{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T02:15:42Z","timestamp":1782008142548,"version":"3.54.5"},"reference-count":50,"publisher":"Microbiology Society","issue":"2","funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"crossref","award":["41977295"],"award-info":[{"award-number":["41977295"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"crossref","award":["41907220"],"award-info":[{"award-number":["41907220"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"crossref","award":["41907287"],"award-info":[{"award-number":["41907287"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Key Research Program of Frontier Sciences, Chinese Academy of Sciences","award":["ZDBS-LY-DQC038"],"award-info":[{"award-number":["ZDBS-LY-DQC038"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,3]]},"abstract":"Two anaerobic, tetrachloroethene- (PCE-) respiring bacterial isolates, designated strain ACSDCE<\/jats:sub>\n T<\/jats:sup> and strain ACSTCE<\/jats:sub>, were characterized using a polyphasic approach. Cells were Gram-stain-negative, motile, non-spore-forming and shared a vibrioid- to spirillum-shaped morphology. Optimum growth occurred at 30\u2009\u00b0C and 0.1\u20130.4\u200a%\u2009salinity. The pH range for growth was pH 5.5\u20137.5, with an optimum at pH 7.2. Hydrogen, formate, pyruvate and lactate as electron donors supported respiratory reductive dechlorination of PCE to cis<\/jats:italic>-1,2-dichloroethene (c<\/jats:italic>DCE) in strain ACSDCE<\/jats:sub>\n T<\/jats:sup> and of PCE to trichloroethene (TCE) in strain ACSTCE<\/jats:sub>. Both strains were able to grow with pyruvate under microaerobic conditions. Nitrate, elemental sulphur, and thiosulphate were alternative electron acceptors. Autotrophic growth was not observed and acetate served as carbon source for both strains. The major cellular fatty acids were C16\u200a:\u200a1<\/jats:sub>\n \u2009\u03c9<\/jats:italic>7c<\/jats:italic>, C16\u200a:\u200a0<\/jats:sub>, C14\u200a:\u200a0<\/jats:sub> and C18\u200a:\u200a1<\/jats:sub>\n \u2009\u03c9<\/jats:italic>7c<\/jats:italic>. Both genomes feature a circular plasmid. Strains ACSDCE<\/jats:sub>\n T<\/jats:sup> and ACSTCE<\/jats:sub> were previously assigned to the candidate species 'Sulfurospirillum acididehalogenans'. Here, based on key genomic features and pairwise comparisons of whole-genome sequences, including average nucleotide identity, digital DNA\u2013DNA hybridization and average amino acid identity, strains ACSDCE<\/jats:sub>\n T<\/jats:sup> and ACSTCE<\/jats:sub>, 'Ca<\/jats:italic>. Sulfurospirillum diekertiae' strains SL2-1 and SL2-2, and the unclassified \n \n Sulfurospirillum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> sp. strain SPD-1 are grouped into one distinct species separate from previously described \n \n Sulfurospirillum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species. Compared to \n \n Sulfurospirillum multivorans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> and \n \n Sulfurospirillum halorespirans<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, which dechlorinate PCE to c<\/jats:italic>DCE without substantial TCE accumulation, these five strains produce TCE or c<\/jats:italic>DCE as the end product. In addition, some cellular fatty acids (e.g., C16\u200a:\u200a0<\/jats:sub> 3OH, C17\u200a:\u200a0<\/jats:sub> iso 3OH, C17\u200a:\u200a0<\/jats:sub> 2OH) were detected in strains ACSDCE<\/jats:sub>\n T<\/jats:sup> and ACSTCE<\/jats:sub> but not in other \n \n Sulfurospirillum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> species. On the basis of phylogenetic, physiological and phenotypic characteristics, 'Ca<\/jats:italic>. Sulfurospirillum acididehalogenans' and 'Ca<\/jats:italic>. Sulfurospirillum diekertiae' are proposed to be merged into one novel species within the genus \n \n Sulfurospirillum<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic>, for which the name \n \n Sulfurospirillum diekertiae<\/jats:ext-link>\n <\/jats:named-content>\n <\/jats:italic> sp. nov. is proposed. The type strain is ACSDCE<\/jats:sub>\n T<\/jats:sup> (=JCM 33349T<\/jats:sup>= KCTC 15819T<\/jats:sup>=CGMCC 1.5292T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.005693","type":"journal-article","created":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T18:17:41Z","timestamp":1675448261000},"source":"Crossref","is-referenced-by-count":8,"title":["Sulfurospirillum diekertiae sp. nov., a tetrachloroethene-respiring bacterium isolated from contaminated soil"],"prefix":"10.1099","volume":"73","author":[{"given":"Huijuan","family":"Jin","sequence":"first","affiliation":[{"name":"University of Chinese Academy of Sciences, Beijing 100049, PR China"},{"name":"Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning 110016, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leitao","family":"Huo","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences, Beijing 100049, PR China"},{"name":"Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning 110016, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3519-5472","authenticated-orcid":false,"given":"Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning 110016, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Lv","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences, Beijing 100049, PR China"},{"name":"Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning 110016, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning 110016, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Maillard","sequence":"additional","affiliation":[{"name":"Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), School of Architecture, Civil and Environmental Engineering, Laboratory for Environmental Biotechnology, Station 6, CH-1015 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christof","family":"Holliger","sequence":"additional","affiliation":[{"name":"Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), School of Architecture, Civil and Environmental Engineering, Laboratory for Environmental Biotechnology, Station 6, CH-1015 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9797-4279","authenticated-orcid":false,"given":"Frank E.","family":"L\u00f6ffler","sequence":"additional","affiliation":[{"name":"Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee 37996, USA"},{"name":"Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, Tennessee 37996, USA"},{"name":"Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996, USA"},{"name":"Department of Biosystems Engineering and Soil Science, University of Tennessee, Knoxville, Tennessee 37996, USA"},{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6883-8529","authenticated-orcid":false,"given":"Jun","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning 110016, PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"345","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.6084\/m9.figshare.21202103"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00245247"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-49875-0"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00682"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-47-4-1212"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-004-6839-y"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64823-0"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02417-0"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsec.2004.01.015"},{"key":"R10","author":"Pietari","year":"2002","journal-title":"Characterization of tetrachloroethene dechlorinating cultures and isolation of a novel tetrachloroethene to cis-1,2-dichloroethene halorespiring bacterium (PhD dissertation)"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1007\/BF00262203"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1007\/BF00249106"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1007\/s00792-013-0538-4"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-49-3-1177"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1515\/reveh.1991.9.3.137"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1002\/j.1551-8833.1984.tb05334.x"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.2.996-1003.2003"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5318.1568"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1510.10026"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiy018"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.7b01510"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00931-21"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(05)97005-5"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.1138"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.00941-20"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.01360-20"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh340"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiy013"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1038\/srep13794"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2017.06.1197"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12493"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv681"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10210-3"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-021-1154-0"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.4056\/sigs.531120"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409727102"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-913"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906412106"},{"key":"R40","first-page":"607","volume-title":"Methods for General and Molecular Bacteriology","author":"Smibert","year":"1994"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-017-1054-y"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1089\/ees.2012.0101"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/17.11.628"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7012(85)90005-3"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.2136\/sssaj2000.6451659x"},{"key":"R46","first-page":"1","article-title":"Identification of bacteria by gas chromatography of cellular fatty acids","volume":"20","author":"Sasser","year":"1990","journal-title":"USFCC Newsl"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12589"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1998.tb00377.x"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fix176"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07342-3"}],"container-title":["International Journal of Systematic and Evolutionary Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.005693?crawler=true","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T18:17:54Z","timestamp":1675448274000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.microbiologyresearch.org\/content\/journal\/ijsem\/10.1099\/ijsem.0.005693"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,3]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2,3]]}},"URL":"https:\/\/doi.org\/10.1099\/ijsem.0.005693","relation":{},"ISSN":["1466-5026","1466-5034"],"issn-type":[{"value":"1466-5026","type":"print"},{"value":"1466-5034","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,3]]}}}