{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T10:21:17Z","timestamp":1752229277759,"version":"3.37.3"},"reference-count":21,"publisher":"Microbiology Society","issue":"11","license":[{"start":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T00:00:00Z","timestamp":1669766400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Javna Agencija za Raziskovalno Dejavnost RS","doi-asserted-by":"crossref","award":["P4-0092"],"award-info":[{"award-number":["P4-0092"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,30]]},"abstract":"<jats:p>Seven <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3832\" xlink:type=\"simple\">Helicobacter<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>-like isolates were cultured from caecal contents of 100 domestic pigs (<jats:italic>Sus scrofa domesticus<\/jats:italic>) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3786\" xlink:type=\"simple\">Campylobacter<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> sp. in 2015. The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3786\" xlink:type=\"simple\">Campylobacter<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3786\" xlink:type=\"simple\">Campylobacter<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates. Morphologically, the bacterial cells were spirilli-shaped and highly motile, 1\u20132\u2009\u00b5m long and \u22640.5\u2009\u00b5m wide, Gram-negative, oxidase-positive and catalase-positive. They could not be identified using the standard-prescribed biochemical tests and had uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3851\" xlink:type=\"simple\">Helicobacter pullorum<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. Three strains (11154-15<jats:sup>T<\/jats:sup>, 14348\u201315 and 16470\u201315) underwent whole-genome sequencing. Analysis of 16S rRNA gene sequences revealed a high similarity (\u226599.8\u200a% identity) to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3838\" xlink:type=\"simple\">Helicobacter canadensis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI \u226598.9\u200a%), but distinct from the previously described <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3832\" xlink:type=\"simple\">Helicobacter<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> species (ANI \u226490.6\u200a%). The core genome-based phylogeny confirmed that the new strains form a distinct clade most closely related to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3838\" xlink:type=\"simple\">H. canadensis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. The conducted polyphasic taxonomic analysis confirmed that the three strains represent a novel <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.3832\" xlink:type=\"simple\">Helicobacter<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> species for which the name <jats:italic>Helicobacter colisuis<\/jats:italic> sp. nov. is suggested, with strain 11154-15<jats:sup>T<\/jats:sup> (= DSM 113688<jats:sup>T<\/jats:sup> = CCUG 76053<jats:sup>T<\/jats:sup>) as the type strain.<\/jats:p>","DOI":"10.1099\/ijsem.0.005600","type":"journal-article","created":{"date-parts":[[2022,11,11]],"date-time":"2022-11-11T15:17:04Z","timestamp":1668179824000},"source":"Crossref","is-referenced-by-count":6,"title":["Helicobacter colisuis sp. nov., isolated from caecal contents of domestic pigs (Sus scrofa domesticus)"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4862-2090","authenticated-orcid":false,"given":"Igor","family":"Gruntar","sequence":"first","affiliation":[{"name":"University of Ljubljana, Veterinary Faculty, Institute of Microbiology and Parasitology, Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rok","family":"Kostanj\u0161ek","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Ljubljana, Biotechnical Faculty, Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tina","family":"Pir\u0161","sequence":"additional","affiliation":[{"name":"University of Ljubljana, Veterinary Faculty, Institute of Microbiology and Parasitology, Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bojan","family":"Papi\u0107","sequence":"additional","affiliation":[{"name":"University of Ljubljana, Veterinary Faculty, Institute of Microbiology and Parasitology, Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004332"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-018-0199-5"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1080\/1040841X.2021.1924117"},{"key":"R4","first-page":"83","article-title":"Original isolation of campylobacter pyloridis from human gastric mucosa","volume":"25","author":"Marshall","year":"1984","journal-title":"Microbios Lett"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.7.2546-2549.2000"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/151.1.131"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.65133-0"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-44-3-553"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001071"},{"key":"R10","first-page":"26","article-title":"Commission implementing decision of 12 november 2013 on the monitoring and reporting of antimicrobial resistance in zoonotic and commensal bacteria, 2013\/652\/EU","volume":"L303","year":"2013","journal-title":"Off J Eur Union"},{"year":"2017","key":"R11","article-title":"EN ISO 10272-1:2017 - 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