{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:46:28Z","timestamp":1791917188469,"version":"4.3.4"},"reference-count":77,"publisher":"Microbiology Society","issue":"2","funder":[{"name":"Russian Foundation for Basic Research","award":["17-04-01578"],"award-info":[{"award-number":["17-04-01578"]}]},{"name":"Ministry of Science and Higher Education within the State assignment FRC \u201cFundamentals of Biotechnology\u201d RAS"},{"name":"Scholarship of the President of the Russian Federation","award":["SP-2728.2019.4."],"award-info":[{"award-number":["SP-2728.2019.4."]}]},{"name":"the President of Russia","award":["\u041c\u041a-3801.2018.4"],"award-info":[{"award-number":["\u041c\u041a-3801.2018.4"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,2,1]]},"abstract":"<jats:p>\n                    A novel aerobic moderately thermophilic bacterium, strain 3753O\n                    <jats:sup>T<\/jats:sup>\n                    , was isolated from a Chukotka hot spring (Arctic, Russia) using the newly developed technology of laser engineering of microbial systems. \u0421ells were regular short rods, 0.4\u00d70.8\u20132.0\u2009\u00b5m in size, with a monoderm-type envelope and a single flagellum. The temperature and pH ranges for growth were 42\u201360\u2009\u00b0C\u2009and pH 6.5\u20138.5, the optima being 50\u201354\u2009\u00b0C\u2009and pH 7.3. Strain 3753O\n                    <jats:sup>T<\/jats:sup>\n                    grew chemoorganoheterotrophically on a number of carbohydrates or peptidic substrates and volatile fatty acids, and chemolithoautotrophically with siderite (FeCO\n                    <jats:sub>3<\/jats:sub>\n                    ) as the electron donor. The major cellular fatty acid was branched C\n                    <jats:sub>19\u200a:\u200a0<\/jats:sub>\n                    . Phosphatidylethanolamine, phosphatidylglycerol and two unidentified phospholipids as well as two yellow carotenoid-type pigments were detected in the polar lipid extract. Strain 3753O\n                    <jats:sup>T<\/jats:sup>\n                    was inhibited by chloramphenicol, polymyxin B, vancomycin, streptomycin, neomycin and kanamycin, but resistant to the action of novobiocin and ampicillin. The DNA G+C content was 69.9\u2009mol%. The 16S rRNA gene as well as 51 conservative protein sequence-based phylogenetic analyses placed strain 3753O\n                    <jats:sup>T<\/jats:sup>\n                    within the previously uncultivated lineage OLB14 in the phylum\n                    <jats:italic>\n                      <jats:named-content content-type=\"class\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.550\" xlink:type=\"simple\">Chloroflexi<\/jats:ext-link>\n                      <\/jats:named-content>\n                    <\/jats:italic>\n                    . Taking into account the phylogenetic position as well as phenotypic properties of the novel isolate, the novel genus and species\n                    <jats:italic>Tepidiforma bonchosmolovskayae<\/jats:italic>\n                    gen. nov.\n                    <jats:italic>,<\/jats:italic>\n                    sp. nov., within the\n                    <jats:italic>Tepidiformaceae<\/jats:italic>\n                    fam. nov., the\n                    <jats:italic>Tepidiformales<\/jats:italic>\n                    ord. nov. and the\n                    <jats:italic>Tepidiformia<\/jats:italic>\n                    classis nov. are proposed. The type strain of\n                    <jats:italic>Tepidiforma bonchosmolovskayae<\/jats:italic>\n                    is 3753O\n                    <jats:sup>T<\/jats:sup>\n                    (=VKM B-3389\n                    <jats:sup>T<\/jats:sup>\n                    =KTCT 72284\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.003902","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T10:46:39Z","timestamp":1574765199000},"page":"1192-1202","source":"Crossref","is-referenced-by-count":61,"title":["Tepidiforma bonchosmolovskayae gen. nov., sp. nov., a moderately thermophilic Chloroflexi bacterium from a Chukotka hot spring (Arctic, Russia), representing a novel class, Tepidiformia, which includes the previously uncultivated lineage OLB14"],"prefix":"10.1099","volume":"70","author":[{"given":"Tatiana 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