{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T07:10:15Z","timestamp":1790493015897,"version":"4.1.0"},"reference-count":15,"publisher":"Microbiology Society","issue":"5","funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP21fk0108129h0702"],"award-info":[{"award-number":["JP21fk0108129h0702"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP21lm0203007"],"award-info":[{"award-number":["JP21lm0203007"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002066","name":"GlaxoSmithKline foundation","doi-asserted-by":"crossref","award":["A-32"],"award-info":[{"award-number":["A-32"]}],"id":[{"id":"10.13039\/501100002066","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP21K16118"],"award-info":[{"award-number":["JP21K16118"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP21K08196"],"award-info":[{"award-number":["JP21K08196"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Japan Intractable Diseases (Nanbyo) Research Foundation","award":["2020B02"],"award-info":[{"award-number":["2020B02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,23]]},"abstract":"A slowly growing mycobacteria, identified as strain TY59T<\/jats:sup>, was isolated from sputum of an elderly man with pneumonia. Sequencing of the 16S rRNA gene indicated that this strain was similar to members of the \n \n Mycobacterium avium<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> complex and closely related species. Strain TY59T<\/jats:sup> has highest 16S rRNA gene sequence similarities to the type strains of \n \n Mycobacterium colombiense<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (99.80\u200a% sequence similarity), \n \n Mycobacterium vulneris<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (99.74\u200a%), \n \n Mycobacterium timonense<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (99.54\u200a%), \n \n Mycobacterium avium<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> subsp. \n \n avium<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (99.54\u200a%) and \n \n Mycobacterium avium<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> subsp. \n \n silvaticum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (99.54\u200a%). Analysis of the internal transcribed spacer (ITS) and DNA-directed RNA polymerase subunit beta (rpoB<\/jats:italic>) sequences gave similar results to the 16S rRNA gene analysis. The closest species to strain TY59T<\/jats:sup> were \n \n M. colombiense<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n M. vulneris<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> with 97.90\u201398.25\u200a%\u2009identity in ITS and 96.4\u201396.6\u200a% in rpoB<\/jats:italic>. The strain's 65\u2009kDa heat shock protein (hsp65<\/jats:italic>) gene was different from those of \n \n M. vulneris<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, \n \n M. colombiense<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n M. avium<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> subsp. \n \n silvaticum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> with 72.4\u201374.2\u200a% identity. Average nucleotide identity results showed a 93.4\u200a% match to \n \n M. vulneris<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> as the maximum value. Phenotypically, the non-chromogenicity, rough colonies, growth at 42 \u00b0C, negative results for nitrate reduction, \u03b2-glucosidase and Tween 80 hydrolysis, and positive results for catalase activity set this strain apart from closely related species. We propose that Mycobacterium senriense<\/jats:italic> sp. nov. is a novel species of slowly growing mycobacteria. The type strain is TY59T<\/jats:sup> (RIMD 1371001T<\/jats:sup>=CIP 111917T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.005378","type":"journal-article","created":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T17:18:01Z","timestamp":1653326281000},"source":"Crossref","is-referenced-by-count":11,"title":["Mycobacterium senriense sp. nov., a slowly growing, non-scotochromogenic species, isolated from sputum of an elderly man"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3438-4201","authenticated-orcid":false,"given":"Yuko","family":"Abe","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9767-7899","authenticated-orcid":false,"given":"Kiyoharu","family":"Fukushima","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, National Hospital Organization Osaka Toneyama Medical Center, 5-1-1 Toneyama Toyonaka, Osaka, Japan"},{"name":"Laboratory of Host Defense, World Premier Institute Immunology Frontier Research Center (WPI-IFReC), Osaka University, 3-1 Yamadaoka, Suita, Osaka, Japan"},{"name":"Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Infection Metagenomics, Genome Information Research Center, Research Institute for Microbial Diseases (RIMD), Osaka University, 3-1 Yamadaoka, Suita, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4280-3074","authenticated-orcid":false,"given":"Takayuki","family":"Niitsu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Nabeshima","sequence":"additional","affiliation":[{"name":"Fujii Memorial Research Institute, Otsuka Pharmaceutical Company, Ltd., 520-0106 Shiga, Japan"},{"name":"Laboratory of Host Defense, World Premier Institute Immunology Frontier Research Center (WPI-IFReC), Osaka University, 3-1 Yamadaoka, Suita, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuharu","family":"Nagahama","sequence":"additional","affiliation":[{"name":"Fujii Memorial Research Institute, Otsuka Pharmaceutical Company, Ltd., 520-0106 Shiga, 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