{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T22:10:01Z","timestamp":1748556601845,"version":"3.41.0"},"reference-count":29,"publisher":"Microbiology Society","issue":"Pt_11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,11,1]]},"abstract":"<jats:p>Two Gram-stain-positive bacterial isolates, strain 2385\/12<jats:sup>T<\/jats:sup>and strain 2673\/12<jats:sup>T<\/jats:sup>were isolated from a tapir and a dog's nose, respectively. The two strains were rod to coccoid-shaped, catalase-positive and oxidase-negative. The highest 16S rRNA gene sequence similarity identified<jats:italic>Corynebacterium singulare<\/jats:italic>CCUG 37330<jats:sup>T<\/jats:sup>(96.3\u2009% similarity) as the nearest relative of strain 2385\/12<jats:sup>T<\/jats:sup>and suggested the isolate represented a novel species.<jats:italic>Corynebacterium humireducens<\/jats:italic>DSM 45392<jats:sup>T<\/jats:sup>(98.7\u2009% 16S rRNA gene sequence similarity) was identified as the nearest relative of strain 2673\/12<jats:sup>T<\/jats:sup>. Results from DNA\u2013DNA hybridization with the type strain of<jats:italic>C. humireducens<\/jats:italic>demonstrated that strain 2673\/12<jats:sup>T<\/jats:sup>also represented a novel species. Strain 2385\/12<jats:sup>T<\/jats:sup>showed a quinone system consisting predominantly of menaquinones MK-8(H<jats:sub>2<\/jats:sub>) and MK-9(H<jats:sub>2<\/jats:sub>) whereas strain 2673\/12<jats:sup>T<\/jats:sup>contained only MK-8(H<jats:sub>2<\/jats:sub>) as predominant quinone. The polar lipid profiles of the two strains showed the major compounds phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid. Phosphatidylinositol was identified as another major lipid in 2673\/12<jats:sup>T<\/jats:sup>whereas it was only found in moderate amounts in strain 2385\/12<jats:sup>T<\/jats:sup>. Furthermore, moderate to minor amounts of phosphatidylinositol-mannoside, \u03b2-gentiobiosyl diacylglycerol and variable counts of several unidentified lipids were detected in the two strains. Both strains contained corynemycolic acids. The polyamine patterns were characterized by the major compound putrescine in strain 2385\/12<jats:sup>T<\/jats:sup>and spermidine in strain 2673\/12<jats:sup>T<\/jats:sup>. In the fatty acid profiles, predominantly C<jats:sub>18\u2009:\u20091<\/jats:sub>\u03c99<jats:italic>c<\/jats:italic>and C<jats:sub>16\u2009:\u20090<\/jats:sub>were detected. The two strains are distinguishable from each other and the nearest related established species of the genus<jats:italic>Corynebacterium<\/jats:italic>phylogenetically and phenotypically. In conclusion, two novel species of the genus<jats:italic>Corynebacterium<\/jats:italic>are proposed, namely<jats:italic>Corynebacterium tapiri<\/jats:italic>sp. nov. (type strain, 2385\/12<jats:sup>T<\/jats:sup>\u2009=\u2009CCUG 65456<jats:sup>T<\/jats:sup>\u2009=\u2009LMG 28165<jats:sup>T<\/jats:sup>) and<jats:italic>Corynebacterium nasicanis<\/jats:italic>sp. nov. (type strain, 2673\/12<jats:sup>T<\/jats:sup>\u2009=\u2009CCUG 65455<jats:sup>T<\/jats:sup>\u2009=\u2009LMG 28166<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.000510","type":"journal-article","created":{"date-parts":[[2015,8,4]],"date-time":"2015-08-04T20:12:10Z","timestamp":1438719130000},"page":"3885-3893","source":"Crossref","is-referenced-by-count":14,"title":["Corynebacterium tapiri sp. nov. and Corynebacterium nasicanis sp. nov., isolated from a tapir and a dog, respectively"],"prefix":"10.1099","volume":"65","author":[{"given":"Sandra","family":"Baumgardt","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie, Veterin\u00e4rmedizinische Universit\u00e4t Wien, A-1210 Wien, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor","family":"Loncaric","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie, Veterin\u00e4rmedizinische Universit\u00e4t Wien, A-1210 Wien, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"K\u00e4mpfer","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Angewandte Mikrobiologie, Justus-Liebig-Universit\u00e4t Giessen, D-35392, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-J\u00fcrgen","family":"Busse","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie, Veterin\u00e4rmedizinische Universit\u00e4t Wien, A-1210 Wien, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"ijsem000510-Altenburger1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0168-1656(96)01376-4","article-title":"Classification of bacteria isolated from a medieval wall painting","volume":"47","author":"Altenburger","year":"1996","journal-title":"J Biotechnol"},{"key":"ijsem000510-Altenburger12","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1099\/00207713-47-2-270","article-title":"Polyamine distribution in actinomycetes with group B peptidoglycan and species of the genera Brevibacterium, Corynebacterium, and Tsukamurella","volume":"47","author":"Altenburger","year":"1997","journal-title":"Int J Syst Bacteriol"},{"key":"ijsem000510-Bernard1","first-page":"245","article-title":"Genus I. Corynebacterium Lehmann and Neumann 1896, 350AL emend. Bernard, Wiebe Burdz, Reimer, Ng, Singh, Schindle and Pacheco 2010, 877","volume-title":"Bergey's Manual of Systematic Bacteriology","volume":"vol. 5 (The Actinobacteria)","author":"Bernard","year":"2012"},{"key":"ijsem000510-Busse1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0723-2020(88)80040-7","article-title":"Polyamine pattern as a chemotaxonomic marker within the Proteobacteria","volume":"11","author":"Busse","year":"1988","journal-title":"Syst Appl Microbiol"},{"key":"ijsem000510-Collins1","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1128\/MMBR.45.2.316-354.1981","article-title":"Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication","volume":"45","author":"Collins","year":"1981","journal-title":"Microbiol Rev"},{"key":"ijsem000510-Collins12","first-page":"129","article-title":"A survey of the structures of mycolic acids in Corynebacterium and related taxa","volume":"128","author":"Collins","year":"1982","journal-title":"J Gen Microbiol"},{"key":"ijsem000510-Frischmann1","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1099\/ijs.0.036061-0","article-title":"Corynebacterium epidermidicanis sp. nov., isolated from skin of a dog","volume":"62","author":"Frischmann","year":"2012","journal-title":"Int J Syst Evol Microbiol"},{"key":"ijsem000510-Fudou1","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1099\/00207713-52-4-1127","article-title":"Corynebacterium efficiens sp. nov., a glutamic-acid-producing species from soil and vegetables","volume":"52","author":"Fudou","year":"2002","journal-title":"Int J Syst Evol Microbiol"},{"key":"ijsem000510-Hall1","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp Ser"},{"key":"ijsem000510-Kampfer1","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1139\/m96-128","article-title":"Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa","volume":"42","author":"K\u00e4mpfer","year":"1996","journal-title":"Can J Microbiol"},{"key":"ijsem000510-Kampfer12","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/BF02539156","article-title":"Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria","volume":"21","author":"K\u00e4mpfer","year":"1991","journal-title":"Microb Ecol"},{"key":"ijsem000510-Kampfer123","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1099\/ijs.0.006379-0","article-title":"Corynebacterium lubricantis sp. nov., isolated from a coolant lubricant","volume":"59","author":"K\u00e4mpfer","year":"2009","journal-title":"Int J Syst Evol Microbiol"},{"key":"ijsem000510-Kim1","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1099\/ijs.0.038075-0","article-title":"Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species","volume":"62","author":"Kim","year":"2012","journal-title":"Int J Syst Evol Microbiol"},{"key":"ijsem000510-Lane1","first-page":"115","article-title":"16S\/23S rRNA sequencing","volume-title":"Nucleic Acid Techniques in Bacterial Systematics","author":"Lane","year":"1991"},{"key":"ijsem000510-Lee1","first-page":"2734","volume":"59","author":"Lee","year":"2009","journal-title":"Corynebacterium doosanense sp. nov., isolated from activated sludge"},{"volume-title":"Atlas und Grundriss der Bakteriologie und Lehrbuch der speciellen bakteriologischen Diagnostik","year":"1896","author":"Lehmann","key":"ijsem000510-Lehmann1"},{"key":"ijsem000510-Moaledj1","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/0167-7012(86)90056-4","article-title":"Comparison of Gram-staining and alternate methods, KOH test and aminopeptidase activity in aquatic bacteria: their application to numerical taxonomy","volume":"5","author":"Moaledj","year":"1986","journal-title":"J Microbiol Methods"},{"key":"ijsem000510-Nahaie1","first-page":"2427","article-title":"Polar lipid and isoprenoid quinone composition in the classification of Staphylococcus","volume":"130","author":"Nahaie","year":"1984","journal-title":"J Gen Microbiol"},{"key":"ijsem000510-PantUcek1","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.syapm.2012.11.004","article-title":"Staphylococcus petrasii sp. nov. including S. petrasii subsp. petrasii subsp. nov. and S. petrasii subsp. croceilyticus subsp. nov., isolated from human clinical specimens and human ear infections","volume":"36","author":"Pantu\u02da\u010dek","year":"2013","journal-title":"Syst Appl Microbiol"},{"key":"ijsem000510-Riegel1","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1099\/00207713-47-4-1092","article-title":"Corynebacterium singulare sp. nov., a new species for urease-positive strains related to Corynebacterium minutissimum","volume":"47","author":"Riegel","year":"1997","journal-title":"Int J Syst Bacteriol"},{"key":"ijsem000510-Schumann1","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/B978-0-12-387730-7.00005-X","article-title":"Peptidoglycan structure","volume":"38","author":"Schumann","year":"2011","journal-title":"Methods Microbiol"},{"key":"ijsem000510-Smibert1","first-page":"611","article-title":"Phenotypic characterization","volume-title":"Methods for General and Molecular Bacteriology","author":"Smibert","year":"1994"},{"key":"ijsem000510-Stolz1","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1099\/ijs.0.64761-0","article-title":"Pseudomonas knackmussii sp. nov","volume":"57","author":"Stolz","year":"2007","journal-title":"Int J Syst Evol Microbiol"},{"key":"ijsem000510-Tamura1","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods","volume":"28","author":"Tamura","year":"2011","journal-title":"Mol Biol Evol"},{"key":"ijsem000510-Tindall1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/j.1574-6968.1990.tb03996.x","article-title":"Lipid composition of Halobacterium lacusprofundi","volume":"66","author":"Tindall","year":"1990a","journal-title":"FEMS Microbiol Lett"},{"key":"ijsem000510-Tindall12","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/S0723-2020(11)80158-X","article-title":"A comparative study of the lipid composition of Halobacterium saccharovorum from various sources","volume":"13","author":"Tindall","year":"1990b","journal-title":"Syst Appl Microbiol"},{"key":"ijsem000510-Wu1","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1099\/ijs.0.020909-0","article-title":"Corynebacterium humireducens sp. nov., an alkaliphilic, humic acid-reducing bacterium isolated from a microbial fuel cell","volume":"61","author":"Wu","year":"2011","journal-title":"Int J Syst Evol Microbiol"},{"key":"ijsem000510-Yassin1","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1099\/ijs.0.02394-0","article-title":"Corynebacterium glaucum sp. nov","volume":"53","author":"Yassin","year":"2003","journal-title":"Int J Syst Evol Microbiol"},{"key":"ijsem000510-Ziemke1","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1099\/00207713-48-1-179","article-title":"Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov","volume":"48","author":"Ziemke","year":"1998","journal-title":"Int J Syst Bacteriol"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.000510?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T21:45:20Z","timestamp":1748555120000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.000510"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,1]]},"references-count":29,"journal-issue":{"issue":"Pt_11","published-print":{"date-parts":[[2015,11,1]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.000510","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"type":"print","value":"1466-5026"},{"type":"electronic","value":"1466-5034"}],"subject":[],"published":{"date-parts":[[2015,11,1]]}}}