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They were characterized by a polyphasic approach including 16S rRNA gene sequence analysis, core-genome analysis, average nucleotide identity (ANI) analysis, digital DNA\u2013DNA hybridization (dDDH), determination of G+C content, screening of antibiotic resistance genes, and various phenotypic analyses. Initial analysis of 16S rRNA gene sequence identities indicated that strain GAL7T<\/jats:sup> was potentially very closely related to \n \n Enterococcus casseliflavus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n Enterococcus gallinarum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, having 99.5\u201399.9\u200a%\u2009sequence similarity. However, further analysis of whole genome sequences revealed a genome size of 3.69\u2009Mb, DNA G+C content of 42.35\u2009mol%, and low dDDH and ANI values between the genomes of strain GAL7T<\/jats:sup> and closest phylogenetic relative \n \n E. casseliflavus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> NBRC 100478T<\/jats:sup> of 59.0 and 94.5\u200a%, respectively, indicating identification of a putative new \n \n Enterococcus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species. In addition, all novel strains encoded the atypical vancomycin-resistance gene vanC-4<\/jats:italic>. Results of phylogenomic, physiological and phenotypic characterization confirmed that strain GAL7T<\/jats:sup> represented a novel species within the genus \n \n Enterococcus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, for which the name Enterococcus innesii<\/jats:italic> sp. nov. is proposed. The type strain is GAL7T<\/jats:sup> (=DSM 112306T<\/jats:sup>=NCTC 14608T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.005168","type":"journal-article","created":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T17:16:45Z","timestamp":1639761405000},"source":"Crossref","is-referenced-by-count":23,"title":["Enterococcus innesii sp. nov., isolated from the wax moth Galleria mellonella"],"prefix":"10.1099","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3558-1143","authenticated-orcid":false,"given":"Harriet C. C.","family":"Gooch","sequence":"first","affiliation":[{"name":"Dept. 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