{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T10:24:23Z","timestamp":1783160663144,"version":"3.54.6"},"reference-count":66,"publisher":"Microbiology Society","issue":"10","funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["DFG Priority Program 1374"],"award-info":[{"award-number":["DFG Priority Program 1374"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,21]]},"abstract":"<jats:p>Three strains (H4-D09<jats:sup>T<\/jats:sup>, S2-D11 and S9-F39) of a member of the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\nParacoccus\n<\/jats:named-content>\n                  <\/jats:italic> attributed to a novel species were isolated from topsoil of temperate grasslands. The genome sequence of the type strain H4-D09<jats:sup>T<\/jats:sup> exhibited a complete set of genes required for denitrification as well as methylotrophy. The genome of H4-D09<jats:sup>T<\/jats:sup> included genes for two alternative pathways of formaldehyde oxidation. Besides the genes for the canonical glutathione (GSH)-dependent formaldehyde oxidation pathway, all genes for the tetrahydrofolate\u2013formaldehyde oxidation pathway were identified. The strain has the potential to utilize methanol and\/or methylamine as a single carbon source as evidenced by the presence of methanol dehydrogenase (<jats:italic>mxaFI<\/jats:italic>) and methylamine dehydrogenase (<jats:italic>mau<\/jats:italic>) genes. Apart from dissimilatory denitrification genes (<jats:italic>narA<\/jats:italic>, <jats:italic>nirS<\/jats:italic>, <jats:italic>norBC<\/jats:italic> and <jats:italic>nosZ<\/jats:italic>), genes for assimilatory nitrate (<jats:italic>nasA<\/jats:italic>) and nitrite reductases (<jats:italic>nirBD<\/jats:italic>) were also identified. The results of phylogenetic analysis based on 16S rRNA genes coupled with riboprinting revealed that all three strains represented the same species of genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\nParacoccus\n<\/jats:named-content>\n                  <\/jats:italic>. Core genome phylogeny of the type strain H4-D09<jats:sup>T<\/jats:sup> indicated that <jats:italic>\n                     <jats:named-content content-type=\"species\">\nParacoccus thiocyanatus\n<\/jats:named-content>\n                  <\/jats:italic> and <jats:italic>\n                     <jats:named-content content-type=\"species\">\nParacoccus denitrificans\n<\/jats:named-content>\n                  <\/jats:italic> are the closest phylogenetic neighbours. The average nucleotide index (ANI) and digital DNA\u2013DNA hybridization (dDDH) with the closest phylogenetic neighbours revealed genetic differences at the species level, which were further substantiated by differences in several physiological characteristics. The major respiratory quinone is Q-10, and the predominant cellular fatty acids are C<jats:sub>18\u200a:\u200a1<\/jats:sub>\u03c97<jats:italic>c<\/jats:italic>, C<jats:sub>19\u200a:\u200a0<\/jats:sub>cyclo \u03c97<jats:italic>c<\/jats:italic>, and C<jats:sub>16\u200a:\u200a0<\/jats:sub>, which correspond to those detected in other members of the genus. The polar lipid profile consists of a diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), aminolipid (AL), glycolipid (GL) and an unidentified lipid (L).<\/jats:p>\n               <jats:p>On the basis of our results, we concluded that the investigated isolates represent a novel species of the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\nParacoccus\n<\/jats:named-content>\n                  <\/jats:italic>, for which the name <jats:italic>Paracoccus methylovorus<\/jats:italic> sp. nov. (type strain H4-D09<jats:sup>T<\/jats:sup>=LMG 31941<jats:sup>T<\/jats:sup>= DSM 111585<jats:sup>T<\/jats:sup>) is proposed.<\/jats:p>","DOI":"10.1099\/ijsem.0.005581","type":"journal-article","created":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T09:19:50Z","timestamp":1677748790000},"source":"Crossref","is-referenced-by-count":5,"title":["Genomic evidence for two pathways of formaldehyde oxidation and denitrification capabilities of the species Paracoccus methylovorus sp. nov."],"prefix":"10.1099","volume":"72","author":[{"given":"Timsy","family":"Timsy","sequence":"first","affiliation":[{"name":"Thaer Institute, Faculty of Life Sciences, Humboldt University of Berlin, Berlin, Germany"},{"name":"Microbial Biogeochemistry, Research Area Landscape Functioning, Leibniz Centre for Agricultural Landscape Research (ZALF), M\u00fcncheberg, 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