{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:43:56Z","timestamp":1792691036335,"version":"4.5.0"},"reference-count":41,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T00:00:00Z","timestamp":1666310400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"crossref","award":["2017\/06731-8 and 2019\/20302-8"],"award-info":[{"award-number":["2017\/06731-8 and 2019\/20302-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"crossref","award":["6NU1ROT000006-01-04"],"award-info":[{"award-number":["6NU1ROT000006-01-04"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007917","name":"Agricultural Research Service, USDA.","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007917","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,21]]},"abstract":"<jats:p>Two spirochetes (designated strains LGVF01 and LGVF02<jats:sup>T<\/jats:sup>) were isolated from soil samples in San Juan, Puerto Rico in HAN media after selection using a combination of ELISA, agar plating, and colony screening by Fluorescent Antibody Testing (FAT) and PCR for <jats:italic>lipL32<\/jats:italic> and <jats:italic>secY<\/jats:italic>. Isolates were helix-shaped, aerobic, fast-growing, and highly motile. Genome sequence analysis indicated that both strains should be classified as members of a novel species within the pathogenic (P1) clade of the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7893\" xlink:type=\"simple\">Leptospira<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. The average nucleotide identity between the two strains was 99.2\u200a%, but below 93.2\u200a% when compared to any previously described leptospiral species. Serotyping of strain LGVF02<jats:sup>T<\/jats:sup> indicates that it does not belong within any serogroup of <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.7893\" xlink:type=\"simple\">Leptospira<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> suggesting it also represents a new serovar. Collectively, strains LGVF01 and LGVF02<jats:sup>T<\/jats:sup> represent a new species of pathogenic leptospires for which the name <jats:italic>Leptospira sanjuanensis<\/jats:italic> sp. nov. is proposed. The type strain is LGVF02<jats:sup>T<\/jats:sup> (=NVSL-LGVF02<jats:sup>T<\/jats:sup>=KIT0302<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.005560","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T17:16:55Z","timestamp":1666199815000},"source":"Crossref","is-referenced-by-count":51,"title":["Leptospira sanjuanensis sp. nov., a pathogenic species of the genus Leptospira isolated from soil in Puerto Rico"],"prefix":"10.1099","volume":"72","author":[{"given":"Luis G. V.","family":"Fernandes","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento de Vacinas, Instituto Butantan, S\u00e3o Paulo 05503-900, Brazil"},{"name":"Infectious Bacterial Diseases Research Unit, ARS, United States Department of Agriculture, Ames, IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan E.","family":"Stone","sequence":"additional","affiliation":[{"name":"The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandler C.","family":"Roe","sequence":"additional","affiliation":[{"name":"The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marga G. A.","family":"Goris","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infection Prevention, OIE and National Collaborating Centre for Reference and Research on Leptospirosis, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"van der Linden","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infection Prevention, OIE and National Collaborating Centre for Reference and Research on Leptospirosis, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason W.","family":"Sahl","sequence":"additional","affiliation":[{"name":"The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Wagner","sequence":"additional","affiliation":[{"name":"The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9478-1316","authenticated-orcid":false,"given":"Jarlath E.","family":"Nally","sequence":"additional","affiliation":[{"name":"Infectious Bacterial Diseases Research Unit, ARS, United States Department of Agriculture, Ames, IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.6084\/m9.figshare.20334444"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/s1473-3099(03)00830-2"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003898"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-45059-8_6"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.296-326.2001"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-3-851"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007270"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.003480"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004713"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.005148"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00816"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000144"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12551"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1590\/S0325-75412010000200012"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009959"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-66526-4"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2011.00347.x"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1186\/1476-0711-5-28"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.03.014"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1128\/jb.88.6.1618-1623.1964"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00173-15"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-81400-7"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112963"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw569"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty191"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-5-113"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx281"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.066597-0"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00670-06"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-0997-x"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0686-2"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.332"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl158"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3176"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-6-2"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1039\/C5AY02550H"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906412106"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004403"},{"key":"R41","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0580-9517(08)70493-8","article-title":"Serological typing methods of leptospires","volume":"11","author":"Dikken","year":"1978","journal-title":"Methods Microbiol"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.005560?crawler=true&mimetype=application\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T19:24:38Z","timestamp":1702581878000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.005560"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,21]]},"references-count":41,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,10,21]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.005560","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,21]]}}}