{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T09:23:49Z","timestamp":1771233829339,"version":"3.50.1"},"reference-count":44,"publisher":"Microbiology Society","issue":"10","funder":[{"name":"The Fundamental Research Funds for Guangxi Academy of Sciences","award":["2019YBJ101"],"award-info":[{"award-number":["2019YBJ101"]}]},{"name":"The Fundamental Research Funds for Guangxi Academy of Sciences","award":["2018YBJ303"],"award-info":[{"award-number":["2018YBJ303"]}]},{"name":"Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry","award":["17-259-74"],"award-info":[{"award-number":["17-259-74"]}]},{"name":"Guangxi natural science foundation for youth scholar","award":["2018GXNSFBA050021"],"award-info":[{"award-number":["2018GXNSFBA050021"]}]},{"name":"Guangxi natural science foundation for youth scholar","award":["2021GXNSFBA220020"],"award-info":[{"award-number":["2021GXNSFBA220020"]}]},{"name":"Guangxi special talent base","award":["AD22035020"],"award-info":[{"award-number":["AD22035020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,25]]},"abstract":"<jats:p>Two novel actinobacteria with the ability to degrade kerosene, designated as B3033<jats:sup>T<\/jats:sup>and Y57<jats:sup>T<\/jats:sup>, were isolated from mangrove sediments in Tieshan Harbour, South China Sea. Both strains are Gram-staining-positive, non-spore forming, slow-growing, oxidase-positive, non-motile and aerobic. Their major cellular fatty acids were C<jats:sub>16\u200a:\u200a0<\/jats:sub>and C<jats:sub>18\u200a:\u200a1<\/jats:sub>\u03c99<jats:italic>c<\/jats:italic>. Analysis of 16S rRNA gene sequences revealed the close relationship of strain B3033<jats:sup>T<\/jats:sup>to<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.33220\" xlink:type=\"simple\">Mycobacterium kyogaense<\/jats:ext-link><\/jats:named-content><\/jats:italic>DSM 107316<jats:sup>T<\/jats:sup>(99.4\u200a% nucleotide identity) and strain Y57<jats:sup>T<\/jats:sup>to<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.31351\" xlink:type=\"simple\">Mycolicibacterium chubuense<\/jats:ext-link><\/jats:named-content><\/jats:italic>ATCC 27278<jats:sup>T<\/jats:sup>(98.7\u200a%) and<jats:italic><jats:named-content content-type=\"species\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.31404\" xlink:type=\"simple\">Mycolicibacterium rufum<\/jats:ext-link><\/jats:named-content><\/jats:italic>JS14<jats:sup>T<\/jats:sup>(98.7\u200a%). Whole genome average nucleotide blast identity (ANI) and the digital DNA\u2013DNA hybridization (dDDH) values between the two isolates and the type strains of species of the genus<jats:italic><jats:named-content content-type=\"genus\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.31329\" xlink:type=\"simple\">Mycolicibacterium<\/jats:ext-link><\/jats:named-content><\/jats:italic>were lower than 94 and 45\u200a%, respectively, which were below the threshold values of 95\u200a% (for ANI) and 70\u200a% (for dDDH) recommended for bacterial species differentiation. The genome sequence of B3033<jats:sup>T<\/jats:sup>comprised a circular 11.0\u2009Mb chromosome with a DNA G+C content of 68.1\u2009mol%. Y57<jats:sup>T<\/jats:sup>had a genome size of 5.6\u2009Mb and a DNA G+C content of 68.7\u2009mol%. Genes involved in degradation of aromatic compounds and copper resistance were identified in the genomes of both strains that could improve their adaptive capacity to the mangrove environment. These results combined with those of chemotaxonomic analyses, MALDI-TOF MS profiles and phenotypic analyses support the affiliation of these strains to two novel species within the genus<jats:italic><jats:named-content content-type=\"genus\"><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.31329\" xlink:type=\"simple\">Mycolicibacterium<\/jats:ext-link><\/jats:named-content><\/jats:italic>, for which we propose the names<jats:italic>Mycolicibacterium aurantiacum<\/jats:italic>sp. nov. B3033<jats:sup>T<\/jats:sup>(=KCTC 49712<jats:sup>T<\/jats:sup>=MCCC 1K04526<jats:sup>T<\/jats:sup>) and<jats:italic>Mycolicibacterium xanthum<\/jats:italic>sp. nov. Y57<jats:sup>T<\/jats:sup>(=KCTC 49711<jats:sup>T<\/jats:sup>=MCCC 1K04875<jats:sup>T<\/jats:sup>) as type strains.<\/jats:p>","DOI":"10.1099\/ijsem.0.005595","type":"journal-article","created":{"date-parts":[[2022,10,25]],"date-time":"2022-10-25T16:18:13Z","timestamp":1666714693000},"source":"Crossref","is-referenced-by-count":5,"title":["Mycolicibacterium aurantiacum sp. nov. and Mycolicibacterium xanthum sp. nov., two novel actinobacteria isolated from mangrove sediments"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3074-2422","authenticated-orcid":false,"given":"Xinli","family":"Pan","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Beibu Gulf Marine Research Center, Guangxi Academy of Sciences, Nanning 530007, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhe","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi 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