{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:09:29Z","timestamp":1792786169577,"version":"4.5.0"},"reference-count":45,"publisher":"Microbiology Society","issue":"Pt_12","funder":[{"name":"French Ministry of Health"},{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services","award":["HHSN272200900007C"],"award-info":[{"award-number":["HHSN272200900007C"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"\n A group of strains representing species of the genus\n \n \n Leptospira<\/jats:italic>\n ,\n <\/jats:ext-link>\n <\/jats:named-content>\n isolated from patients with leptospirosis in Mayotte (Indian Ocean), were previously found to be considerably divergent from other known species of the genus\n \n \n Leptospira<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n . This was inferred from sequence analysis of\n rrs<\/jats:italic>\n (16S rRNA) and other genetic loci and suggests that they belong to a novel species. Two strains from each serogroup currently identified within this novel species were studied. Spirochaete, aerobic, motile, helix-shaped strains grew well at 30\u201337 \u00b0C, but not at 13 \u00b0C or in the presence of 8-azaguanine. Draft genomes of the strains were also analysed to study the DNA relatedness with other species of the genus\n \n \n Leptospira<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n . The new isolates formed a distinct clade, which was most closely related to\n \n \n Leptospira borgpetersenii<\/jats:italic>\n <\/jats:ext-link>\n <\/jats:named-content>\n , in multilocus sequence analysis using concatenated sequences of the genes\n rpoB<\/jats:italic>\n ,\n recA<\/jats:italic>\n ,\n fusA<\/jats:italic>\n ,\n gyrB<\/jats:italic>\n ,\n leuS<\/jats:italic>\n and\n sucA<\/jats:italic>\n . Analysis of average nucleotide identity and genome-to-genome distances, which have recently been proposed as reliable substitutes for classical DNA\u2013DNA hybridization, further confirmed that these isolates should be classified as representatives of a novel species. The G+C content of the genomic DNA was 39.5 mol%. These isolates are considered to represent a novel species, for which the name\n Leptospira mayottensis<\/jats:italic>\n sp. nov. is proposed, with 200901116\n T<\/jats:sup>\n (\u200a=\u200aCIP 110703\n T<\/jats:sup>\n \u200a=\u200aDSM 28999\n T<\/jats:sup>\n ) as the type strain.\n <\/jats:p>","DOI":"10.1099\/ijs.0.066597-0","type":"journal-article","created":{"date-parts":[[2014,9,24]],"date-time":"2014-09-24T01:55:12Z","timestamp":1411523712000},"page":"4061-4067","source":"Crossref","is-referenced-by-count":117,"title":["Leptospira mayottensis sp. nov., a pathogenic species of the genus Leptospira isolated from humans"],"prefix":"10.1099","volume":"64","author":[{"given":"Pascale","family":"Bourhy","sequence":"first","affiliation":[{"name":"Institut Pasteur, Biology of Spirochetes Unit, National Reference Centre and WHO Collaborating Center for Leptospirosis, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Collet","sequence":"additional","affiliation":[{"name":"Hospital Centre of Mayotte (CHM), Mayotte, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Brisse","sequence":"additional","affiliation":[{"name":"CNRS, UMR 3525, Paris, France"},{"name":"Institut Pasteur, Microbial Evolutionary Genomics Unit, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Picardeau","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Biology of Spirochetes Unit, National Reference Centre and WHO Collaborating Center for Leptospirosis, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"r1","doi-asserted-by":"crossref","first-page":"142","DOI":"10.4056\/sigs.541628","article-title":"Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs","volume":"2","author":"Auch","year":"2010a","journal-title":"Stand Genomic Sci"},{"key":"r2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.4056\/sigs.531120","article-title":"Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison","volume":"2","author":"Auch","year":"2010b","journal-title":"Stand Genomic Sci"},{"key":"r3","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1128\/JCM.05931-11","article-title":"Human leptospira isolates circulating in Mayotte (Indian Ocean) have unique serological and molecular features","volume":"50","author":"Bourhy","year":"2012","journal-title":"J Clin Microbiol"},{"key":"r4","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1099\/00207713-49-2-839","article-title":"Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies","volume":"49","author":"Brenner","year":"1999","journal-title":"Int J Syst Bacteriol"},{"key":"r5","doi-asserted-by":"crossref","first-page":"14560","DOI":"10.1073\/pnas.0603979103","article-title":"Genome reduction in Leptospira borgpetersenii reflects limited transmission potential","volume":"103","author":"Bulach","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"r6","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.gene.2012.09.074","article-title":"Sequence of Leptospira santarosai serovar Shermani genome and prediction of virulence-associated genes","volume":"511","author":"Chou","year":"2012","journal-title":"Gene"},{"key":"r7","doi-asserted-by":"crossref","first-page":"134","DOI":"10.4269\/ajtmh.2012.12-0102","article-title":"Similarities in Leptospira serogroup and species distribution in animals and humans in the Indian ocean island of Mayotte","volume":"87","author":"Desvars","year":"2012","journal-title":"Am J Trop Med Hyg"},{"key":"r8","doi-asserted-by":"crossref","first-page":"2783","DOI":"10.1111\/mec.12777","article-title":"Diversification of an emerging pathogen in a biodiversity hotspot: Leptospira in endemic small mammals of Madagascar","volume":"23","author":"Dietrich","year":"2014","journal-title":"Mol Ecol"},{"key":"r9","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"muscle: multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res"},{"key":"r10","first-page":"45","article-title":"Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80","volume":"26","author":"Ellinghausen","year":"1965","journal-title":"Am J Vet Res"},{"key":"r11","doi-asserted-by":"crossref","first-page":"3892","DOI":"10.1128\/IAI.00466-12","article-title":"Leptospira interrogans catalase is required for resistance to H2O2 and for virulence","volume":"80","author":"Eshghi","year":"2012","journal-title":"Infect Immun"},{"key":"r12","volume-title":"Leptospira and leptospirosis","author":"Faine","year":"1999"},{"key":"r13","doi-asserted-by":"crossref","first-page":"628","DOI":"10.4269\/ajtmh.2008.78.628","article-title":"Evaluation of a modified pulsed-field gel electrophoresis approach for the identification of Leptospira serovars","volume":"78","author":"Galloway","year":"2008","journal-title":"Am J Trop Med Hyg"},{"key":"r14","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1002\/cm.970090202","article-title":"Motility of the spirochete Leptospira.","volume":"9","author":"Goldstein","year":"1988","journal-title":"Cell Motil Cytoskeleton"},{"key":"r15","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1099\/ijs.0.64483-0","article-title":"DNA-DNA hybridization values and their relationship to whole-genome sequence similarities","volume":"57","author":"Goris","year":"2007","journal-title":"Int J Syst Evol Microbiol"},{"key":"r16","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1080\/10635150390235520","article-title":"A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood","volume":"52","author":"Guindon","year":"2003","journal-title":"Syst Biol"},{"key":"r17","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1111\/j.1365-2958.2010.07262.x","article-title":"Leptospira: a spirochaete with a hybrid outer membrane","volume":"77","author":"Haake","year":"2010","journal-title":"Mol Microbiol"},{"key":"r18","doi-asserted-by":"crossref","first-page":"1696","DOI":"10.1128\/JCM.30.7.1696-1702.1992","article-title":"Pulsed-field gel electrophoresis of NotI digests of leptospiral DNA: a new rapid method of serovar identification","volume":"30","author":"Herrmann","year":"1992","journal-title":"J Clin Microbiol"},{"key":"r19","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1099\/00207713-29-3-245","article-title":"Leptospiraceae, a new Family to include Leptospira Noguchi 1917 and Leptonema, gen. nov.","volume":"29","author":"Hovind-Hougen","year":"1979","journal-title":"Int J Syst Bacteriol"},{"key":"r20","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1128\/JB.94.1.27-31.1967","article-title":"Differentiation of pathogenic and saprophytic leptospires I. Growth at low temperatures","volume":"94","author":"Johnson","year":"1967","journal-title":"J Bacteriol"},{"key":"r21","doi-asserted-by":"crossref","first-page":"1618","DOI":"10.1128\/JB.88.6.1618-1623.1964","article-title":"Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine","volume":"88","author":"Johnson","year":"1964","journal-title":"J Bacteriol"},{"key":"r22","volume-title":"Classification of the Species Leptospira interrogans and History of its Serovars","author":"Kmety","year":"1993"},{"key":"r23","doi-asserted-by":"crossref","first-page":"2567","DOI":"10.1073\/pnas.0409727102","article-title":"Genomic insights that advance the species definition for prokaryotes","volume":"102","author":"Konstantinidis","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"r24","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1099\/ijs.0.63783-0","article-title":"Leptospira broomii sp. nov., isolated from humans with leptospirosis","volume":"56","author":"Levett","year":"2006","journal-title":"Int J Syst Evol Microbiol"},{"key":"r25","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0076-6879(67)12133-2","article-title":"Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA","volume":"12B","author":"Mandel","year":"1968","journal-title":"Methods Enzymol"},{"key":"r26","doi-asserted-by":"crossref","first-page":"e213","DOI":"10.1371\/journal.pntd.0000213","article-title":"Human leptospirosis caused by a new, antigenically unique Leptospira associated with a Rattus species reservoir in the Peruvian Amazon","volume":"2","author":"Matthias","year":"2008","journal-title":"PLoS Negl Trop Dis"},{"key":"r27","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/1471-2105-14-60","article-title":"Genome sequence-based species delimitation with confidence intervals and improved distance functions","volume":"14","author":"Meier-Kolthoff","year":"2012","journal-title":"BMC Bioinformatics"},{"key":"r28","doi-asserted-by":"crossref","first-page":"3510","DOI":"10.1128\/JCM.00670-06","article-title":"Species-specific identification of Leptospiraceae by 16S rRNA gene sequencing","volume":"44","author":"Morey","year":"2006","journal-title":"J Clin Microbiol"},{"key":"r29","doi-asserted-by":"crossref","first-page":"2164","DOI":"10.1128\/JB.186.7.2164-2172.2004","article-title":"Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis","volume":"186","author":"Nascimento","year":"2004","journal-title":"J Bacteriol"},{"key":"r30","doi-asserted-by":"crossref","first-page":"6101","DOI":"10.1128\/JB.173.19.6101-6109.1991","article-title":"Phylogenetic analysis of the spirochetes","volume":"173","author":"Paster","year":"1991","journal-title":"J Bacteriol"},{"key":"r31","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1099\/00207713-48-3-851","article-title":"Leptospira fainei sp. nov., isolated from pigs in Australia","volume":"48","author":"Perolat","year":"1998","journal-title":"Int J Syst Bacteriol"},{"key":"r32","doi-asserted-by":"crossref","first-page":"e1607","DOI":"10.1371\/journal.pone.0001607","article-title":"Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis","volume":"3","author":"Picardeau","year":"2008","journal-title":"PLoS ONE"},{"key":"r33","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1099\/00207713-42-2-215","article-title":"Genetic characterization of pathogenic Leptospira species by DNA hybridization","volume":"42","author":"Ramadass","year":"1992","journal-title":"Int J Syst Bacteriol"},{"key":"r34","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1038\/nature01597","article-title":"Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing","volume":"422","author":"Ren","year":"2003","journal-title":"Nature"},{"key":"r35","doi-asserted-by":"crossref","first-page":"e1853","DOI":"10.1371\/journal.pntd.0001853","article-title":"Whole genome analysis of Leptospira licerasiae provides insight into leptospiral evolution and pathogenicity","volume":"6","author":"Ricaldi","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"r36","doi-asserted-by":"crossref","first-page":"19126","DOI":"10.1073\/pnas.0906412106","article-title":"Shifting the genomic gold standard for the prokaryotic species definition","volume":"106","author":"Richter","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"key":"r37","doi-asserted-by":"crossref","first-page":"2457","DOI":"10.1099\/ijs.0.047233-0","article-title":"Leptospira idonii sp. nov., isolated from environmental water","volume":"63","author":"Saito","year":"2013","journal-title":"Int J Syst Evol Microbiol"},{"key":"r38","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1128\/JCM.24.3.484-486.1986","article-title":"Newly recognized Leptospira species (\u201cLeptospira inadai\u201d serovar lyme) isolated from human skin","volume":"24","author":"Schmid","year":"1986","journal-title":"J Clin Microbiol"},{"key":"r39","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1099\/ijs.0.64947-0","article-title":"Leptospira wolffii sp. nov., isolated from a human with suspected leptospirosis in Thailand","volume":"58","author":"Slack","year":"2008","journal-title":"Int J Syst Evol Microbiol"},{"key":"r40","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1099\/ijs.0.002766-0","article-title":"Leptospira kmetyi sp. nov., isolated from an environmental source in Malaysia","volume":"59","author":"Slack","year":"2009","journal-title":"Int J Syst Evol Microbiol"},{"key":"r41","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1099\/ijs.0.047324-0","article-title":"Classification of Leptospira genomospecies 1, genomospecies 3, genomospecies 4 and genomospecies 5 as Leptospira alstonii sp. nov., Leptospira vanthielii sp. nov., Leptospira terpstrae sp. nov., Leptospira yanagawae sp. nov., respectively","volume":"63","author":"Smythe","year":"2012","journal-title":"Int J Syst Evol Microbiol"},{"key":"r42","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1099\/ijs.0.016949-0","article-title":"Notes on the characterization of prokaryote strains for taxonomic purposes","volume":"60","author":"Tindall","year":"2010","journal-title":"Int J Syst Evol Microbiol"},{"key":"r43","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1099\/00207713-37-4-463","article-title":"International Committee on Ststematic Bacteriology. Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics","volume":"37","author":"Wayne","year":"1987","journal-title":"Int J Syst Bacteriol"},{"key":"r44","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1471-2148-1-8","article-title":"Genome trees constructed using five different approaches suggest new major bacterial clades","volume":"1","author":"Wolf","year":"2001","journal-title":"BMC Evol Biol"},{"key":"r45","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1099\/00207713-37-4-407","article-title":"Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species","volume":"37","author":"Yasuda","year":"1987","journal-title":"Int J Syst Bacteriol"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijs.0.066597-0?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,2]],"date-time":"2024-06-02T17:58:10Z","timestamp":1717351090000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijs.0.066597-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,1]]},"references-count":45,"journal-issue":{"issue":"Pt_12","published-print":{"date-parts":[[2014,12,1]]}},"URL":"https:\/\/doi.org\/10.1099\/ijs.0.066597-0","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,1]]}}}