{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:58:08Z","timestamp":1791421088187,"version":"4.3.3"},"reference-count":30,"publisher":"Microbiology Society","issue":"7","funder":[{"name":"MERLIN program Abyss"},{"name":"LabexMER Axis 3","award":["ANR-10-LABX-1"],"award-info":[{"award-number":["ANR-10-LABX-1"]}]},{"name":"Sino-French IRP 1211 MicrobSea"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,16]]},"abstract":"<jats:p>A novel extreme thermophilic and piezophilic chemoorganoheterotrophic archaeon, strain EXT12c<jats:sup>T<\/jats:sup>, was isolated from a hydrothermal chimney sample collected at a depth of 2496 m at the East Pacific Rise 9\u00b0 N. Cells were strictly anaerobic, motile cocci. The strain grew at NaCl concentrations ranging from 1 to 5 % (w\/v; optimum, 2.0%), from pH 6.0 to 7.5 (optimum, pH 6.5\u20137.0), at temperatures between 60 and 95 \u00b0C (optimum, 80\u201385 \u00b0C), and at pressures from 0.1 to at least 50 MPa (optimum, 30 MPa). Strain EXT12c<jats:sup>T<\/jats:sup> grew chemoorganoheterotrophically on complex proteinaceous substrates. Its growth was highly stimulated by the presence of elemental sulphur or <jats:sc>l<\/jats:sc>-cystine, which were reduced to hydrogen sulfide. The DNA G+C content was 54.58 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and concatenated ribosomal protein sequences showed that strain EXT12c<jats:sup>T<\/jats:sup> falls into the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.374\" xlink:type=\"simple\">Thermococcus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> and is most closely related to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.25374\" xlink:type=\"simple\">Thermococcus nautili<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> strain 30-1<jats:sup>T<\/jats:sup>. Overall genome relatedness index analyses (average nucleotide identity scores and <jats:italic>in silico<\/jats:italic> DNA\u2013DNA hybridizations) showed a sufficient genomic distance between the new genome and the ones of the <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.374\" xlink:type=\"simple\">Thermococcus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> type strains for the delineation of a new species. On the basis of genotypic and phenotypic data, strain EXT12c<jats:sup>T<\/jats:sup> is considered to represent a novel species, for which the name <jats:italic>Thermococcus henrietii<\/jats:italic> sp. nov. is proposed, with the type strain EXT12c<jats:sup>T<\/jats:sup> (=UBOCC M-2417<jats:sup>T<\/jats:sup>=DSM 111004<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.004895","type":"journal-article","created":{"date-parts":[[2021,7,16]],"date-time":"2021-07-16T17:16:08Z","timestamp":1626455768000},"source":"Crossref","is-referenced-by-count":11,"title":["Thermococcus henrietii sp. nov., a novel extreme thermophilic and piezophilic sulfur-reducing archaeon isolated from a deep-sea hydrothermal chimney"],"prefix":"10.1099","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8812-9081","authenticated-orcid":false,"given":"Karine","family":"Alain","sequence":"first","affiliation":[{"name":"Univ Brest, CNRS, Ifremer, Laboratoire de Microbiologie des Environnements Extr\u00eames LM2E, IUEM, Rue Dumont d\u2019Urville, F-29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwann","family":"Vince","sequence":"additional","affiliation":[{"name":"IRP 1211 MicrobSea, Sino-French Laboratory of Deep-Sea Microbiology, LM2E (Plouzan\u00e9, France)-KLAMBR, Xiamen, PR China"},{"name":"Univ Brest, CNRS, Ifremer, Laboratoire de Microbiologie des Environnements Extr\u00eames LM2E, IUEM, Rue Dumont d\u2019Urville, F-29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9162-0111","authenticated-orcid":false,"given":"Damien","family":"Courtine","sequence":"additional","affiliation":[{"name":"Univ Brest, CNRS, Ifremer, Laboratoire de Microbiologie des Environnements Extr\u00eames LM2E, IUEM, Rue Dumont d\u2019Urville, F-29280 Plouzan\u00e9, France"},{"name":"IRP 1211 MicrobSea, Sino-French Laboratory of Deep-Sea Microbiology, LM2E (Plouzan\u00e9, France)-KLAMBR, Xiamen, PR China"},{"name":"Aix Marseille Univ, CNRS, INSERM, CIML, Turing Centre for Living Systems, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5571-5228","authenticated-orcid":false,"given":"Lois","family":"Maignien","sequence":"additional","affiliation":[{"name":"IRP 1211 MicrobSea, Sino-French Laboratory of Deep-Sea Microbiology, LM2E (Plouzan\u00e9, France)-KLAMBR, Xiamen, PR China"},{"name":"Univ Brest, CNRS, Ifremer, Laboratoire de Microbiologie des Environnements Extr\u00eames LM2E, IUEM, Rue Dumont d\u2019Urville, F-29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Zeng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, PR China"},{"name":"IRP 1211 MicrobSea, Sino-French Laboratory of Deep-Sea Microbiology, LM2E (Plouzan\u00e9, France)-KLAMBR, Xiamen, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4784-090X","authenticated-orcid":false,"given":"Zongze","family":"Shao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, PR China"},{"name":"IRP 1211 MicrobSea, Sino-French Laboratory of Deep-Sea Microbiology, LM2E (Plouzan\u00e9, France)-KLAMBR, Xiamen, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3879-4400","authenticated-orcid":false,"given":"Mohamed","family":"Jebbar","sequence":"additional","affiliation":[{"name":"IRP 1211 MicrobSea, Sino-French Laboratory of Deep-Sea Microbiology, LM2E (Plouzan\u00e9, France)-KLAMBR, Xiamen, PR China"},{"name":"Univ Brest, CNRS, Ifremer, Laboratoire de Microbiologie des Environnements Extr\u00eames LM2E, IUEM, Rue Dumont d\u2019Urville, F-29280 Plouzan\u00e9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","volume-title":"The Microbiology of Deep-Sea. 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