{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T23:59:20Z","timestamp":1769212760289,"version":"3.49.0"},"reference-count":40,"publisher":"Microbiology Society","issue":"4","funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2019YFC1200501"],"award-info":[{"award-number":["2019YFC1200501"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2019YFC1200505"],"award-info":[{"award-number":["2019YFC1200505"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Research Units of Discovery of Unknown Bacteria and Function","award":["2018RU010"],"award-info":[{"award-number":["2018RU010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,12]]},"abstract":"<jats:p>Six aerobic or facultative anaerobic, motile, Gram-stain-positive, catalase-positive and oxidase-negative strains (zg-Y453<jats:sup>T<\/jats:sup>, zg-Y324, zg-Y462<jats:sup>T<\/jats:sup>, zg-Y411, zg-Y809<jats:sup>T<\/jats:sup> and zg-Y786) were isolated from different faecal samples of <jats:italic>Marmota himalayana<\/jats:italic> from the Qinghai\u2013Tibet Plateau. Pale yellow, round, raised and moist colonies appeared 48\u2009h after incubation at 28\u2009\u00b0C on brain\u2013heart infusion plates supplemented with 5\u200a% defibrinated sheep blood. According to the 16S rRNA gene sequence alignment, two strain pairs (zg-Y453<jats:sup>T<\/jats:sup>\/zg-Y324 and zg-Y462<jats:sup>T<\/jats:sup>\/zg-Y411) shared the highest similarities to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5857\" xlink:type=\"simple\">Arthrobacter luteolus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (99.5 and 99.2\u200a%), and the other one (zg-Y809<jats:sup>T<\/jats:sup>\/zg-Y786) to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5846\" xlink:type=\"simple\">Arthrobacter citreus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> (99.5\u200a%). Results of phylogenetic analysis based on the 16S rRNA gene and genome sequences showed that these six strains represented three separate species within the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5839\" xlink:type=\"simple\">Arthrobacter<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. The average nucleotide identity and digital DNA\u2013DNA hybridization values between the three novel type strains (zg-Y453<jats:sup>T<\/jats:sup>\/zg-Y462<jats:sup>T<\/jats:sup>\/zg-Y809<jats:sup>T<\/jats:sup>) and other known species in this genus were all below respective thresholds (70.2\u201381.5\/19.6\u201324.2\u200a%, 70.6\u201381.8\/19.8\u201325.0\u200a%, and 70.4\u201388.2\/19.9\u201335.3\u200a%). Although phylogenetically related, there were obvious chemotaxonomic and phenotypic differences: strain pair zg-Y462<jats:sup>T<\/jats:sup>\/zg-Y411 had <jats:italic>anteiso-<\/jats:italic>C<jats:sub>15\u200a:\u200a0<\/jats:sub> as the only major fatty acid; the three novel species had different dominant quinones, MK-8(H<jats:sub>2<\/jats:sub>) in strains zg-Y462<jats:sup>T<\/jats:sup>\/zg-Y809<jats:sup>T<\/jats:sup> (74.8\/81.1\u200a%) and MK-8(H<jats:sub>2<\/jats:sub>)\/MK-9(H<jats:sub>2<\/jats:sub>) (43.1\/53.0\u200a%) in zg-Y453<jats:sup>T<\/jats:sup>; similarly, the ability to reduce nitrate in strains zg-Y453<jats:sup>T<\/jats:sup> and zg-Y462<jats:sup>T<\/jats:sup> could differentiate them from zg-Y809<jats:sup>T<\/jats:sup>. All strains had diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol, but differed slightly in the types of unidentified glycolipids, phospholipids and lipids. Based on the results of these polyphasic taxonomic analyses, three novel species within the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.5839\" xlink:type=\"simple\">Arthrobacter<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> are proposed, namely <jats:italic>Arthrobacter caoxuetaonis<\/jats:italic> sp. nov. (type strain, zg-Y453<jats:sup>T<\/jats:sup>=GDMCC 1.2809<jats:sup>T<\/jats:sup>=JCM 35173<jats:sup>T<\/jats:sup>), <jats:italic>Arthrobacter zhangbolii<\/jats:italic> sp. nov. (type strain, zg-Y462<jats:sup>T<\/jats:sup>=GDMCC 1.2880<jats:sup>T<\/jats:sup>=JCM 35170<jats:sup>T<\/jats:sup>) and <jats:italic>Arthrobacter gengyunqii<\/jats:italic> sp. nov. (type strain, zg-Y809<jats:sup>T<\/jats:sup>=GDMCC 1.2808<jats:sup>T<\/jats:sup>=JCM 35168<jats:sup>T<\/jats:sup>).<\/jats:p>","DOI":"10.1099\/ijsem.0.005742","type":"journal-article","created":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T15:17:12Z","timestamp":1681312632000},"source":"Crossref","is-referenced-by-count":6,"title":["Arthrobacter caoxuetaonis sp. nov., Arthrobacter zhangbolii sp. nov. and Arthrobacter gengyunqii sp. nov., isolated from Marmota himalayana faeces from Qinghai-Tibet Plateau"],"prefix":"10.1099","volume":"73","author":[{"given":"Yue","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, PR China"},{"name":"Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi, 030001, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Infection Management Office, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, 102206, PR China"},{"name":"State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanpeng","family":"Cheng","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen, 518073, PR 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