{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T12:35:35Z","timestamp":1769171735667,"version":"3.49.0"},"reference-count":61,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,2,1]]},"abstract":"<jats:p>Three bacterial strains, HKU70<jats:sup>T<\/jats:sup>, HKU71<jats:sup>T<\/jats:sup> and HKU72<jats:sup>T<\/jats:sup>, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong. The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.6505\" xlink:type=\"simple\">Tsukamurella<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> species. 16S rRNA, <jats:italic>secA<\/jats:italic>, <jats:italic>rpoB<\/jats:italic> and <jats:italic>groEL<\/jats:italic> gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9\u200a% nucleotide identities with their corresponding closest <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.6505\" xlink:type=\"simple\">Tsukamurella<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> species respectively. DNA\u2013DNA hybridization confirmed that they were distinct from other known species of the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.6505\" xlink:type=\"simple\">Tsukamurella<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (26.2\u00b12.4 to 36.8\u00b11.2\u200a% DNA\u2013DNA relatedness), in line with results of <jats:italic>in silico<\/jats:italic> genome-to-genome comparison (32.2\u201340.9\u200a% Genome-to-Genome Distance Calculator and 86.3\u201388.9 % average nucleotide identity values]. Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.6505\" xlink:type=\"simple\">Tsukamurella<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. The G+C content determined based on the genome sequence of strains HKU70<jats:sup>T<\/jats:sup>, HKU71<jats:sup>T<\/jats:sup> and HKU72<jats:sup>T<\/jats:sup> were 69.9, 70.2 and 70.5 mol%, respectively. Taken together, our results supported the proposition and description of three new species, i.e. <jats:italic>Tsukamurella sputi<\/jats:italic> HKU70<jats:sup>T<\/jats:sup> (=JCM 33387<jats:sup>T<\/jats:sup>=DSM 109106<jats:sup>T<\/jats:sup>) sp. nov., <jats:italic>Tsukamurella asaccharolytica<\/jats:italic> HKU71<jats:sup>T<\/jats:sup> (=JCM 33388<jats:sup>T<\/jats:sup>=DSM 109107<jats:sup>T<\/jats:sup>) sp. nov. and <jats:italic>Tsukamurella conjunctivitidis<\/jats:italic> HKU72<jats:sup>T<\/jats:sup> (=JCM 33389<jats:sup>T<\/jats:sup>=DSM 109108<jats:sup>T<\/jats:sup>) sp. nov.<\/jats:p>","DOI":"10.1099\/ijsem.0.003861","type":"journal-article","created":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T17:12:25Z","timestamp":1574097145000},"page":"995-1006","source":"Crossref","is-referenced-by-count":27,"title":["Tsukamurella asaccharolytica sp. nov., Tsukamurella conjunctivitidis sp. nov. and Tsukamurella sputi sp. nov., isolated from patients with bacteraemia, conjunctivitis and respiratory infection in Hong Kong"],"prefix":"10.1099","volume":"70","author":[{"given":"Jade L. 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