{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T09:34:57Z","timestamp":1770975297245,"version":"3.50.1"},"reference-count":37,"publisher":"Microbiology Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,1]]},"abstract":"<jats:p>A moderately thermophilic, neutrophilic, aerobic, Gram-negative bacterium, strain 3729k<jats:sup>T<\/jats:sup>, was isolated from a thermal spring of the Chukotka Peninsula, Arctic region, Russia. It grew chemoorganoheterotrophically, utilizing proteinaceous substrates, including highly rigid keratins as well as various polysaccharides (glucomannan, locust bean gum, gum guar and xanthan gum). The major fatty acids of strain 3729k<jats:sup>T<\/jats:sup>were iso-C<jats:sub>15\u200a:\u200a0<\/jats:sub>(60.9%), iso-C<jats:sub>17\u200a:\u200a0<\/jats:sub>(12.0%), C<jats:sub>16\u200a:\u200a0<\/jats:sub>(9.9%) and iso-C<jats:sub>16\u200a:\u200a0<\/jats:sub>(7.4%). Isoprenoid quinones were Q-8 (95%) and Q-9 (5%). The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine and three unidentified polar lipids. Strain 3729k<jats:sup>T<\/jats:sup>was inhibited by chloramphenicol, neomycin, novobiocin, kanamycin, tetracycline, ampicillin and polymyxin B, but resistant to rifampicin, vancomycin and streptomycin. At the same time, strain 3729k<jats:sup>T<\/jats:sup>inhibited growth of<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5829\" xlink:type=\"simple\">Micrococcus luteus<\/jats:ext-link><\/jats:named-content><\/jats:italic>and its genome possessed genes for antimicrobial activity against Gram-positive bacteria (a single putative bacteriocin and several secreted lysozymes and peptidoglycan lytic transglycosylases). The DNA G+C\u2009content was 69.8\u2009mol%. 16S rRNA gene sequence-based phylogenetic analysis placed strain 3729k<jats:sup>T<\/jats:sup>into a distinct species\/genus-level branch within the family<jats:italic><jats:named-content content-type=\"family\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2207\" xlink:type=\"simple\">Xanthomonadaceae<\/jats:ext-link><\/jats:named-content><\/jats:italic>(<jats:italic><jats:named-content content-type=\"phylum\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.808\" xlink:type=\"simple\">Proteobacteria<\/jats:ext-link><\/jats:named-content><\/jats:italic>). Phylogenetic analysis of 120 conservative protein sequences of all<jats:italic><jats:named-content content-type=\"family\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.2207\" xlink:type=\"simple\">Xanthomonadaceae<\/jats:ext-link><\/jats:named-content><\/jats:italic>with validly published names and publicly available genomic sequences supported a species-level position of strain 3729k<jats:sup>T<\/jats:sup>within the genus<jats:italic><jats:named-content content-type=\"genus\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10359\" xlink:type=\"simple\">Arenimonas<\/jats:ext-link><\/jats:named-content><\/jats:italic>. Pairwise ANI values between strain 3729k<jats:sup>T<\/jats:sup>and other<jats:italic><jats:named-content content-type=\"genus\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.10359\" xlink:type=\"simple\">Arenimonas<\/jats:ext-link><\/jats:named-content><\/jats:italic>species were of 75\u201380\u200a%, supporting the proposal of a novel species. Accordingly,<jats:italic>Arenimonas fontis<\/jats:italic>sp. nov., with the type strain 3729k<jats:sup>T<\/jats:sup>(=VMK \u0412\u22123232<jats:sup>\u0422<\/jats:sup>=DSM 105847<jats:sup>T<\/jats:sup>), was proposed.<\/jats:p>","DOI":"10.1099\/ijsem.0.004099","type":"journal-article","created":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T18:16:57Z","timestamp":1584382617000},"page":"2726-2731","source":"Crossref","is-referenced-by-count":14,"title":["Arenimonas fontis sp. nov., a bacterium isolated from Chukotka hot spring, Arctic region, Russia"],"prefix":"10.1099","volume":"70","author":[{"given":"Kseniya S.","family":"Zayulina","sequence":"first","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Centre of Biotechnology of the Russian Academy of Sciences, 117312, prospect 60-Letya Oktyabrya 7\/2, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6555-2397","authenticated-orcid":false,"given":"Maria I.","family":"Prokofeva","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Centre of Biotechnology of the Russian Academy of Sciences, 117312, prospect 60-Letya Oktyabrya 7\/2, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander G.","family":"Elcheninov","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Centre of Biotechnology of the Russian Academy of Sciences, 117312, prospect 60-Letya Oktyabrya 7\/2, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5501-0316","authenticated-orcid":false,"given":"Margarita P.","family":"Voytova","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Centre of Biotechnology of the Russian Academy of Sciences, 117312, prospect 60-Letya Oktyabrya 7\/2, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0887-6678","authenticated-orcid":false,"given":"Andrei A.","family":"Novikov","sequence":"additional","affiliation":[{"name":"Gubkin University, 119991, Leninsky prospect, 65-1, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatiana V.","family":"Kochetkova","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Centre of Biotechnology of the Russian Academy of Sciences, 117312, prospect 60-Letya Oktyabrya 7\/2, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilya V.","family":"Kublanov","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Centre of Biotechnology of the Russian Academy of Sciences, 117312, prospect 60-Letya Oktyabrya 7\/2, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64457-0"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.006031-0"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-015-0409-3"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64975-0"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.002801"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-017-0876-9"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.002026"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.003785"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-012-1301-8"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.067769-0"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.000201"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.035410-0"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.000913"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.034132-0"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64472-0"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.034868-0"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.002937"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.003902"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.063172-0"},{"key":"R20","doi-asserted-by":"crossref","first-page":"2882","DOI":"10.1016\/S0021-9258(18)67912-8","article-title":"Formation of methane by bacterial extracts","volume":"238","author":"Wolin","year":"1963","journal-title":"J Biol Chem"},{"key":"R21","first-page":"563","article-title":"The influence of sulfur compounds on the growth of halophilic homoacetic bacterium Acetohalobium arabaticum","volume":"61","author":"Kevbrin","year":"1992","journal-title":"Microbiology"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1007\/s00792-013-0564-2"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001745"},{"key":"R24","first-page":"3","volume-title":"Methods in Molecular Biology, 2007, ver. 353: Protocols for Nucleic Acid Analysis by Nonradioactive Probes","author":"Park","year":"2007"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbx108"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw054"},{"key":"R27","volume-title":"Phylogenetics","author":"Nei","year":"2000"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4229"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026334"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009490"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz239"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1039\/C5AY02550H"},{"key":"R33","article-title":"The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes","author":"Rodriguez-R","year":"2016","journal-title":"PeerJ Prepr"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh454"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1007\/s007920100239"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz310"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky418"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.004099?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,4]],"date-time":"2021-03-04T14:36:12Z","timestamp":1614868572000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.004099"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,1]]},"references-count":37,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,4,1]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.004099","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,1]]}}}