{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T17:13:59Z","timestamp":1781198039996,"version":"3.54.1"},"reference-count":41,"publisher":"Microbiology Society","issue":"10","funder":[{"DOI":"10.13039\/100012947","name":"Institut de Recherche pour le D\u00e9veloppement","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100012947","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,19]]},"abstract":"<jats:p>An anaerobic, hydrogenotrophic methane-producing archaeon was isolated from an alkaline thermal spring (42\u2009\u00b0C, pH 9.0) in New Caledonia. This methanogen, designated strain CAN<jats:sup>T<\/jats:sup>, is alkaliphilic, thermotolerant, with Gram-positive staining non-motile cells. Strain CAN<jats:sup>T<\/jats:sup> grows autotrophically using hydrogen exclusively as an energy source and carbon dioxide as the sole carbon source (without the requirement of yeast extract or other organic compounds). It grows at 20\u201345\u2009\u00b0C (optimum, 45\u2009\u00b0C) and pH 7.3\u20139.7 (optimum, pH 9.0). NaCl is not required for growth (optimum 0\u200a%) but is tolerated up to 1.5\u200a%. It resists novobiocin, streptomycin and vancomycin but is inhibited by ampicillin and penicillin, among other antibiotics. The genome consists of a circular chromosome (2.2\u2009Mb) containing 2126 predicted protein-encoding genes with a G+C content of 36.4\u2009mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain CAN<jats:sup>T<\/jats:sup> is a member of the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.94\" xlink:type=\"simple\">Methanobacterium<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, most closely related to the alkaliphilic <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.96\" xlink:type=\"simple\">Methanobacterium alcaliphilum<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> WeN4<jats:sup>T<\/jats:sup> with 98.5\u200a% 16S rRNA gene sequence identity. The genomes of strain CAN<jats:sup>T<\/jats:sup> and <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.96\" xlink:type=\"simple\">M. alcaliphilum<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> DSM 3459, sequenced in this study, share 71.6\u200a% average nucleotide identity and 14.0\u200a% digital DNA\u2013DNA hybridization. Therefore, phylogenetic and physiological results indicate that strain CAN<jats:sup>T<\/jats:sup> represents a novel species, for which the name <jats:italic>Methanobacterium alkalithermotolerans<\/jats:italic> sp. nov. is proposed, and strain CAN<jats:sup>T<\/jats:sup> (=DSM 102889<jats:sup>T<\/jats:sup>= JCM 31304<jats:sup>T<\/jats:sup>) is assigned as the type strain.<\/jats:p>","DOI":"10.1099\/ijsem.0.005554","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T16:17:24Z","timestamp":1666196244000},"source":"Crossref","is-referenced-by-count":16,"title":["Methanobacterium alkalithermotolerans sp. nov., a novel alkaliphilic and hydrogen-utilizing methanogen isolated from an alkaline geothermal spring (La Crouen, New Caledonia)"],"prefix":"10.1099","volume":"72","author":[{"given":"Nan","family":"Mei","sequence":"first","affiliation":[{"name":"Present address: School of Chemistry, Chemical Engineering, and Life Science, Wuhan University of Technology, Wuhan 430070, PR China"},{"name":"Aix Marseille Univ, Universit\u00e9 de Toulon, CNRS, IRD, MIO, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Postec","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, Universit\u00e9 de Toulon, CNRS, IRD, MIO, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manon","family":"Bartoli","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, Universit\u00e9 de Toulon, CNRS, IRD, MIO, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Vandecasteele","sequence":"additional","affiliation":[{"name":"INRAE, US 1426, GeT-PlaGe, Genotoul, Castanet-Tolosan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Wils","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, Universit\u00e9 de Toulon, CNRS, IRD, MIO, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Gil","sequence":"additional","affiliation":[{"name":"INRAE, US 1426, GeT-PlaGe, Genotoul, Castanet-Tolosan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Monnin","sequence":"additional","affiliation":[{"name":"GET UMR5563 (CNRS\/UPS\/IRD\/CNES), G\u00e9osciences Environnement Toulouse, 14 Avenue Edouard Belin, 31400 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Pelletier","sequence":"additional","affiliation":[{"name":"UMR G\u00e9oazur, Centre IRD de Noum\u00e9a, 101 Promenade Roger Laroque, BP A5 \u2013 98848 Noum\u00e9a cedex, New Caledonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gael","family":"Erauso","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, Universit\u00e9 de Toulon, CNRS, IRD, MIO, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4071-9848","authenticated-orcid":false,"given":"Marianne","family":"Qu\u00e9m\u00e9neur","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ, Universit\u00e9 de Toulon, CNRS, IRD, MIO, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2018.05.021"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1006\/anae.2000.0345"},{"key":"R3","first-page":"1","volume-title":"Methanobacterium","author":"Boone","year":"2015"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-021-00918-8"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-2-357"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02994-0"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.027540-0"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-36-3-380"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1144\/M51-2016-9"},{"key":"R10","volume-title":"Geochemistry of low temperature geothermal systems in New Caledonia. 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