{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T03:01:54Z","timestamp":1782788514963,"version":"3.54.5"},"reference-count":21,"publisher":"Microbiology Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,24]]},"abstract":"<jats:p>A Gram-positive, facultatively anaerobic, catalase-negative, fructose-dependent strain (W13<jats:sup>T<\/jats:sup>) was isolated from the gut of honeybee (<jats:italic>Apis mellifera<\/jats:italic>). Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain W13<jats:sup>T<\/jats:sup> represents a distinct line of descent within the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.13090\" xlink:type=\"simple\">Fructobacillus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, with the closest neighbours being <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.42350\" xlink:type=\"simple\">Fructobacillus broussonetiae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> BCRC 81240<jats:sup>T<\/jats:sup> (98.9\u200a% sequence similarity) and <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.13013\" xlink:type=\"simple\">Fructobacillus durionis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> DSM 19113<jats:sup>T<\/jats:sup> (96.8\u200a% sequence similarity). Comparative sequencing of the additional phylogenetic markers <jats:italic>rpoC<\/jats:italic> and <jats:italic>recA<\/jats:italic> confirmed the 16S rRNA gene tree topology. The complete genome of strain W13<jats:sup>T<\/jats:sup> consisted of 1\u200a292\u200a712\u2009bp with a G+C content of 48.3\u2009mol%. Pairwise comparisons of the average nucleotide identity values and digital DNA\u2013DNA hybridization values between the genomes of W13<jats:sup>T<\/jats:sup> and its close phylogenetic neighbours, <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.42350\" xlink:type=\"simple\">F. broussonetiae<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> BCRC 81240<jats:sup>T<\/jats:sup> and <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.13013\" xlink:type=\"simple\">F. durionis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> DSM 19113<jats:sup>T<\/jats:sup>, resulted in 76.2\u201384.1\u200a%\u2009and 20.2\u201327.6\u200a%, respectively. The main cellular fatty acids of strain W13<jats:sup>T<\/jats:sup> were C<jats:sub>16\u200a:\u200a0<\/jats:sub>, C<jats:sub>18\u200a:\u200a1<\/jats:sub>\u2009\n                  <jats:italic>\u03c9<\/jats:italic>9<jats:italic>c<\/jats:italic> and C<jats:sub>18\u200a:\u200a1<\/jats:sub>\u2009\n                  <jats:italic>\u03c9<\/jats:italic>7<jats:italic>c<\/jats:italic>. Thus, we propose a novel species within the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.13090\" xlink:type=\"simple\">Fructobacillus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, with the name <jats:italic>Fructobacillus apis<\/jats:italic> sp. nov. and the type strain is W13<jats:sup>T<\/jats:sup> (= NBRC 115637<jats:sup>T<\/jats:sup>=BCRC 81365<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.005613","type":"journal-article","created":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T17:21:13Z","timestamp":1669310473000},"source":"Crossref","is-referenced-by-count":10,"title":["Fructobacillus apis sp. nov., isolated from the gut of honeybee (Apis mellifera)"],"prefix":"10.1099","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7583-223X","authenticated-orcid":false,"given":"Yi-sheng","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd., Guishan Dist., Taoyuan City 333, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Ting","family":"Wang","sequence":"additional","affiliation":[{"name":"Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute, 331 Shih-Pin Rd, Hsinchu 30062, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih-Ting","family":"Lin","sequence":"additional","affiliation":[{"name":"Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute, 331 Shih-Pin Rd, Hsinchu 30062, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Shien","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd., Guishan Dist., Taoyuan City 333, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chung","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd., Guishan Dist., Taoyuan City 333, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Chung","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd., Guishan Dist., Taoyuan City 333, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Yu","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd., Guishan Dist., Taoyuan City 333, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Hua","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd., Guishan Dist., Taoyuan City 333, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Nan","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd., Guishan Dist., Taoyuan City 333, Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Hsuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd., Guishan Dist., Taoyuan City 333, Taiwan, 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