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Phylogenetic analysis based on the near full-length 16S rRNA sequences revealed that strain NLN63T<\/jats:sup> belongs to the genus \n \n Pelistega<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, having maximum similarity to \n \n Pelistega suis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> CCUG 64465T<\/jats:sup> (97.1\u200a%), \n \n Pelistega europaea<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> CCUG 39967T<\/jats:sup> (96.2\u200a%) and \n \n Pelistega indica<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> DSM 27484T<\/jats:sup> (96.2\u200a%), respectively. The phylogenomic tree built on 553 core genes from genomes of 20 species in the genus \n \n Pelistega<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and other adjacent genera further confirmed that strains NLN63T<\/jats:sup> and NLN82 form a distinct subline and exhibit specific phylogenetic affinity with \n \n P. europaea<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> CCUG 39967T<\/jats:sup>. In digital DNA\u2013DNA hybridization analyses, strain NLN63T<\/jats:sup> showed low estimated DNA reassociation values (21.4\u201322.6\u200a%) with the type strains of the species in the genus \n \n Pelistega<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. The DNA G+C contents of strains NLN63T<\/jats:sup> and NLN82 were 37.3 and 37.1\u2009mol%, respectively. Strain NLN63T<\/jats:sup> had a unique MALDI-TOF MS profile, contained Q-8 as the major quinone and C16\u200a:\u200a0<\/jats:sub>, summed feature 8 (C18\u200a:\u200a1<\/jats:sub>\n \u2009\u03c9<\/jats:italic>7c<\/jats:italic>\/C18\u200a:\u200a1<\/jats:sub>\n \u2009\u03c9<\/jats:italic>6c<\/jats:italic> or both) and summed feature 3 (C16\u200a:\u200a1<\/jats:sub>\n \u2009\u03c9<\/jats:italic>7c<\/jats:italic>\/C16\u200a:\u200a1<\/jats:sub>\n \u2009\u03c9<\/jats:italic>6c<\/jats:italic> or both) as the dominant fatty acids. Based upon these polyphasic characterization data obtained from the present study, a novel species of the genus \n \n Pelistega<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, Pelistega ratti<\/jats:italic> sp. nov., is proposed with NLN63T<\/jats:sup> (=GDMCC 1.1697T<\/jats:sup>=JCM 33788T<\/jats:sup>) as the type strain.<\/jats:p>","DOI":"10.1099\/ijsem.0.004733","type":"journal-article","created":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T16:16:24Z","timestamp":1615392984000},"source":"Crossref","is-referenced-by-count":6,"title":["Pelistega ratti sp. nov. from Rattus norvegicus of Hainan island"],"prefix":"10.1099","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6016-8188","authenticated-orcid":false,"given":"Xiaojun","family":"Zhou","sequence":"first","affiliation":[{"name":"Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, Hainan, PR China"},{"name":"Key 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