{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:04:14Z","timestamp":1791180254219,"version":"4.1.0"},"reference-count":36,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,11,1]]},"abstract":"<jats:p>A mycoplasma was isolated from the sputum of an immunodeficient patient with recurrent bronchitis. The isolate designated strain A39<jats:sup>T<\/jats:sup> was very fastidious and atypical for a mycoplasma in its colonial appearance. Classical biochemical tests for mycoplasma speciation could not differentiate the isolate from the pathogens <jats:italic>Mycoplasma pneumoniae<\/jats:italic> and <jats:italic>Mycoplasma genitalium<\/jats:italic> and serological identification as a recognized <jats:italic>Mycoplasma<\/jats:italic> species was lacking. Specific PCR detection for these two species was negative. Subsequently, other strains were isolated from human patients that appeared to be similar to strain A39<jats:sup>T<\/jats:sup> in their physiological and genetic characteristics. Analysis of the 16S rRNA gene placed strain A39<jats:sup>T<\/jats:sup> and other isolates in the pneumoniae group of mycoplasmas, with the highest sequence similarity to <jats:italic>Mycoplasma testudinis<\/jats:italic> (96\u00b78\u200a%), but with only 93\u00b70\u200a% similarity to <jats:italic>M. pneumoniae<\/jats:italic> and <jats:italic>M. genitalium<\/jats:italic>. Examination of the 16S\u201323S rRNA internally transcribed spacer sequence, protein electrophoresis profile, genome size and serological reactions indicated that this organism represents a novel species, for which the name <jats:italic>Mycoplasma amphoriforme<\/jats:italic> sp. nov. is proposed, with strain A39<jats:sup>T<\/jats:sup> (=NCTC 11740<jats:sup>T<\/jats:sup>=ATCC BAA-992<jats:sup>T<\/jats:sup>) as the type strain.<\/jats:p>","DOI":"10.1099\/ijs.0.63269-0","type":"journal-article","created":{"date-parts":[[2005,11,9]],"date-time":"2005-11-09T21:07:56Z","timestamp":1131570476000},"page":"2589-2594","source":"Crossref","is-referenced-by-count":34,"title":["Mycoplasma amphoriforme sp. nov., isolated from a patient with chronic bronchopneumonia"],"prefix":"10.1099","volume":"55","author":[{"given":"D. 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