{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T03:44:58Z","timestamp":1762659898949,"version":"3.40.5"},"reference-count":41,"publisher":"Microbiology Society","issue":"5","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Special Coordination Funds of Science and Technology Department of Yunnan province and Kunming Medical University","award":["2010CD174"],"award-info":[{"award-number":["2010CD174"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"A novel nocardioform strain, CICC 11023T<\/jats:sup>, was isolated from a tissue biopsy of neck lesions of a patient with primary cutaneous nocardiosis and characterized to establish its taxonomic position. The morphological, biochemical, physiological and chemotaxonomic properties of strain CICC 11023T<\/jats:sup> were consistent with classification in the genus \n \n Nocardia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. Whole-cell hydrolysates were rich in meso-diaminopimelic acid, galactose, arabinose and fructose. Mycolic acids were present. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and two unidentified lipids, and the predominant menaquinone was cyclo MK-8 (H4, \u03c9-cyclo). The main fatty acids (>5\u200a%) were C18\u200a:\u200a0<\/jats:sub> 10-methyl (TBSA), C16\u200a:\u200a0<\/jats:sub>, summed feature 4 (C16\u200a:\u200a1<\/jats:sub> trans 9\/C15\u200a:\u200a0<\/jats:sub> iso 2OH), C15\u200a:\u200a0<\/jats:sub> and C17\u200a:\u200a0<\/jats:sub> 10-methyl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the isolate is most closely related (>98\u200a% similarity) to the type strains \n \n Nocardia ninae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> OFN 02.72T<\/jats:sup>, \n \n Nocardia iowensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> UI 122540T<\/jats:sup> and \n \n Nocardia alba<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> YIM 30243T<\/jats:sup>, and phylogenetic analysis of gyrB<\/jats:italic> gene sequences showed similarity (89.1\u201392.2\u200a%) to \n \n Nocardia vulneris<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> NBRC 108936T<\/jats:sup>, \n \n Nocardia brasiliensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> IFM 0236T<\/jats:sup> and \n \n Nocardia exalbida<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> IFM 0803T<\/jats:sup>. DNA\u2013DNA hybridization results for strain CICC 11023T<\/jats:sup> compared to \n \n Nocardia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> type strains ranged from 20.4 to 35.4\u200a%. The genome of strain CICC 11023T<\/jats:sup> was 8.78 Mbp with a G+C content of 67.4\u2009mol% overall. The average nucleotide identity (ANI) values between strain CICC 11023T<\/jats:sup> and \n \n N. alba<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> YIM 30243T<\/jats:sup> were low (OrthoANIu=77.47\u200a%), and the ANI values between strain CICC 11023T<\/jats:sup> and \n \n N. vulneris<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> NBRC 108936 T<\/jats:sup> were low (OrthoANIu=83.75 %). Consequently, strain CICC 11023T<\/jats:sup> represents a novel \n \n Nocardia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species on the basis of this polyphasic study, for which the name Nocardia colli<\/jats:italic> sp. nov. is proposed. The type strain is CICC 11023T<\/jats:sup> (=KCTC 39837T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.003856","type":"journal-article","created":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T07:36:23Z","timestamp":1588836983000},"page":"2981-2987","source":"Crossref","is-referenced-by-count":10,"title":["Nocardia colli sp. nov., a new pathogen isolated from a patient with primary cutaneous nocardiosis"],"prefix":"10.1099","volume":"70","author":[{"given":"Tao","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Medicine, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5837-4932","authenticated-orcid":false,"given":"Xiao-Yun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan-Qi","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Hua","family":"Xu","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Lan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Hua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei-Lian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Hong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-47-2-479"},{"article-title":"I Generi E Le specie delle Batteriacee. L. Zanaboni E Gabuzzi","year":"1889","author":"Trevisan","key":"R2"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.7.2.213"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02011-07"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.011346-0"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.016949-0"},{"volume-title":"In: Manual of Clinical Microbiology","year":"2011","author":"Conville","key":"R7"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2013.842553"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1159\/000321635"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.10.56"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1078\/0723-2020-00270"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1985.tb01491.x"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.039255-0"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.2323\/jgam.45.49"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-20-4-435"},{"key":"R16","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1128\/am.12.5.421-423.1964","article-title":"Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates","volume":"12","author":"Becker","year":"1964","journal-title":"Appl Microbiol"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-100-2-221"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-47-4-1129"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)88433-2"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1980.tb01036.x"},{"volume-title":"Actinobacteria - Basics and Biotechnological Applications","year":"2016","author":"Wang","key":"R21"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.6.2366-2371.2004"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.038125-0"},{"volume-title":"Methods for General and Molecular Bacteriology","year":"1994","author":"Smibert","key":"R24"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1159\/000018325"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64749-0"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.24.4876"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a040454"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/20.4.406"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1080\/10635150390235520"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm092"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-39-2-159"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1970.tb00831.x"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1988.tb01872.x"},{"volume-title":"Glimmer Release Notes Version 3.02","year":"2006","author":"Delcher","key":"R36"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.5.955"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm160"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409727102"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq461"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw569"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.003856?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T01:24:39Z","timestamp":1702603479000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.003856"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,1]]},"references-count":41,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5,1]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.003856","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"type":"print","value":"1466-5026"},{"type":"electronic","value":"1466-5034"}],"subject":[],"published":{"date-parts":[[2020,5,1]]}}}