated from saliva and reclassification of Eubacterium saburreum (Pr\u00e9vot) Holdeman and Moore 1970 as Lachnoanaerobaculum saburreum comb. nov.","volume":"62","author":"Hedberg","year":"2012","journal-title":"Int J Syst Evol Microbiol"},{"key":"r10","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1111\/j.1651-2227.2000.tb01210.x","article-title":"Epidemic of coeliac disease in Swedish children","volume":"89","author":"Ivarsson","year":"2000","journal-title":"Acta Paediatr"},{"key":"r11","volume-title":"Wadsworth-KTL anaerobic bacteriology manual","author":"Jousimies-Somer","year":"2002"},{"key":"r12","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1128\/JCM.27.1.182-184.1989","article-title":"Detection of sialidase (neuraminidase) by using 2\u2032-(4-methylumbelliferyl)\u03b1-d-N-acetylneuraminic acid in a filter paper spot test","volume":"27","author":"Moncla","year":"1989","journal-title":"J Clin Microbiol"},{"key":"r13","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1186\/1471-2431-12-194","article-title":"Early infections are associated with increased risk for celiac disease: an incident case-referent study","volume":"12","author":"Myl\u00e9us","year":"2012","journal-title":"BMC Pediatr"},{"key":"r14","doi-asserted-by":"crossref","first-page":"S76","DOI":"10.1017\/S0007114512005521","article-title":"Host genotype, intestinal microbiota and inflammatory disorders","volume":"109","author":"Olivares","year":"2013","journal-title":"Br J Nutr"},{"key":"r15","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1542\/peds.2007-2989","article-title":"Difference in celiac disease risk between Swedish birth cohorts suggests an opportunity for primary prevention","volume":"122","author":"Olsson","year":"2008","journal-title":"Pediatrics"},{"key":"r16","doi-asserted-by":"crossref","first-page":"3058","DOI":"10.1038\/ajg.2009.524","article-title":"Proximal small intestinal microbiota and identification of rod-shaped bacteria associated with childhood celiac disease","volume":"104","author":"Ou","year":"2009","journal-title":"Am J Gastroenterol"},{"key":"r17","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1099\/jmm.0.020420-0","article-title":"Usefulness of the hsp60 gene for the identification and classification of Gram-negative anaerobic rods","volume":"59","author":"Sakamoto","year":"2010","journal-title":"J Med Microbiol"},{"key":"r18","doi-asserted-by":"crossref","first-page":"D5","DOI":"10.1093\/nar\/gkp967","article-title":"Database resources of the National Center for Biotechnology Information","volume":"38","author":"Sayers","year":"2010","journal-title":"Nucleic Acids Res"},{"key":"r19","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1099\/00207713-40-2-205","article-title":"Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides","volume":"40","author":"Shah","year":"1990","journal-title":"Int J Syst Bacteriol"},{"key":"r20","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1099\/00207713-44-4-846","article-title":"Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology","volume":"44","author":"Stackebrandt","year":"1994","journal-title":"Int J Syst Bacteriol"},{"key":"r21","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.syapm.2008.07.002","article-title":"Reclassification of Rhodobium marinum and Rhodobium pfennigii as Afifella marina gen. nov. comb. nov. and Afifella pfennigii comb. nov., a new genus of photoheterotrophic Alphaproteobacteria and emended descriptions of Rhodobium, Rhodobium orientis and Rhodobium gokarnense","volume":"31","author":"Urdiain","year":"2008","journal-title":"Syst Appl Microbiol"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijs.0.052647-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T15:42:03Z","timestamp":1598629323000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijs.0.052647-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,1]]},"references-count":21,"journal-issue":{"issue":"Pt_11","published-print":{"date-parts":[[2013,11,1]]}},"URL":"https:\/\/doi.org\/10.1099\/ijs.0.052647-0","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,1]]}}}