{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T07:14:39Z","timestamp":1767597279405,"version":"3.40.5"},"reference-count":32,"publisher":"Microbiology Society","issue":"4","funder":[{"name":"the Key Realm R&D Program of Guangdong Province","award":["2018B020205001"],"award-info":[{"award-number":["2018B020205001"]}]},{"name":"the Science and Technology Programs of Guangdong Province","award":["2018B030324001"],"award-info":[{"award-number":["2018B030324001"]}]},{"name":"the GDAS' Special Project of Science and Technology Development","award":["2020GDASYL-20200302002"],"award-info":[{"award-number":["2020GDASYL-20200302002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,1]]},"abstract":"<jats:p>A novel pink-pigmented strain, designated 6HR-1<jats:sup>T<\/jats:sup>, was isolated from tungsten mine tailings in Jiangxi Province, PR China. Cells were Gram-stain-negative, aerobic, non-spore-forming, rod-shaped and motile with a polar flagellum (monotrichous). It could not utilize methanol, methylamine, formaldehyde or formate as a sole carbon source. The methanol dehydrogenase<jats:italic>mxaF<\/jats:italic>gene was absent but the<jats:italic>xoxF<\/jats:italic>gene was present. Phylogenomic and 16S rRNA gene phylogenetic analyses clearly showed that strain 6HR-1<jats:sup>T<\/jats:sup>was affiliated to the genus<jats:italic><jats:named-content content-type=\"genus\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1581\" xlink:type=\"simple\">Methylobacterium<\/jats:ext-link><\/jats:named-content><\/jats:italic>and closely related to \u2018<jats:italic>Methylobacterium terrae<\/jats:italic>\u2019 17Sr1-28<jats:sup>T<\/jats:sup>(98.6 %),<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.11566\" xlink:type=\"simple\">Methylobacterium platani<\/jats:ext-link><\/jats:named-content><\/jats:italic>JCM 14648<jats:sup>T<\/jats:sup>(97.7 %),<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.9518\" xlink:type=\"simple\">Methylobacterium variabile<\/jats:ext-link><\/jats:named-content><\/jats:italic>DSM 16961<jats:sup>T<\/jats:sup>(97.7 %) and<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.33092\" xlink:type=\"simple\">Methylobacterium currus<\/jats:ext-link><\/jats:named-content><\/jats:italic>KACC 19662<jats:sup>T<\/jats:sup>(97.4 %). The average nucleotide identity and digital DNA\u2013DNA hybridization values between strain 6HR-1<jats:sup>T<\/jats:sup>and its closely related type species were 87.4\u201388.7 and 33.2\u201336.3 %, respectively. It had summed feature 8 (C<jats:sub>18 : 1<\/jats:sub>\u03c97<jats:italic>c<\/jats:italic>and\/or C<jats:sub>18 : 1<\/jats:sub>\u03c96<jats:italic>c<\/jats:italic>) as the major fatty acid and ubiquinone 10 as the predominant respiratory quinone. Polyphasic characterization supported that strain 6HR-1<jats:sup>T<\/jats:sup>represents a novel species of the genus<jats:italic><jats:named-content content-type=\"genus\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.1581\" xlink:type=\"simple\">Methylobacterium<\/jats:ext-link><\/jats:named-content><\/jats:italic>, for which the name<jats:italic>Methylobacterium nonmethylotrophicum<\/jats:italic>sp. nov. is proposed with the type strain 6HR-1<jats:sup>T<\/jats:sup>(=GDMCC 1.662<jats:sup>T<\/jats:sup>=KCTC 42760<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.004112","type":"journal-article","created":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T15:57:52Z","timestamp":1585065472000},"page":"2867-2872","source":"Crossref","is-referenced-by-count":19,"title":["Methylobacterium nonmethylotrophicum sp. nov., isolated from tungsten mine tailing"],"prefix":"10.1099","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2517-538X","authenticated-orcid":false,"given":"Guang-Da","family":"Feng","sequence":"first","affiliation":[{"name":"State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8777-3537","authenticated-orcid":false,"given":"Wendi","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 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