{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T04:06:05Z","timestamp":1768449965655,"version":"3.49.0"},"reference-count":19,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,1]]},"abstract":"Five strains of an unidentified Gram-positive, catalase-negative, chain-forming coccus-shaped organism recovered from sheep in Scotland were characterized using phenotypic and molecular taxonomic methods. Based on morphological and biochemical criteria, the strains were tentatively identified as streptococci but they did not appear to correspond to any recognised species of the genus. Comparative 16S rRNA gene sequencing showed the strains were highly related to each other and confirmed their placement in the genus \n \n Streptococcus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, with a maximum nucleotide identity of around 97\u200a% to extant species. Best matches were with \n \n Streptococcus hillyeri<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> followed by \n \n Streptococcus porci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. Average nucleotide identity and in silico<\/jats:italic> DNA\u2013DNA hybridization values determined from whole-genome sequence were also consistent with the group representing a novel species. Best matches, again seen to \n \n S. hillyeri<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, followed by \n \n S. porci<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n S. plurextorum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, were below accepted cut-off values for species delineation. Based on biochemical criteria and molecular genetic evidence, it is proposed that the unknown isolates from sheep be assigned to a new species of the genus \n \n Streptococcus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> as Streptococcus caledonicus<\/jats:italic> sp. nov. The type strain of Streptococcus caledonicus<\/jats:italic> is S784\/96\/1T<\/jats:sup>=CCUG 73951T<\/jats:sup>=NCTC 14363T<\/jats:sup>.<\/jats:p>","DOI":"10.1099\/ijsem.0.004081","type":"journal-article","created":{"date-parts":[[2020,3,11]],"date-time":"2020-03-11T16:17:08Z","timestamp":1583943428000},"page":"2611-2615","source":"Crossref","is-referenced-by-count":13,"title":["Streptococcus caledonicus sp. nov., isolated from sheep"],"prefix":"10.1099","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5527-758X","authenticated-orcid":false,"given":"Geoffrey","family":"Foster","sequence":"first","affiliation":[{"name":"SRUC Veterinary Services, An Lochran, 10 Inverness Campus, Inverness IV2 5NA, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2468-0058","authenticated-orcid":false,"given":"Miranda","family":"Kirchner","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jakub","family":"Muchowski","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Duggett","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luke","family":"Randall","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7660-7728","authenticated-orcid":false,"given":"Hazel I.","family":"Knight","sequence":"additional","affiliation":[{"name":"SRUC Veterinary Services, Mill of Craibstone, Bucksburn, Aberdeen AB21 9TB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4218-156X","authenticated-orcid":false,"given":"Adrian M.","family":"Whatmore","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Animal and Plant Health Agency, Addlestone, KT15 3NB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","first-page":"655","volume-title":"Bergey\u2019s Manual of Systematic Bacteriology. 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