{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T10:37:59Z","timestamp":1770287879425,"version":"3.49.0"},"reference-count":28,"publisher":"Microbiology Society","issue":"9","license":[{"start":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T00:00:00Z","timestamp":1663113600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,14]]},"abstract":"\n \n \n Legionella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>-like isolates, strains 27fs60, 30fs61 and 30cs62T<\/jats:sup>, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy. Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32\u201337\u2009\u00b0C (with an optimum at 32\u2009\u00b0C) on buffered charcoal\u2013yeast extract agar with l<\/jats:sc>-cysteine, glycine\u2013vancomycin\u2013polymyxin B\u2013cycloheximide agar and Wadowsky\u2013Yee medium agar. The strains showed positive reactions for oxidase, hippurate and gelatinase and a weakly positive reaction for catalase. Based on the EUCAST cut-off, strain 30cs62T<\/jats:sup> was resistant to ciprofloxacin (5\u2009mg\u2009l\u22121<\/jats:sup>). The mip<\/jats:italic> and rpoB<\/jats:italic> gene sequences of the three strains showed close matches to those of \n \n Legionella quateirensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> ATCC 49507T<\/jats:sup> with similarity values of 98.2 and 94.5\u200a%, respectively. Whole genome sequencing of the three strains was performed, resulting in G+C contents of 39.0, 39.1 and 39.0\u2009mol%, respectively. The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32\u200a% \n \n L<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. \n \n quateirensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> NCTC 12376T<\/jats:sup>, 91.45\u200a% \n \n L<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. \n \n quateirensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> ATCC 49507T<\/jats:sup> and 91.45\u200a% \n \n L<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>. \n \n quateirensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> ATCC 49507T<\/jats:sup>, respectively. The digital DNA\u2013DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic \n \n Legionella<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species (\n \n L. quateirensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> ATCC 49507T<\/jats:sup>, \u226440.10\u200a% DNA\u2013DNA relatedness). The concatenated phylogenetic tree based on 16S rRNA<\/jats:italic>, mip, rpoB<\/jats:italic> and rnpB<\/jats:italic> genes, shows a close relationship with \n \n L. quateirensis<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> ATCC 49507T<\/jats:sup>. The results obtained confirm the status of an independent species. The name proposed for this species is Legionella bononiensis<\/jats:italic> sp. nov. with 30cs62T<\/jats:sup> (=ATCC TSD-262T<\/jats:sup>=DSM 112526T<\/jats:sup>) as the type strain.<\/jats:p>","DOI":"10.1099\/ijsem.0.005512","type":"journal-article","created":{"date-parts":[[2022,9,29]],"date-time":"2022-09-29T16:16:35Z","timestamp":1664468195000},"source":"Crossref","is-referenced-by-count":4,"title":["Legionella bononiensis sp. nov., isolated from a hotel water distribution system in northern Italy"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5916-9634","authenticated-orcid":false,"given":"Luna","family":"Girolamini","sequence":"first","affiliation":[{"name":"European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group for Legionella Infections (ESGLI), Basel, Switzerland"},{"name":"Department of Biological, Geological and Environmental Sciences, University of Bologna, via San Giacomo 12, 40126 Bologna (BO), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6333-3492","authenticated-orcid":false,"given":"Maria Rosaria","family":"Pascale","sequence":"additional","affiliation":[{"name":"Department of Biological, Geological and Environmental Sciences, University of Bologna, via San Giacomo 12, 40126 Bologna (BO), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8389-7460","authenticated-orcid":false,"given":"Silvano","family":"Salaris","sequence":"additional","affiliation":[{"name":"Department of Biological, Geological and Environmental Sciences, University of Bologna, via San Giacomo 12, 40126 Bologna (BO), Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6966-3115","authenticated-orcid":false,"given":"Marta","family":"Mazzotta","sequence":"additional","affiliation":[{"name":"Department of Biological, Geological and Environmental 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