{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:33:58Z","timestamp":1792794838845,"version":"4.5.0"},"reference-count":42,"publisher":"Microbiology Society","issue":"Pt_12","funder":[{"DOI":"10.13039\/501100000277","name":"UK Department of Environment, Food and Rural Affairs","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000277","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006021","name":"Direction G\u00e9n\u00e9rale de l\u2019Armement","doi-asserted-by":"crossref","award":["Brucella REI2010 34 0003"],"award-info":[{"award-number":["Brucella REI2010 34 0003"]}],"id":[{"id":"10.13039\/501100006021","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008751","name":"Federal Institute for Risk Assessment","doi-asserted-by":"crossref","award":["BfR project no. 1322-503"],"award-info":[{"award-number":["BfR project no. 1322-503"]}],"id":[{"id":"10.13039\/501100008751","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Office of Research Infrastructure Programs (ORIP) of the National Institutes of Health","award":["P51\u00a0OD011133"],"award-info":[{"award-number":["P51\u00a0OD011133"]}],"id":[{"id":"https:\/\/ror.org\/01jdyfj45","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Office of Research Infrastructure Programs (ORIP) of the National Institutes of Health","award":["1\u00a0C06\u00a0RR014578"],"award-info":[{"award-number":["1\u00a0C06\u00a0RR014578"]}],"id":[{"id":"https:\/\/ror.org\/01jdyfj45","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Office of Research Infrastructure Programs (ORIP) of the National Institutes of Health","award":["1\u00a0C06\u00a0RR015456"],"award-info":[{"award-number":["1\u00a0C06\u00a0RR015456"]}],"id":[{"id":"https:\/\/ror.org\/01jdyfj45","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Center for Research Resources, National Institutes of Health"},{"name":"South-west National Primate Research Center","award":["P51\u00a0RR013986"],"award-info":[{"award-number":["P51\u00a0RR013986"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"\n Two Gram-negative, non-motile, non-spore-forming coccoid bacteria (strains F8\/08-60\n T<\/jats:sup>\n and F8\/08-61) isolated from clinical specimens obtained from baboons (\n Papio<\/jats:italic>\n spp.) that had delivered stillborn offspring were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence similarities, both strains, which possessed identical sequences, were assigned to the genus\n \n \n Brucella<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n . This placement was confirmed by extended multilocus sequence analysis (MLSA), where both strains possessed identical sequences, and whole-genome sequencing of a representative isolate. All of the above analyses suggested that the two strains represent a novel lineage within the genus\n \n \n Brucella<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n . The strains also possessed a unique profile when subjected to the phenotyping approach classically used to separate species of the genus\n \n \n Brucella<\/jats:italic>\n ,\n <\/jats:ext-link>\n <\/jats:named-content>\n reacting only with\n Brucella<\/jats:italic>\n A monospecific antiserum, being sensitive to the dyes thionin and fuchsin, being lysed by bacteriophage Wb, Bk\n 2<\/jats:sub>\n and Fi phage at routine test dilution (RTD) but only partially sensitive to bacteriophage Tb, and with no requirement for CO\n 2<\/jats:sub>\n and no production of H\n 2<\/jats:sub>\n S but strong urease activity. Biochemical profiling revealed a pattern of enzyme activity and metabolic capabilities distinct from existing species of the genus\n \n \n Brucella<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n . Molecular analysis of the\n omp2<\/jats:italic>\n locus genes showed that both strains had a novel combination of two highly similar\n omp2b<\/jats:italic>\n gene copies. The two strains shared a unique fingerprint profile of the multiple-copy\n Brucella<\/jats:italic>\n -specific element IS\n 711.<\/jats:italic>\n Like MLSA, a multilocus variable number of tandem repeat analysis (MLVA) showed that the isolates clustered together very closely, but represent a distinct group within the genus\n \n \n Brucella<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n . Isolates F8\/08-60\n T<\/jats:sup>\n and F8\/08-61 could be distinguished clearly from all known species of the genus\n \n \n Brucella<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n and their biovars by both phenotypic and molecular properties. Therefore, by applying the species concept for the genus\n \n \n Brucella<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n suggested by the ICSP Subcommittee on the Taxonomy of\n Brucella<\/jats:italic>\n , they represent a novel species within the genus\n \n \n Brucella<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n , for which the name\n Brucella papionis<\/jats:italic>\n sp. nov. is proposed, with the type strain F8\/08-60\n T<\/jats:sup>\n (\u200a=\u200aNCTC 13660\n T<\/jats:sup>\n \u200a=\u200aCIRMBP 0958\n T<\/jats:sup>\n ).\n <\/jats:p>","DOI":"10.1099\/ijs.0.065482-0","type":"journal-article","created":{"date-parts":[[2014,9,21]],"date-time":"2014-09-21T20:08:52Z","timestamp":1411330132000},"page":"4120-4128","source":"Crossref","is-referenced-by-count":178,"title":["Brucella papionis sp. nov., isolated from baboons (Papio spp.)"],"prefix":"10.1099","volume":"64","author":[{"given":"Adrian M.","family":"Whatmore","sequence":"first","affiliation":[{"name":"OIE\/WHO\/FAO Brucellosis Reference Laboratory, Department of Bacteriology, Animal Health and Veterinary Laboratories Agency (AHVLA), Woodham Lane, Addlestone KT15\u00a03NB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Davison","sequence":"additional","affiliation":[{"name":"Animal Health and Veterinary Laboratories Agency (AHVLA), Polwhele, Truro TR4\u00a09AD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Cloeckaert","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Fran\u00e7ois Rabelais de Tours, UMR1282 Infectiologie et Sant\u00e9 Publique, F-37000 Tours, France"},{"name":"INRA, UMR1282 Infectiologie et Sant\u00e9 Publique, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sascha","family":"Al Dahouk","sequence":"additional","affiliation":[{"name":"Federal Institute for Risk Assessment (BfR), Diedersdorfer Weg 1, D-12277 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel S.","family":"Zygmunt","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Fran\u00e7ois Rabelais de Tours, UMR1282 Infectiologie et Sant\u00e9 Publique, F-37000 Tours, France"},{"name":"INRA, UMR1282 Infectiologie et Sant\u00e9 Publique, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon D.","family":"Brew","sequence":"additional","affiliation":[{"name":"OIE\/WHO\/FAO Brucellosis Reference Laboratory, Department of Bacteriology, Animal Health and Veterinary Laboratories Agency (AHVLA), Woodham Lane, Addlestone KT15\u00a03NB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorraine L.","family":"Perrett","sequence":"additional","affiliation":[{"name":"OIE\/WHO\/FAO Brucellosis Reference Laboratory, Department of Bacteriology, Animal Health and Veterinary Laboratories Agency (AHVLA), Woodham Lane, Addlestone KT15\u00a03NB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark S.","family":"Koylass","sequence":"additional","affiliation":[{"name":"OIE\/WHO\/FAO Brucellosis Reference Laboratory, Department of Bacteriology, Animal Health and Veterinary Laboratories Agency (AHVLA), Woodham Lane, Addlestone KT15\u00a03NB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Vergnaud","sequence":"additional","affiliation":[{"name":"DGA\/MRIS \u2013 Mission pour la Recherche et l\u2019Innovation Scientifique, F-92221 Bagneux, France"},{"name":"CNRS, F-91405 Orsay, France"},{"name":"Universit\u00e9 Paris-Sud, Institut de G\u00e9n\u00e9tique et Microbiologie, UMR 8621, F-91405 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Quance","sequence":"additional","affiliation":[{"name":"Mycobacteria and Brucella Section, National Veterinary Services Laboratories, USDA-APHIS, Ames, 1920 Dayton Ave, Ames, IA 50010, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger C.","family":"Scholz","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D-80937 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward J.","family":"Dick","sequence":"additional","affiliation":[{"name":"Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gene","family":"Hubbard","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia E.","family":"Schlabritz-Loutsevitch","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.mimet.2006.12.015","article-title":"Evaluation of Brucella MLVA typing for human brucellosis","volume":"69","author":"Al Dahouk","year":"2007","journal-title":"J Microbiol Methods"},{"key":"r2","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1186\/1471-2180-10-269","article-title":"Differential phenotyping of Brucella species using a newly developed semi-automated metabolic system","volume":"10","author":"Al Dahouk","year":"2010","journal-title":"BMC Microbiol"},{"key":"r3","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.1128\/AEM.06351-11","article-title":"Intraspecies biodiversity of the genetically homologous species Brucella microti","volume":"78","author":"Al Dahouk","year":"2012","journal-title":"Appl Environ Microbiol"},{"key":"r4","volume-title":"Techniques for the Brucellosis Laboratory","author":"Alton","year":"1988"},{"key":"r5","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1186\/1471-2148-11-200","article-title":"The genome sequence of Brucella pinnipedialis B2\/94 sheds light on the evolutionary history of the genus Brucella","volume":"11","author":"Audic","year":"2011","journal-title":"BMC Evol Biol"},{"key":"r6","first-page":"271","article-title":"Detection of Brucella melitensis and Brucella abortus by DNA amplification","volume":"95","author":"Baily","year":"1992","journal-title":"J Trop Med Hyg"},{"key":"r7","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1128\/JCM.32.11.2660-2666.1994","article-title":"Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR","volume":"32","author":"Bricker","year":"1994","journal-title":"J Clin Microbiol"},{"key":"r8","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1128\/JCM.38.3.1258-1262.2000","article-title":"Molecular characterization of Brucella strains isolated from marine mammals","volume":"38","author":"Bricker","year":"2000","journal-title":"J Clin Microbiol"},{"key":"r9","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1177\/104063870301500413","article-title":"Evaluation of the Brucella abortus species-specific polymerase chain reaction assay, an improved version of the Brucella AMOS polymerase chain reaction assay for cattle","volume":"15","author":"Bricker","year":"2003","journal-title":"J Vet Diagn Invest"},{"key":"r10","volume-title":"Brucellosis Diagnosis, Standard Laboratory Techniques","author":"Brinley Morgan","year":"1978"},{"key":"r11","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1099\/00207713-25-1-83","article-title":"Proposal for minimal standards for descriptions of new species and biotypes of the genus Brucella","volume":"25","author":"Corbel","year":"1975","journal-title":"Int J Syst Bacteriol"},{"key":"r12","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1186\/1471-2180-8-224","article-title":"Phenotypic and molecular characterisation of Brucella isolates from marine mammals","volume":"8","author":"Dawson","year":"2008","journal-title":"BMC Microbiol"},{"key":"r13","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1128\/JCM.01494-07","article-title":"Novel Brucella strain (BO1) associated with a prosthetic breast implant infection","volume":"46","author":"De","year":"2008","journal-title":"J Clin Microbiol"},{"key":"r14","doi-asserted-by":"crossref","first-page":"3753","DOI":"10.1128\/AEM.07509-11","article-title":"Isolation of potentially novel Brucella spp. from frogs","volume":"78","author":"Eisenberg","year":"2012","journal-title":"Appl Environ Microbiol"},{"key":"r15","doi-asserted-by":"crossref","first-page":"3085","DOI":"10.1128\/JCM.38.8.3085-3086.2000","article-title":"Validation of the abbreviated Brucella AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the vaccine strains, 19 and RB51","volume":"38","author":"Ewalt","year":"2000","journal-title":"J Clin Microbiol"},{"key":"r16","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1638\/2011-0005R2.1","article-title":"Abscesses associated with a Brucella inopinata-like bacterium in a big-eyed tree frog (Leptopelis vermiculatus)","volume":"43","author":"Fischer","year":"2012","journal-title":"J Zoo Wildl Med"},{"key":"r17","doi-asserted-by":"crossref","first-page":"2688","DOI":"10.1099\/ijs.0.65269-0","article-title":"Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts","volume":"57","author":"Foster","year":"2007","journal-title":"Int J Syst Evol Microbiol"},{"key":"r18","doi-asserted-by":"crossref","first-page":"2858","DOI":"10.1016\/j.vaccine.2006.09.063","article-title":"Assessment of genetic stability of Brucella melitensis Rev 1 vaccine strain by multiple-locus variable-number tandem repeat analysis","volume":"25","author":"Garc\u00eda-Yoldi","year":"2007","journal-title":"Vaccine"},{"key":"r19","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1128\/AEM.03755-13","article-title":"Examination of taxonomic uncertainties surrounding Brucella abortus bv. 7 by phenotypic and molecular approaches","volume":"80","author":"Garin-Bastuji","year":"2014","journal-title":"Appl Environ Microbiol"},{"key":"r20","doi-asserted-by":"crossref","first-page":"3649","DOI":"10.1128\/JCM.42.8.3649-3654.2004","article-title":"Use of 16S rRNA gene sequencing for rapid confirmatory identification of Brucella isolates","volume":"42","author":"Gee","year":"2004","journal-title":"J Clin Microbiol"},{"key":"r21","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.prevetmed.2011.04.007","article-title":"Brucellosis at the animal\/ecosystem\/human interface at the beginning of the 21st century","volume":"102","author":"Godfroid","year":"2011","journal-title":"Prev Vet Med"},{"key":"r22","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/0378-1119(93)90236-V","article-title":"Sequence and characterization of an insertion sequence, IS711, from Brucella ovis","volume":"133","author":"Halling","year":"1993","journal-title":"Gene"},{"key":"r23","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1093\/infdis\/52.3.374","article-title":"Dissociation in the genus Brucella","volume":"52","author":"Henry","year":"1933","journal-title":"J Infect Dis"},{"key":"r24","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.vetmic.2012.12.036","article-title":"A novel taxon within the genus Actinobacillus isolated from alpaca (Vicugna pacos) in the United Kingdom","volume":"163","author":"Hunt","year":"2013","journal-title":"Vet Microbiol"},{"key":"r25","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1471-2180-6-9","article-title":"Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay","volume":"6","author":"Le Fl\u00e8che","year":"2006","journal-title":"BMC Microbiol"},{"key":"r26","doi-asserted-by":"crossref","first-page":"3484","DOI":"10.1128\/JCM.00837-08","article-title":"Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains","volume":"46","author":"L\u00f3pez-Go\u00f1i","year":"2008","journal-title":"J Clin Microbiol"},{"key":"r27","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1186\/1471-2180-9-145","article-title":"MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis","volume":"9","author":"Maquart","year":"2009","journal-title":"BMC Microbiol"},{"key":"r28","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1099\/ijs.0.64349-0","article-title":"International Committee on Systematics of Prokaryotes; Subcommittee on the Taxonomy of Brucella: minutes of the meeting, 17 September 2003, Pamplona, Spain","volume":"56","author":"Osterman","year":"2006","journal-title":"Int J Syst Evol Microbiol"},{"key":"r29","doi-asserted-by":"crossref","first-page":"3265","DOI":"10.1099\/00221287-139-12-3265","article-title":"Identification and sequence analysis of IS6501, an insertion sequence in Brucella spp.: relationship between genomic structure and the number of IS6501 copies","volume":"139","author":"Ouahrani","year":"1993","journal-title":"J Gen Microbiol"},{"key":"r30","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1111\/j.1600-0684.2008.00314.x","article-title":"A novel Brucella isolate in association with two cases of stillbirth in non-human primates \u2013 first report","volume":"38","author":"Schlabritz-Loutsevitch","year":"2009","journal-title":"J Med Primatol"},{"key":"r31","doi-asserted-by":"crossref","first-page":"149","DOI":"10.20506\/rst.32.1.2189","article-title":"Molecular characterisation of Brucella species","volume":"32","author":"Scholz","year":"2013","journal-title":"Rev Sci Tech"},{"key":"r32","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1099\/ijs.0.65356-0","article-title":"Brucella microti sp. nov., isolated from the common vole Microtus arvalis","volume":"58","author":"Scholz","year":"2008","journal-title":"Int J Syst Evol Microbiol"},{"key":"r33","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1099\/ijs.0.011148-0","article-title":"Brucella inopinata sp. nov., isolated from a breast implant infection","volume":"60","author":"Scholz","year":"2010","journal-title":"Int J Syst Evol Microbiol"},{"key":"r34","doi-asserted-by":"crossref","first-page":"5837","DOI":"10.1128\/AEM.00620-10","article-title":"Characterization of novel Brucella strains originating from wild native rodent species in North Queensland, Australia","volume":"76","author":"Tiller","year":"2010a","journal-title":"Appl Environ Microbiol"},{"key":"r35","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1471-2180-10-23","article-title":"Identification of an unusual Brucella strain (BO2) from a lung biopsy in a 52 year-old patient with chronic destructive pneumonia","volume":"10","author":"Tiller","year":"2010b","journal-title":"BMC Microbiol"},{"key":"r36","doi-asserted-by":"crossref","first-page":"e5519","DOI":"10.1371\/journal.pone.0005519","article-title":"Genome degradation in Brucella ovis corresponds with narrowing of its host range and tissue tropism","volume":"4","author":"Tsolis","year":"2009","journal-title":"PLoS ONE"},{"key":"r37","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1099\/00207713-35-3-292","article-title":"Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization","volume":"35","author":"Verger","year":"1985","journal-title":"Int J Syst Bacteriol"},{"key":"r38","doi-asserted-by":"crossref","first-page":"e00246-12","DOI":"10.1128\/mBio.00246-12","article-title":"Comparative genomics of early-diverging Brucella strains reveals a novel lipopolysaccharide biosynthesis pathway","volume":"3","author":"Wattam","year":"2012","journal-title":"MBio"},{"key":"r39","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1128\/JB.01091-13","article-title":"Comparative phylogenomics and evolution of the Brucellae reveal a path to virulence","volume":"196","author":"Wattam","year":"2014","journal-title":"J Bacteriol"},{"key":"r40","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1016\/j.meegid.2009.07.001","article-title":"Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens","volume":"9","author":"Whatmore","year":"2009","journal-title":"Infect Genet Evol"},{"key":"r41","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/1471-2180-7-34","article-title":"Characterisation of the genetic diversity of Brucella by multilocus sequencing","volume":"7","author":"Whatmore","year":"2007","journal-title":"BMC Microbiol"},{"key":"r42","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1099\/00207713-48-1-179","article-title":"Reclassification of Shewanella putrefaciens Owen\u2019s genomic group II as Shewanella baltica sp. nov.","volume":"48","author":"Ziemke","year":"1998","journal-title":"Int J Syst Bacteriol"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijs.0.065482-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T07:16:48Z","timestamp":1598599008000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijs.0.065482-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,1]]},"references-count":42,"journal-issue":{"issue":"Pt_12","published-print":{"date-parts":[[2014,12,1]]}},"URL":"https:\/\/doi.org\/10.1099\/ijs.0.065482-0","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,1]]}}}