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Isolates were helix-shaped, aerobic, highly motile and non-virulent in a hamster model of infection. Draft genomes of the strains were obtained and analysed to determine the relatedness to other species of the genus \n \n Leptospira<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. The analysis of 498 core genes showed that strains F1T<\/jats:sup>\/B21 and YaleT<\/jats:sup>\/AMB6-RJ formed two distinct phylogenetic clades within the \u2018Pathogens\u2019 group (group I). The average nucleotide identity (ANI) values of strains F1T<\/jats:sup>\/B21 and YaleT<\/jats:sup>\/AMB6-RJ to other previously described Leptospira<\/jats:italic> species were below <84\u200a%\u2009and <82\u200a%, respectively, which confirmed that these isolates should be classified as representatives of two novel species. Therefore, we propose \n \n Leptospira<\/jats:ext-link>\n <\/jats:named-content>yasudae<\/jats:italic> sp. nov. and \n \n Leptospira<\/jats:ext-link>\n <\/jats:named-content>stimsonii<\/jats:italic> sp. nov. as new species in the genus \n \n Leptospira<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. The type strains are F1T<\/jats:sup> (=ATCC-TSD-163=KIT0259=CLEP00287) and YaleT<\/jats:sup> (=ATCC-TDS-162=KIT0258=CLEP00288), respectively.<\/jats:p>","DOI":"10.1099\/ijsem.0.003480","type":"journal-article","created":{"date-parts":[[2019,6,11]],"date-time":"2019-06-11T14:55:27Z","timestamp":1560264927000},"page":"1450-1456","source":"Crossref","is-referenced-by-count":70,"title":["Leptospira yasudae sp. nov. and Leptospira stimsonii sp. nov., two new species of the pathogenic group isolated from environmental sources"],"prefix":"10.1099","volume":"70","author":[{"given":"Arnau","family":"Casanovas-Massana","sequence":"first","affiliation":[{"name":"Epidemiology of Microbial Diseases, School of Public Health, Yale University, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila","family":"Hamond","sequence":"additional","affiliation":[{"name":"Epidemiology of Microbial Diseases, School of Public Health, Yale University, New Haven, CT, 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