{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:50:09Z","timestamp":1790189409002,"version":"4.1.0"},"reference-count":33,"publisher":"Microbiology Society","issue":"1","license":[{"start":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T00:00:00Z","timestamp":1643760000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007917","name":"Agricultural Research Service","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007917","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,2]]},"abstract":"<jats:p>Three novel, anaerobic, Gram-positive bacteria were isolated from the eggshell of two separate white leghorn chicken flocks and the ileum of a healthy pig, and designated MMM721<jats:sup>T<\/jats:sup>, ISU324 and PIG517 respectively. Cells were pleomorphic and capable of forming long chains of rods or coccoid clusters. Phylogenetic analysis of the 16S rRNA gene sequences identified these strains to be within the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5316\" xlink:type=\"simple\">Turicibacter<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, of which only one species, <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5317\" xlink:type=\"simple\">Turicibacter sanguinis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, has been formally described. However, whole genome sequencing of novel isolates returned a digital DNA\u2013DNA hybridization value of 22.5\u200a% and average nucleotide identity (ANI) values of 76.4\u200a% (ANIb) and 86.0\u200a% (ANIm), indicating divergence between the type strain MMM721<jats:sup>T<\/jats:sup> and <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5317\" xlink:type=\"simple\">T. sanguinis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, suggesting the strains represented a novel species. The major fatty acid methyl esters of strain MMM721<jats:sup>T<\/jats:sup> were C<jats:sub>16\u200a:\u200a0<\/jats:sub>, C<jats:sub>18\u200a:\u200a1<\/jats:sub>\u2009\u03c97<jats:italic>c<\/jats:italic> and C<jats:sub>18\u200a:\u200a0<\/jats:sub>. The strains mainly produced the volatile fatty acid lactate, along with smaller amounts of acetate and butyrate. Together, these data indicate that MMM721<jats:sup>T<\/jats:sup>, along with ISU324 and PIG517, represent a novel species within the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5316\" xlink:type=\"simple\">Turicibacter<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. We propose the name <jats:italic>Turicibacter bilis<\/jats:italic> sp. nov. for the species. The type strain is MMM721<jats:sup>T<\/jats:sup> (=ATCC TSD-238<jats:sup>T<\/jats:sup>=CCUG 74757<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.005153","type":"journal-article","created":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T17:18:36Z","timestamp":1643303916000},"source":"Crossref","is-referenced-by-count":48,"title":["Turicibacter bilis sp. nov., a novel bacterium isolated from the chicken eggshell and swine ileum"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1197-0179","authenticated-orcid":false,"given":"Joel J.","family":"Maki","sequence":"first","affiliation":[{"name":"Interdepartmental Microbiology Graduate Program, Iowa State University, Ames, IA, 50011, USA"},{"name":"Oak Ridge Institute for Science and Education, ARS Research Participation Program, Oak Ridge, Tennessee 37830, USA"},{"name":"Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center, Agricultural Research Service, United 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