{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T14:05:38Z","timestamp":1791036338144,"version":"4.1.0"},"reference-count":28,"publisher":"Microbiology Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,5,1]]},"abstract":"<jats:p>Eight mycobacterial strains isolated during an 11\u2005year period from the sputum of independent patients with various pulmonary disorders and, in one case, from a lymph node of a young girl, were found to present identical features. Phenotypic and genotypic characteristics revealed that the most closely related species to these test isolates were<jats:italic>Mycobacterium triplex<\/jats:italic>and<jats:italic>Mycobacterium lentiflavum<\/jats:italic>. However, the lipids of the cell wall of the test isolates differed from those of the latter species by TLC and presented unique profiles by both GC and HPLC. Genotypic analysis showed that they had unique 16S rRNA gene and internal transcribed spacer (ITS) sequences, and could be differentiated from all other mycobacterial strains by PCR restriction analysis of<jats:italic>hsp65<\/jats:italic>. The strains presented high resistance to antimycobacterial drugs. The name<jats:italic>Mycobacterium florentinum<\/jats:italic>sp. nov. is proposed for this taxon, with strain FI-93171<jats:sup>T<\/jats:sup>(=DSM 44852<jats:sup>T<\/jats:sup>=CIP 108409<jats:sup>T<\/jats:sup>) as the type strain.<\/jats:p>","DOI":"10.1099\/ijs.0.63485-0","type":"journal-article","created":{"date-parts":[[2005,5,5]],"date-time":"2005-05-05T19:13:47Z","timestamp":1115320427000},"page":"1101-1106","source":"Crossref","is-referenced-by-count":40,"title":["Mycobacterium florentinum sp. nov., isolated from humans"],"prefix":"10.1099","volume":"55","author":[{"given":"Enrico","family":"Tortoli","sequence":"first","affiliation":[{"name":"Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Rindi","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, Medical Biotechnologies, Infectivology and Epidemiology, University of Pisa, 56127 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khye S.","family":"Goh","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de la Tuberculose et des Mycobact\u00e9ries, Institut Pasteur de Guadeloupe, 97165 Pointe-\u00e0-Pitre, Guadeloupe, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marja L.","family":"Katila","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Kuopio University Hospital, 70211 Kuopio, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Mariottini","sequence":"additional","affiliation":[{"name":"Genetics and Cytogenetics Unit, Careggi Hospital, 50134 Florence, Italy"},{"name":"Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romano","family":"Mattei","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, Campo di Marte Hospital, 55100 Lucca, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianna","family":"Mazzarelli","sequence":"additional","affiliation":[{"name":"Microbiological and Virological Serum-immunology Laboratory, Careggi Hospital, 50134 Florence, Italy"},{"name":"Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sini","family":"Suomalainen","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pirjo","family":"Torkko","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Microbiology, National Public Health Institute, 70211 Kuopio, Finland"},{"name":"Department of Clinical Microbiology, Kuopio University Hospital, 70211 Kuopio, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nalin","family":"Rastogi","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de la Tuberculose et des Mycobact\u00e9ries, Institut Pasteur de Guadeloupe, 97165 Pointe-\u00e0-Pitre, Guadeloupe, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1099\/00207713-43-4-841","article-title":"Mycobacterium genavense sp. nov","volume":"43","author":"B\u00f6ttger","year":"1993","journal-title":"Int J Syst Bacteriol"},{"key":"R2","volume-title":"Standardized Method for HPLC Identification of Mycobacteria","author":"Butler","year":"1996"},{"key":"R3","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1099\/ijs.0.02760-0","article-title":"Mycobacterium parmense sp. nov","volume":"54","author":"Fanti","year":"2004","journal-title":"Int J Syst Evol Microbiol"},{"key":"R4","volume-title":"phylip (phylogeny inference package), version 3.5c. 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