{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:23:37Z","timestamp":1790555017589,"version":"4.1.0"},"reference-count":19,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,3,1]]},"abstract":"<jats:p>The prevalence of leptospirosis is generally high in domestic animals and rodents in Tanzania. Identification of <jats:italic>Leptospira<\/jats:italic> isolates from cattle was carried out to establish prevalent <jats:italic>Leptospira<\/jats:italic> serovars. Serological typing was done based on monoclonal antibodies and the standard cross-agglutination absorption test. Molecular typing involved pathogenic- and saprophytic-specific PCRs and a PCR specifically amplifying DNA from the species <jats:italic>Leptospira kirschneri<\/jats:italic>. DNA fingerprinting with primers derived from sequences of insertion elements IS<jats:italic>1500<\/jats:italic> and IS<jats:italic>1533<\/jats:italic> was carried out. Both serological and molecular characterization indicated that one of the <jats:italic>Leptospira<\/jats:italic> isolates, coded RM1, represents a new serovar of the species <jats:italic>L. kirschneri<\/jats:italic> of serogroup Icterohaemorrhagiae. The serovar name Sokoine is proposed for this new <jats:italic>Leptospira<\/jats:italic> isolate.<\/jats:p>","DOI":"10.1099\/ijs.0.63278-0","type":"journal-article","created":{"date-parts":[[2006,3,2]],"date-time":"2006-03-02T20:23:34Z","timestamp":1141331014000},"page":"593-597","source":"Crossref","is-referenced-by-count":36,"title":["New Leptospira serovar Sokoine of serogroup Icterohaemorrhagiae from cattle in Tanzania"],"prefix":"10.1099","volume":"56","author":[{"given":"G. F.","family":"Mgode","sequence":"first","affiliation":[{"name":"Sokoine University of Agriculture, Pest Management Centre, PO Box 3110, Morogoro, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. S.","family":"Machang'u","sequence":"additional","affiliation":[{"name":"Sokoine University of Agriculture, Pest Management Centre, PO Box 3110, Morogoro, Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. G.","family":"Goris","sequence":"additional","affiliation":[{"name":"Royal Tropical Institute, Department of Biomedical Research, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Engelbert","sequence":"additional","affiliation":[{"name":"Royal Tropical Institute, Department of Biomedical Research, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sondij","sequence":"additional","affiliation":[{"name":"Royal Tropical Institute, Department of Biomedical Research, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A.","family":"Hartskeerl","sequence":"additional","affiliation":[{"name":"Royal Tropical Institute, Department of Biomedical Research, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1128\/JCM.28.3.495-503.1990","article-title":"Rapid and simple method for purification of nucleic acids","volume":"28","author":"Boom","year":"1990","journal-title":"J Clin Microbiol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1099\/00207713-49-2-839","article-title":"Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies","volume":"49","author":"Brenner","year":"1999","journal-title":"Int J Syst Bacteriol"},{"key":"R3","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1128\/AEM.25.6.976-980.1973","article-title":"Improved microtechnique for the leptospiral agglutination test","volume":"25","author":"Cole","year":"1973","journal-title":"Appl Microbiol"},{"key":"R4","first-page":"260","article-title":"Serological typing methods of leptospires","volume":"11","author":"Dikken","year":"1978","journal-title":"Methods Microbiol"},{"key":"R5","first-page":"67","volume-title":"Guidelines for Leptospirosis Control","author":"Faine","year":"1982"},{"key":"R6","volume-title":"Leptospira and Leptospirosis","author":"Faine","year":"1999"},{"key":"R7","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1099\/00221287-139-8-1691","article-title":"Detection of seven species of pathogenic leptospires by PCR using two sets of primers","volume":"139","author":"Gravekamp","year":"1993","journal-title":"J Gen Microbiol"},{"key":"R8","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1128\/JB.94.1.27-31.1967","article-title":"Differentiation of pathogenic and saprophytic leptospires. 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