{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T20:16:04Z","timestamp":1789416964991,"version":"build-2803163510"},"reference-count":63,"publisher":"Microbiology Society","issue":"7","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["1R01AI136035"],"award-info":[{"award-number":["1R01AI136035"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,2]]},"abstract":"\n A previously unrecognized\n \n \n Rickettsia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n species was isolated in 1976 from a pool of\n Ixodes pacificus<\/jats:italic>\n ticks collected in 1967 from Tillamook County, Oregon, USA. The isolate produced low fever and mild scrotal oedema following intraperitoneal injection into male guinea pigs (\n Cavia porcellus<\/jats:italic>\n ). Subsequent serotyping characterized this isolate as distinct from recognized typhus and spotted fever group\n \n \n Rickettsia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n species; nonetheless, the isolate remained unevaluated by molecular techniques and was not identified to species level for the subsequent 30 years.\n Ixodes pacificus<\/jats:italic>\n is the most frequently identified human-biting tick in the western United States, and as such, formal identification and characterization of this potentially pathogenic\n \n \n Rickettsia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n species is warranted. Whole-genome sequencing of the Tillamook isolate revealed a genome 1.43 Mbp in size with 32.4 mol% G+C content. Maximum-likelihood phylogeny of core proteins places it in the transitional group of\n \n \n Rickettsia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n basal to both\n \n \n Rickettsia felis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n and\n \n \n Rickettsia asembonensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n . It is distinct from existing named species, with maximum average nucleotide identity of 95.1% to\n \n \n R. asembonensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n and maximum digital DNA\u2013DNA hybridization score similarity to\n \n \n R. felis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n at 80.1%. The closest similarity at the 16S rRNA gene (97.9%) and\n sca<\/jats:italic>\n 4 (97.5%\/97.6% respectively) is to\n Candidatus<\/jats:italic>\n \u2018Rickettsia senegalensis\u2019 and\n \n \n Rickettsia<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>\n sp. cf9, both isolated from cat fleas (\n Ctenocephalides felis<\/jats:italic>\n ). We characterized growth at various temperatures and in multiple cell lines. The Tillamook isolate grows aerobically in Vero E6, RF\/6A and DH82 cells, and growth is rapid at 28 \u00b0C and 32 \u00b0C. Using accepted genomic criteria, we propose the name\n Rickettsia tillamookensis<\/jats:italic>\n sp. nov., with the type strain Tillamook 23. Strain Tillamook 23 is available from the Centers for Disease Control and Prevention Rickettsial Isolate Reference Collection (WDCM 1093), Atlanta, GA, USA (CRIRC accession number RTI001\n T<\/jats:sup>\n ) and the Collection de Souches de l\u2019Unit\u00e9 des Rickettsies (WDCM 875), Marseille, France (CSUR accession number R5043). Using accepted genomic criteria, we propose the name\n Rickettsia tillamookensis<\/jats:italic>\n sp. nov., with the type strain Tillamook 23 (=CRIRC RTI001=R5043).\n <\/jats:p>","DOI":"10.1099\/ijsem.0.004880","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T13:19:10Z","timestamp":1625231950000},"source":"Crossref","is-referenced-by-count":24,"title":["Characterization of a novel transitional group Rickettsia species (Rickettsia tillamookensis sp. nov.) from the western black-legged tick, Ixodes pacificus"],"prefix":"10.1099","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2301-7150","authenticated-orcid":false,"given":"David T.","family":"Gauthier","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Old Dominion University, Norfolk, Virginia, 23529, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7175-3622","authenticated-orcid":false,"given":"Sandor E.","family":"Karpathy","sequence":"additional","affiliation":[{"name":"Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, 30329, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie L.","family":"Grizzard","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Old Dominion University, Norfolk, Virginia, 23529, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhwani","family":"Batra","sequence":"additional","affiliation":[{"name":"Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, Georgia, 30329, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori A.","family":"Rowe","sequence":"additional","affiliation":[{"name":"Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, Georgia, 30329, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher 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