{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:36:14Z","timestamp":1772156174439,"version":"3.50.1"},"reference-count":102,"publisher":"Microbiology Society","issue":"3","funder":[{"DOI":"10.13039\/501100004836","name":"Det Frie Forskningsr\u00e5d","doi-asserted-by":"crossref","award":["DFF-6111-00316"],"award-info":[{"award-number":["DFF-6111-00316"]}],"id":[{"id":"10.13039\/501100004836","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,6,6]]},"abstract":"\n A bacterial strain, designated WCA-9-b2\n T<\/jats:sup>\n , was isolated from the caecal content of an 18-week-old obese C57BL\/6NTac male mouse. According to phenotypic analyses, the isolate was rod-shaped, strictly anaerobic, spore-forming, non-motile and Gram-stain-positive, under the conditions tested. Colonies were irregular and non-pigmented. Analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the order\n \n \n Clostridiales<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n with\n \n \n Dorea longicatena<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ATCC 27755\n T<\/jats:sup>\n (94.9\u200a% sequence identity),\n \n \n Ruminococcus gnavus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ATCC 29149\n T<\/jats:sup>\n (94.8%) and\n \n \n Clostridium scindens<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ATCC 35704\n T<\/jats:sup>\n (94.3%) being the closest relatives. Whole genome sequencing showed an average nucleotide identity <74.23 %, average amino acid identity <64.52\u201374.67\u200a% and percentage of conserved proteins values <50\u200a% against the nine closest relatives (\n \n \n D. longicatena<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n Ruminococcus gnavus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n C. scindens<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n Dorea formicigenerans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n Ruminococcus lactaris<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n Clostridium hylemonae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n Merdimonas faecis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ,\n \n \n Faecalicatena contorta<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n and\n \n \n Faecalicatena fissicatena<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n ). The genome-based G+C content of genomic DNA was 44.4\u2009mol%. The major cellular fatty acids were C\n 16\u200a:\u200a0<\/jats:sub>\n (24.5%), C\n 18\u200a:\u200a1<\/jats:sub>\n cis<\/jats:italic>\n 9 (19.8 %), C\n 16\u200a:\u200a0<\/jats:sub>\n DMA (11.7%), C\n 18\u200a:\u200a0<\/jats:sub>\n (8.4%) and C\n 14\u200a:\u200a0<\/jats:sub>\n (6.6%). Respiratory quinones were not detected. The predominant metabolic end products of glucose fermentation were acetate and succinate. Production of CO\n 2<\/jats:sub>\n and H\n 2<\/jats:sub>\n were detected. Based on these data, we propose that strain WCA-9-b2\n T<\/jats:sup>\n represents a novel species within a novel genus, for which the name\n Sporofaciens musculi<\/jats:italic>\n gen. nov., sp. nov. is proposed. The type strain is WCA-9-b2\n T<\/jats:sup>\n (=DSM 106039\n T<\/jats:sup>\n =CECT 30156\n T<\/jats:sup>\n ).\n <\/jats:p>","DOI":"10.1099\/ijsem.0.004673","type":"journal-article","created":{"date-parts":[[2021,1,29]],"date-time":"2021-01-29T08:16:42Z","timestamp":1611908202000},"source":"Crossref","is-referenced-by-count":18,"title":["Sporofaciens musculi gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse"],"prefix":"10.1099","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9907-8953","authenticated-orcid":false,"given":"Torben S\u00f8lbeck","family":"Rasmussen","sequence":"first","affiliation":[{"name":"Section of Microbiology and Fermentation, Department of Food Science, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5503-9856","authenticated-orcid":false,"given":"Theresa","family":"Streidl","sequence":"additional","affiliation":[{"name":"Functional Microbiome Research Group, Institute of Medical Microbiology, RWTH University Hospital, Aachen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2244-7412","authenticated-orcid":false,"given":"Thomas C. 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