{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T03:08:16Z","timestamp":1780369696149,"version":"3.54.1"},"reference-count":35,"publisher":"Microbiology Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,14]]},"abstract":"Gram-positive coccoid bacteria were isolated from the nasal cavities of pigs and calves as well as from axillar and inguinal skin regions of pigs. Phylogenetic analysis of seven strains based on complete genome, 16S rRNA, hsp60<\/jats:italic>, dnaJ<\/jats:italic>, rpoB<\/jats:italic> and sodA<\/jats:italic> gene sequences and MALDI-TOF MS profiles revealed that they belonged to the genus \n \n Macrococcus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> with the closest relatedness to \n \n Macrococcus canis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, \n \n Macrococcus caseolyticus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> subsp. \n \n caseolyticus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n Macrococcus caseolyticus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> subsp. \n \n hominis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. DNA relatedness of the type strain JEK37T<\/jats:sup> with the type strains of \n \n M. canis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, \n \n M. caseolyticus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> subsp. \n \n caseolyticus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n M. caseolyticus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> subsp. \n \n hominis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> was 23.4, 23.1 and 23.0\u200a% by digital DNA\u2013DNA hybridization and 80.39, 80.45 and 80.87\u200a% by average nucleotide identity (ANI) calculations, confirming that they do not belong to the same species. The DNA G+C content of JEK37T<\/jats:sup> was 35.65 mol%. The novel strains can be differentiated from \n \n M. canis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> KM 45013T<\/jats:sup> by the ability to fermentate d<\/jats:sc>-ribose and by the absence of DNAase production and haemolysis, from \n \n M. caseolyticus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> subsp. \n \n caseolyticus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> CCUG 15606T<\/jats:sup> by the ability to fermentate sucrose and from both species by the inability to grow in 9 and 12% NaCl. They differ from M. caseolyiticus<\/jats:italic> subsp. hominis<\/jats:italic> by the presence of \u03b1-glucosidase. The most common fatty acids of JEK37T<\/jats:sup> were C14\u200a:\u200a0<\/jats:sub>, C18\u200a:\u200a1<\/jats:sub>\u2009\u03c99c<\/jats:italic> and C18\u200a:\u200a0<\/jats:sub>. Known polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, aminolipid, aminoglycolipid, aminophospholipid, glycolipid and phospholipid. Cell-wall peptidoglycan of JEK37T<\/jats:sup> was of type A3\u03b1 l<\/jats:sc>-Lys\u2013Gly2<\/jats:sub>\u2013L-Ser\u2013Gly (similar to A11.3) and the respiratory quinolone was menaquinone 6. Based on their genotypic and chemotaxonomic characteristics, these strains represent a novel species of the genus \n \n Macrococcus<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, for which we propose the name Macrococcus armenti<\/jats:italic> sp. nov. 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