{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T06:51:13Z","timestamp":1757314273459},"reference-count":40,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,30]]},"abstract":"Six novel facultatively anaerobic, Gram-stain-positive, rod-shaped, non-haemolytic bacteria (zg-320T<\/jats:sup>\/zg-336, zg-917T<\/jats:sup>\/zg-910 and zg-913T<\/jats:sup>\/zg-915) isolated from animal tissues and human faeces were found to belong to the genus \n \n Corynebacterium<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> based on the phylogenetic analyses of 16S rRNA gene and 262 core genes set. Based on the greatest degree of 16S rRNA similarity, zg-320T<\/jats:sup>\/zg-336 had the highest 16S rRNA gene similarity to \n \n Corynebacterium falsenii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> DSM 44353T<\/jats:sup> (97.51\u200a%), zg-917T<\/jats:sup>\/zg-910 to \n \n Corynebacterium coyleae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> DSM 44184T<\/jats:sup> (98.68\u200a%), and zg-913T<\/jats:sup>\/zg-915 to \n \n Corynebacterium afermentans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> subsp. \n \n lipophilum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> CIP 103500T<\/jats:sup> (98.79\u200a%). The three novel type strains had a relatively high DNA G+C content (61.2\u201364.4\u200amol%), low DNA relatedness and ANI values with their respective neighbours: 23.5\/72.7\u200a%, 25.0\/72.3%and 22.6\/73.1\u200a% (zg-320T<\/jats:sup> vs. \n \n Corynebacterium auriscanis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> CIP 106629T<\/jats:sup>, \n \n Corynebacterium resistens<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> DSM 45100T<\/jats:sup> and \n \n Corynebacterium suicordis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> DSM 45110T<\/jats:sup>); 24.4\/82.3% and 23.7\/81.3\u200a% (zg-917T<\/jats:sup> vs. \n \n C. coyleae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> DSM 44184T<\/jats:sup> and \n \n Corynebacterium jeddahense<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> JCBT<\/jats:sup>); 26.8\/83.7% and 27.7\/84.4\u200a% (zg-913T<\/jats:sup> vs. \n \n Corynebacterium mucifaciens<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> ATCC 700355T<\/jats:sup> and \n \n C. afermentans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> subsp. \n \n lipophilum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> CCUG 32105T<\/jats:sup>). The three novel species had C16\u200a:\u200a0<\/jats:sub>, C18\u200a:\u200a0<\/jats:sub>, C18\u200a:\u200a1<\/jats:sub>\n \u2009\u03c9<\/jats:italic>9c<\/jats:italic> and C18\u200a:\u200a0<\/jats:sub> ante\/C18\u200a:\u200a2<\/jats:sub>\n \u2009\u03c9<\/jats:italic>6,9c<\/jats:italic> as the major cellular fatty acids; MK-8(H2<\/jats:sub>) in strain zg-917T<\/jats:sup> and MK-9(H2<\/jats:sub>) in strains zg-320T<\/jats:sup> and zg-913T<\/jats:sup> were found to be the major respiratory quinones. For the three novel species, the detected major polar lipids included diphosphatidylglycerol, phosphatidyl inositol mannoside, phosphatidylglycerol and phosphatidylinositol, the cell-wall peptidoglycan was based on meso<\/jats:italic>-DAP, and the whole-cell sugars mainly included ribose, arabinose and galactose. The three novel species grew optimally at 35\u201337\u2009\u00b0C, 0.5\u200a% (w\/v) NaCl and pH 7.0\u20138.0; notably, they were tolerant of 10.5\u200a% (w\/v) NaCl. Based on the results of these comprehensive analyses, three novel species in the genus \n \n Corynebacterium<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> are proposed, aptly named Corynebacterium zhongnanshanii<\/jats:italic> sp. nov. (zg-320T<\/jats:sup> = GDMCC 1.1719T<\/jats:sup> = JCM 34106T<\/jats:sup>), Corynebacterium lujinxingii<\/jats:italic> sp. nov. (zg-917T<\/jats:sup> = GDMCC 1.1707T<\/jats:sup> = JCM 34094T<\/jats:sup>) and Corynebacterium wankanglinii<\/jats:italic> sp. nov. (zg-913T<\/jats:sup> = GDMCC 1.1706T<\/jats:sup> = JCM 34398T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.005069","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T16:17:16Z","timestamp":1638289036000},"source":"Crossref","is-referenced-by-count":22,"title":["Corynebacterium zhongnanshanii sp. nov. isolated from trachea of Marmota himalayana, Corynebacterium lujinxingii sp. nov. and Corynebacterium wankanglinii sp. nov. from human faeces"],"prefix":"10.1099","volume":"71","author":[{"given":"Gui","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Research Units of Discovery of Unknown Bacteria 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