{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:51:17Z","timestamp":1791150677446,"version":"4.1.0"},"reference-count":34,"publisher":"Microbiology Society","issue":"Pt_2","funder":[{"name":"Emil Racovi\u0163\u0103 Institute of Speleology, Bucharest"},{"name":"\u2018Group for Underwater and Speleological Exploration (GESS)\u2019 Bucharest"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,2,1]]},"abstract":"<jats:p>A novel strain of methanogenic archaea, designated MC-20<jats:sup>T<\/jats:sup>, was isolated from the anoxic sediment of a subsurface lake in Movile Cave, Mangalia, Romania. Cells were non-motile, Gram-stain-negative rods 3.5\u20134.0 \u00b5m in length and 0.6\u20130.7 \u00b5m in width, and occurred either singly or in short chains. Strain MC-20<jats:sup>T<\/jats:sup>was able to utilize H<jats:sub>2<\/jats:sub>\/CO<jats:sub>2<\/jats:sub>, formate, 2-propanol and 2-butanol as substrate, but not acetate, methanol, ethanol, dimethyl sulfide, monomethylamine, dimethylamine or trimethylamine. Neither trypticase peptone nor yeast extract was required for growth. The major membrane lipids of strain MC-20<jats:sup>T<\/jats:sup>were archaeol phosphatidylethanolamine and diglycosyl archaeol, while archaeol phosphatidylinositol and glycosyl archaeol were present only in minor amounts. Optimal growth was observed at 33 \u00b0C, pH 7.4 and 0.08 M NaCl. Based on phylogenetic analysis of 16S rRNA gene sequences, strain MC-20<jats:sup>T<\/jats:sup>was closely affiliated with<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"species\" xlink:type=\"simple\"><jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.104\" xlink:type=\"simple\"><jats:italic>Methanobacterium oryzae<\/jats:italic><\/jats:ext-link><\/jats:named-content>FPi<jats:sup>T<\/jats:sup>(similarity 97.1\u200a%) and<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"species\" xlink:type=\"simple\"><jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.23143\" xlink:type=\"simple\"><jats:italic>Methanobacterium lacus<\/jats:italic><\/jats:ext-link><\/jats:named-content>17A1<jats:sup>T<\/jats:sup>(97.0\u200a%). The G+C content of the genomic DNA was 33.0 mol%. Based on phenotypic and genotypic differences, strain MC-20<jats:sup>T<\/jats:sup>was assigned to a novel species of the genus<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus\" xlink:type=\"simple\"><jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/dx.doi.org\/10.1601\/nm.94\" xlink:type=\"simple\"><jats:italic>Methanobacterium<\/jats:italic><\/jats:ext-link><\/jats:named-content>for which the name<jats:italic>Methanobacterium movilense<\/jats:italic>sp. nov. is proposed. The type strain is MC-20<jats:sup>T<\/jats:sup>(\u200a=\u200aDSM 26032<jats:sup>T<\/jats:sup>\u200a=\u200aJCM 18470<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijs.0.057224-0","type":"journal-article","created":{"date-parts":[[2013,10,10]],"date-time":"2013-10-10T16:03:59Z","timestamp":1381421039000},"page":"522-527","source":"Crossref","is-referenced-by-count":54,"title":["Methanobacterium movilense sp. nov., a hydrogenotrophic, secondary-alcohol-utilizing methanogen from the anoxic sediment of a subsurface lake"],"prefix":"10.1099","volume":"64","author":[{"given":"Janosch","family":"Schirmack","sequence":"first","affiliation":[{"name":"Alfred Wegener Institute for Polar and Marine Research, Research Unit Potsdam, Telegrafenberg A45, 14473 Potsdam, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Mangelsdorf","sequence":"additional","affiliation":[{"name":"GFZ German Research Center for Geosciences, Section 4.3 Organic Geochemistry, Telegrafenberg, Potsdam, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Ganzert","sequence":"additional","affiliation":[{"name":"Finnish Forest Research Institute, Rovaniemi Unit, Etel\u00e4ranta 55, 96100 Rovaniemi, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Sand","sequence":"additional","affiliation":[{"name":"University of Duisburg-Essen, Biofilm Centre, Aquatic Biotechnology, Geibelstrasse 41, 47057 Duisburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Hillebrand-Voiculescu","sequence":"additional","affiliation":[{"name":"Emil Racovi\u0163\u0103 Institute of Speleology, Str. 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