{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T03:52:03Z","timestamp":1759117923376,"version":"3.40.5"},"reference-count":30,"publisher":"Microbiology Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,1]]},"abstract":"<jats:p>Twenty-nine isolates of an unknown haemophilic organism were isolated from the lungs of pigs from 14 farms in Australia. Phylogenetic analyses based on the 16S rRNA gene,<jats:italic>recN<\/jats:italic>and<jats:italic>rpoA<\/jats:italic>showed a monophyletic group that was most closely related to<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.36042\" xlink:type=\"simple\">Glaesserella parasuis<\/jats:ext-link><\/jats:named-content><\/jats:italic>and [<jats:italic><jats:named-content content-type=\"genus\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3396\" xlink:type=\"simple\">Actinobacillus<\/jats:ext-link><\/jats:named-content><\/jats:italic>]<jats:italic>indolicus<\/jats:italic>. Whole genome sequence analysis indicated that the<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.36042\" xlink:type=\"simple\">Glaesserella parasuis<\/jats:ext-link><\/jats:named-content><\/jats:italic>and this group, using the type strain HS4635<jats:sup>T<\/jats:sup>for comparison, showed a similarity of 30.9\u200a% DNA\u2013DNA renaturation. The isolates were Gram-stain-negative, NAD-dependent, CAMP-negative and were oxidase-positive, catalase-negative and produced indole but not urease. The isolates could be separated from all currently recognized haemophilic and non-haemophilic members of the family<jats:italic>Pastuerellaceae<\/jats:italic>. Key phenotypic properties were the production of indole, the lack of urease activity, production of \u03b2-galactosidase but not \u03b1-fucosidase, acid formation from (\u2212)-<jats:sc>d<\/jats:sc>-arabinose, (+)-<jats:sc>d<\/jats:sc>-galactose, maltose and trehalose and a failure to produce acid from (\u2212)-<jats:sc>d<\/jats:sc>-mannitol. Taken together, these data indicate that the isolates belong to a novel species for which the name<jats:italic>Glaesserella australis<\/jats:italic>sp. nov. is proposed. The type strain is HS4635<jats:sup>T<\/jats:sup>(=CCUG 71931<jats:sup>T<\/jats:sup>and LMG 30645<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.004221","type":"journal-article","created":{"date-parts":[[2020,5,17]],"date-time":"2020-05-17T07:14:05Z","timestamp":1589699645000},"page":"3686-3692","source":"Crossref","is-referenced-by-count":17,"title":["Glaesserella australis sp. nov., isolated from the lungs of pigs"],"prefix":"10.1099","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0387-8535","authenticated-orcid":false,"given":"Conny","family":"Turni","sequence":"first","affiliation":[{"name":"Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, Queensland 4067, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6268-5151","authenticated-orcid":false,"given":"Yunchen","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4067, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3802-6703","authenticated-orcid":false,"given":"Lida","family":"Omaleki","sequence":"additional","affiliation":[{"name":"Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, Queensland 4067, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nhan","family":"Giang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4067, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4107-9451","authenticated-orcid":false,"given":"P.J.","family":"Blackall","sequence":"additional","affiliation":[{"name":"Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, Queensland 4067, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4238-4658","authenticated-orcid":false,"given":"Henrik","family":"Christensen","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Copenhagen, 4 Stigb\u00f8jlen, DK-1870 Frederiksberg C, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1007\/978-3-642-38922-1_224","volume-title":"The Prokaryotes","volume":"9","author":"Christensen","year":"2014"},{"key":"R2","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1002\/9781119350927.ch48","volume-title":"Diseases of Swine","author":"Gottschalk","year":"2019"},{"key":"R3","article-title":"Phylogenomic analysis of Haemophilus parasuis and proposed reclassification to Glaesserella parasuis, gen. nov., comb. nov","author":"Dickerman","year":"2019","journal-title":"Int J Sys Evol Microbiol"},{"key":"R4","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1002\/9781119350927.ch54","volume-title":"Diseases of Swine","author":"Aragon","year":"2019"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-4-951"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0450.2001.00433.x"},{"key":"R7","first-page":"7","article-title":"Cultural and biochemical criteria for the identification of Haemophilus spp from swine","volume":"38","author":"Biberstein","year":"1977","journal-title":"Am J Vet Res"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.09.030"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00341-3"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2007.04.001"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1136\/vr.159.19.642-a"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2006.11.016"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2006.10.008"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1177\/104063870101300607"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28991-0"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"R17","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucl Acids Symp Ser"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(82)90016-5"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7012(83)90042-8"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-44-4-846"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.4056\/sigs.531120"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.4056\/sigs.541628"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-37-4-463"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.60.5.2139-2142.1992"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/5261719"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-8-1723"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00092-18"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.28.12.2711-2716.1990"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.01.017"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.004221?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T22:41:53Z","timestamp":1722897713000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.004221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,1]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,6,1]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.004221","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"type":"print","value":"1466-5026"},{"type":"electronic","value":"1466-5034"}],"subject":[],"published":{"date-parts":[[2020,6,1]]}}}