{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T08:24:13Z","timestamp":1789287853108,"version":"build-2803163510"},"reference-count":31,"publisher":"Microbiology Society","issue":"4","funder":[{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Yakumo Foundation"},{"name":"KAKENHI","award":["18H02129"],"award-info":[{"award-number":["18H02129"]}]},{"name":"KAKENHI","award":["15H04476"],"award-info":[{"award-number":["15H04476"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,1]]},"abstract":"<jats:p>A new lanthanide (Ln<jats:sup>3+<\/jats:sup>)-dependent methanol-utilizing bacterial strain, La3113<jats:sup>T<\/jats:sup>, was isolated from rice field soil and its taxonomic position was investigated using polyphasic approaches. The strain was aerobic, Gram-stain-negative, strongly motile, catalase-positive and cytochrome oxidase-positive. It could neither catalyse the hydrolysis of urea nor reduce nitrate to nitrite. Growth was observed within a temperature range of 10\u201340\u2009\u00b0C\u2009and a pH range of 6\u20138, with optimum growth at 28\u2009\u00b0C and pH 7. Methylamine was utilized as the single source of energy, carbon and nitrogen, and it was oxidized by methylamine dehydrogenase. C<jats:sub>16\u200a:\u200a1<\/jats:sub>\n                  <jats:italic>\u2009\u03c9<\/jats:italic>7<jats:italic>c<\/jats:italic>, C<jats:sub>16\u200a:\u200a1<\/jats:sub>\n                  <jats:italic>\u2009\u03c9<\/jats:italic>6<jats:italic>c<\/jats:italic> and C<jats:sub>16\u200a:\u200a0<\/jats:sub> were the dominant cellular fatty acids. Its draft genome (2.67 Mbp and 44.9\u2009mol% G+C content) encodes genes including three Ln<jats:sup>3+<\/jats:sup>-dependent methanol dehydrogenase (XoxF-type MDH) genes, those for formaldehyde assimilation (ribulose monophosphate pathway), formate dehydrogenases and methylamine dehydrogenases, but not Ca<jats:sup>2+<\/jats:sup>-dependent MDH (MxaFI-MDH), which characterizes the species as a Ln<jats:sup>3+<\/jats:sup>-dependent methylotroph. The 16S rRNA gene sequence showed that strain La3113<jats:sup>T<\/jats:sup> belongs to the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10406\" xlink:type=\"simple\">Methylotenera<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> and is closely related to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10864\" xlink:type=\"simple\">Methylotenera mobilis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> JLW8<jats:sup>T<\/jats:sup> (98.29\u200a% identity). The digital DNA\u2013DNA hybridization (dDDH) values (less than 30 %) and average nucleotide identity (ANI) values (less than 85\u200a%) between genomes of strain La3113<jats:sup>T<\/jats:sup> and related type strains were lower than the thresholds for species delineation (70\u200a% for dDDH and 95\u201396\u200a% for ANI). On the basis of these polyphasic approaches, we propose a novel <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10406\" xlink:type=\"simple\">Methylotenera<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> species, <jats:italic>Methylotenera oryzisoli<\/jats:italic> sp. nov. (type strain La3113<jats:sup>T<\/jats:sup>=NBRC 111954<jats:sup>T<\/jats:sup>=DSM 103219<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.004098","type":"journal-article","created":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T18:16:33Z","timestamp":1584382593000},"page":"2713-2718","source":"Crossref","is-referenced-by-count":26,"title":["Methylotenera oryzisoli sp. nov., a lanthanide-dependent methylotrophic bacteria isolated from rice field soil"],"prefix":"10.1099","volume":"70","author":[{"given":"Haoxin","family":"Lv","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Henan University of Technology, Zhengzhou, PR China"},{"name":"Institute of Plant Science and Resources, Okayama University, Okayama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1049-6180","authenticated-orcid":false,"given":"Nurettin","family":"Sahin","sequence":"additional","affiliation":[{"name":"Egitim Fakultesi, Mugla Sitki Kocman University, Kotekli, Mugla, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7685-9063","authenticated-orcid":false,"given":"Akio","family":"Tani","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources, Okayama University, Okayama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","volume-title":"The Biochemistry of Methylotrophs","author":"Anthony","year":"1982"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-5766-8"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2010.12.017"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02464.x"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12896"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiv105"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-016-0808-7"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1264\/jsme2.ME17070"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001660"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14062"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64191-0"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.029165-0"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0102458"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-61-2-205"},{"key":"R15","volume-title":"Methods for General and Molecular Bacteriology","author":"Gerhardt","year":"1994"},{"key":"R16","first-page":"607","volume-title":"Methods for General and Molecular Bacteriology","author":"Smibert","year":"1994"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-1-17"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63405-0"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040784"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw569"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1068"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.000760"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-60"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906412106"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001755"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm864"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a040454"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01511-14"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-018-8014-6"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12896"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.004098?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T10:18:10Z","timestamp":1606990690000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.004098"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,1]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,4,1]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.004098","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,1]]}}}