{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T01:57:27Z","timestamp":1770602247204,"version":"3.49.0"},"reference-count":39,"publisher":"Microbiology Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:p>Bacterial strain CCP-6<jats:sup>T<\/jats:sup>, isolated from a freshwater pond in Taiwan, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain CCP-6<jats:sup>T<\/jats:sup>is affiliated with species in the genus<jats:italic><jats:named-content content-type=\"genus\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.8814\" xlink:type=\"simple\">Rhodovarius<\/jats:ext-link><\/jats:named-content><\/jats:italic>. Strain CCP-6<jats:sup>T<\/jats:sup>was most closely related to<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.8815\" xlink:type=\"simple\">Rhodovarius lipocyclicus<\/jats:ext-link><\/jats:named-content><\/jats:italic>CCUG 44693<jats:sup>T<\/jats:sup>with a 98.9% 16S rRNA gene sequence similarity. Cells were Gram-stain-negative, aerobic, non-motile, rod-shaped and formed light pink-coloured colonies. Optimal growth occurred at 30 \u00b0C, pH 6 and in the absence of NaCl. The major fatty acids of strain CCP-6<jats:sup>T<\/jats:sup>were C<jats:sub>18 : 1<\/jats:sub><jats:italic>\u03c9<\/jats:italic>7<jats:italic>c<\/jats:italic>, C<jats:sub>16 : 0<\/jats:sub>and C<jats:sub>19 : 0<\/jats:sub>cyclo<jats:italic>\u03c9<\/jats:italic>8<jats:italic>c<\/jats:italic>. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidyldimethylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, three unidentified aminophospholipids and an unidentified phospholipid. The predominant polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content of the genomic DNA was 69.3 mol%. Strain CCP-6<jats:sup>T<\/jats:sup>showed 85.8% average nucleotide identity and 14.5% digital DNA\u2013DNA hybridization identity with<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.8815\" xlink:type=\"simple\">Rhodovarius lipocyclicus<\/jats:ext-link><\/jats:named-content><\/jats:italic>CCUG 44693<jats:sup>T<\/jats:sup>. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain CCP-6<jats:sup>T<\/jats:sup>represents a novel species in the genus<jats:italic><jats:named-content content-type=\"genus\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.8814\" xlink:type=\"simple\">Rhodovarius<\/jats:ext-link><\/jats:named-content><\/jats:italic>, for which the name<jats:italic>Rhodovarius crocodyli<\/jats:italic>sp. nov. is proposed. The type strain is CCP-6<jats:sup>T<\/jats:sup>(=BCRC 81095<jats:sup>T<\/jats:sup>=LMG 30310<jats:sup>T<\/jats:sup>=KCTC 62188<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.004396","type":"journal-article","created":{"date-parts":[[2020,8,19]],"date-time":"2020-08-19T15:16:59Z","timestamp":1597850219000},"page":"5141-5148","source":"Crossref","is-referenced-by-count":10,"title":["Rhodovarius crocodyli sp. nov., isolated from a crocodile pond"],"prefix":"10.1099","volume":"70","author":[{"given":"Wen-Ming","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Ru","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Der-Shyan","family":"Sheu","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung City 811, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyh-Ming","family":"Tsai","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung City 811, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shih-Yi","family":"Sheu","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung City 811, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1078\/0723202041748235"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.173.2.697-703.1991"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-47-2-249"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001755"},{"key":"R5","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.0030056","article-title":"Query-dependent banding (QDB) for faster RNA similarity searches","volume":"3","author":"Nawrocki","year":"2007","journal-title":"PLoS Comput Biol"},{"key":"R6","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp Ser"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw054"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a040454"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1007\/BF01734359"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.2307\/2412407"},{"key":"R11","doi-asserted-by":"crossref","DOI":"10.1017\/CBO9780511623486","volume-title":"The Neutral Theory of Molecular Evolution","author":"Kimura","year":"1983"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a040023"},{"key":"R13","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780195135848.001.0001","volume-title":"Molecular Evolution and Phylogenetics","author":"Nei","year":"2000"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw354"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.33"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.000760"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-60"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906412106"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64483-0"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-018-8014-6"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1226"},{"key":"R25","first-page":"19","volume-title":"Sampling and Staining for Light Microscopy","author":"Beveridge","year":"2007"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1007\/BF00410161"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050681"},{"key":"R28","first-page":"403","volume-title":"Analysis of Pigments: Carotenoids and Related Polyenes","author":"Schmidt","year":"1994"},{"key":"R29","first-page":"309","volume-title":"Physicochemical Factors in Growth","author":"Breznak","year":"2007"},{"key":"R30","first-page":"330","volume-title":"Phenotypic Characterization and the Principles of Comparative Systematic","author":"Tindall","year":"2007"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1042\/BA20020001"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-50-5-1861"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1078\/0723-2020-00252"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-33-1-26"},{"key":"R35","volume-title":"Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids","author":"Sasser","year":"1990"},{"key":"R36","first-page":"121","volume-title":"Structural Lipids of Eubacteria","author":"Embley","year":"1994"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(88)80040-7"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-47-3-698"},{"key":"R39","first-page":"265","volume-title":"Isoprenoid Quinones","author":"Collins","year":"1994"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.004396?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T03:04:55Z","timestamp":1723431895000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.004396"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,1]]},"references-count":39,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,9,1]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.004396","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,1]]}}}