{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:00:19Z","timestamp":1791597619122,"version":"4.3.4"},"reference-count":16,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,4,1]]},"abstract":"<jats:p>A taxonomic study was performed on 13 bacterial strains isolated from preputial swabs of European bison (<jats:italic>Bison bonasus<\/jats:italic>) bulls suffering from balanoposthitis. The isolates were Gram-positive, non-motile, facultatively anaerobic, diphtheroid-shaped cells. Based on biochemical profiles and BOX-PCR-generated genomic fingerprints, the isolates were grouped into two clusters represented by four and nine strains, respectively. Strains 1(W3\/01)<jats:sup>T<\/jats:sup> and 2(W106\/04)<jats:sup>T<\/jats:sup>, selected as representatives of the two clusters, shared 97\u00b72\u200a% 16S rRNA gene sequence similarity. The highest gene sequence similarities found (95\u00b75\u201396\u00b74\u200a%) were to <jats:italic>Arcanobacterium pyogenes<\/jats:italic> DSM 20630<jats:sup>T<\/jats:sup> and <jats:italic>Arcanobacterium bernardiae<\/jats:italic> DSM 9152<jats:sup>T<\/jats:sup>, demonstrating that the novel strains are members of the genus <jats:italic>Arcanobacterium<\/jats:italic>, but are not members of a recognized species. The polar lipid profiles of the two novel strains displayed the major characteristics also found in <jats:italic>A. pyogenes<\/jats:italic> DSM 20630<jats:sup>T<\/jats:sup> and <jats:italic>Arcanobacterium haemolyticum<\/jats:italic> DSM 20595<jats:sup>T<\/jats:sup>. Detection of a quinone system with MK-10(H<jats:sub>4<\/jats:sub>) as the predominant compound confirmed phylogenetic relatedness of the novel strains to <jats:italic>A. pyogenes<\/jats:italic> and separated them from the type species of the genus, <jats:italic>A. haemolyticum<\/jats:italic>, which contains MK-9(H<jats:sub>4<\/jats:sub>) as the predominant quinone. Results from DNA\u2013DNA hybridizations clearly demonstrated that strains 1(W3\/01)<jats:sup>T<\/jats:sup> and 2(W106\/04)<jats:sup>T<\/jats:sup> represent separate species. Based on these data, two novel species of the genus <jats:italic>Arcanobacterium<\/jats:italic> are described, for which the names <jats:italic>Arcanobacterium bialowiezense<\/jats:italic> sp. nov. [type strain 1(W3\/01)<jats:sup>T<\/jats:sup>=DSM 17162<jats:sup>T<\/jats:sup>=NCTC 13354<jats:sup>T<\/jats:sup>] and <jats:italic>Arcanobacterium bonasi<\/jats:italic> sp. nov. [type strain 2(W106\/04)<jats:sup>T<\/jats:sup>=DSM 17163<jats:sup>T<\/jats:sup>=NCTC 13355<jats:sup>T<\/jats:sup>] are proposed.<\/jats:p>","DOI":"10.1099\/ijs.0.63923-0","type":"journal-article","created":{"date-parts":[[2006,4,3]],"date-time":"2006-04-03T22:23:09Z","timestamp":1144102989000},"page":"861-866","source":"Crossref","is-referenced-by-count":60,"title":["Arcanobacterium bialowiezense sp. nov. and Arcanobacterium bonasi sp. nov., isolated from the prepuce of European bison bulls (Bison bonasus) suffering from balanoposthitis, and emended description of the genus Arcanobacterium Collins et al. 1983"],"prefix":"10.1099","volume":"56","author":[{"given":"Alexandra","family":"Lehnen","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Zoo- und Wildtierforschung, Alfred-Kowalke-Str.17, D-10315 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-J\u00fcrgen","family":"Busse","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Bakteriologie, Mykologie und Hygiene, Veterin\u00e4rmedizinische Universit\u00e4t, Veterin\u00e4rplatz 1, 1210 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Fr\u00f6lich","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Zoo- und Wildtierforschung, Alfred-Kowalke-Str.17, D-10315 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malgorzata","family":"Krasinska","sequence":"additional","affiliation":[{"name":"Mammal Research Institute PAS, ul. Waszkiewicza 1c, 17-230 Bialowieza, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"K\u00e4mpfer","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Angewandte Mikrobiologie, Justus-Liebig-Universit\u00e4t, Heinrich Buff-Ring 26-32 (IFZ), D-35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Speck","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Zoo- und Wildtierforschung, Alfred-Kowalke-Str.17, D-10315 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0168-1656(96)01376-4","article-title":"Classification of bacteria isolated from a medieval wall painting","volume":"47","author":"Altenburger","year":"1996","journal-title":"J Biotechnol"},{"key":"R2","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1128\/JCM.29.1.83-89.1991","article-title":"Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic Gram-positive rods","volume":"29","author":"Bernard","year":"1991","journal-title":"J Clin Microbiol"},{"key":"R3","doi-asserted-by":"crossref","first-page":"4801","DOI":"10.1073\/pnas.75.10.4801","article-title":"Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli","volume":"75","author":"Brosius","year":"1978","journal-title":"Proc Natl Acad Sci U S A"},{"key":"R4","article-title":"Reclassification of \u2018 Corynebacterium haemolyticum \u2019 (MacLean, Liebow & Rosenberg) in the genus Arcanobacterium gen.nov. as Arcanobacterium haemolyticum nom. rev., comb. nov","author":"Collins","year":"1982"},{"key":"R5","first-page":"438","article-title":"Arcanobacterium haemolyticum nom. rev., comb. nov. 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