{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T18:50:08Z","timestamp":1792090208648,"version":"4.4.0"},"reference-count":32,"publisher":"Microbiology Society","issue":"4","funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP21ae0121035"],"award-info":[{"award-number":["JP21ae0121035"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP21J11100"],"award-info":[{"award-number":["JP21J11100"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["19H05679"],"award-info":[{"award-number":["19H05679"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["20K05792"],"award-info":[{"award-number":["20K05792"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,13]]},"abstract":"\n \n \n Faecalibacterium prausnitzii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> is one of the most important butyrate-producing bacteria in the human gut. Previous studies have suggested the presence of several phylogenetic groups, with differences at the species level, in the species, and a taxonomic re-evaluation is thus essential for further understanding of ecology of the important human symbiont. Here we examine the phenotypic, physiological, chemotaxonomic and phylogenomic characteristics of six \n \n F. prausnitzii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> strains (BCRC 81047T<\/jats:sup>=ATCC 27768T<\/jats:sup>, A2-165T<\/jats:sup>=JCM 31915T<\/jats:sup>, APC918\/95b=JCM 39207, APC942\/30\u22122=JCM 39208, APC924\/119=JCM 39209 and APC922\/41\u22121T<\/jats:sup>=JCM 39210T<\/jats:sup>) deposited in public culture collections with two reference strains of \n \n Faecalibacterium butyricigenerans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> JCM 39212T<\/jats:sup> and \n \n Faecalibacterium longum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> JCM 39211T<\/jats:sup>. \n \n Faecalibacterium<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> sp. JCM 17207T<\/jats:sup> isolated from caecum of broiler chicken was also included. Three strains of \n \n F. prausnitzii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (BCRC 81047T<\/jats:sup>, JCM 39207 and JCM 39209) shared more than 96.6\u200a% average nucleotide identity (ANI) and 69.6\u200a% digital DNA\u2013DNA hybridization (dDDH) values, indicating that the three strains are members of the same species. On the other hand, the remaining three strains of \n \n F. prausnitzii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> (JCM 31915T<\/jats:sup>, JCM 39208 and JCM 39210T<\/jats:sup>) were clearly separated from the above three strains based on the ANI and dDDH values. Rather, JCM 39208 showed ANI and dDDH values over the cut-off values of species discrimination (>70\u200a%\u2009dDDH and >95\u201396\u200a%\u2009ANI) with \n \n F. longum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> JCM 39211T<\/jats:sup>, whereas JCM 31915T<\/jats:sup>, JCM 39210T<\/jats:sup> and JCM 17207T<\/jats:sup> did not share dDDH and ANI values over the currently accepted cut-off values with any of the tested strains, including among them. Furthermore, the cellular fatty acid patterns of these strains were slightly different from other \n \n F. prausnitzii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> strains. Based on the collected data, \n \n F. prausnitzii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> JCM 31915T<\/jats:sup>, \n \n F. prausnitzii<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> JCM 39210T<\/jats:sup> and \n \n Faecalibacterium<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> sp. JCM 17207T<\/jats:sup> represent three novel species of the genus \n \n Faecalibacterium<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, for which the names Faecalibacterium duncaniae<\/jats:italic> sp. nov. (type strain JCM 31915T<\/jats:sup>=DSM 17677T<\/jats:sup>=A2-165T<\/jats:sup>), Faecalibacterium hattorii<\/jats:italic> sp. nov. (type strain JCM 39210T<\/jats:sup>=DSM 107841T<\/jats:sup>=APC922\/41-1T<\/jats:sup>) and Faecalibacterium gallinarum<\/jats:italic> sp. nov. (type strain JCM 17207T<\/jats:sup>=DSM 23680T<\/jats:sup>=ic1379T<\/jats:sup>) are proposed.<\/jats:p>","DOI":"10.1099\/ijsem.0.005379","type":"journal-article","created":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T15:16:27Z","timestamp":1649862987000},"source":"Crossref","is-referenced-by-count":89,"title":["Genome-based, phenotypic and chemotaxonomic classification of Faecalibacterium strains: proposal of three novel species Faecalibacterium duncaniae sp. nov., Faecalibacterium hattorii sp. nov. and Faecalibacterium gallinarum sp. nov."],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3622-4642","authenticated-orcid":false,"given":"Mitsuo","family":"Sakamoto","sequence":"first","affiliation":[{"name":"Microbe Division\/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi","family":"Sakurai","sequence":"additional","affiliation":[{"name":"Microbe Division\/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Tanno","sequence":"additional","affiliation":[{"name":"Department of Food, Aroma and Cosmetic Chemistry, Faculty of Bioindustry, Tokyo University of Agriculture, Hokkaido 099-2493, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takao","family":"Iino","sequence":"additional","affiliation":[{"name":"Microbe Division\/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moriya","family":"Ohkuma","sequence":"additional","affiliation":[{"name":"Microbe Division\/Japan Collection of Microorganisms, RIKEN 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