{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:14:40Z","timestamp":1791407680298,"version":"4.3.3"},"reference-count":23,"publisher":"Microbiology Society","issue":"7","license":[{"start":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T00:00:00Z","timestamp":1688601600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011932","name":"BigC","doi-asserted-by":"crossref","award":["17-16R"],"award-info":[{"award-number":["17-16R"]}],"id":[{"id":"10.13039\/501100011932","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/R012490\/1"],"award-info":[{"award-number":["BB\/R012490\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BBS\/E\/F\/000PR10353"],"award-info":[{"award-number":["BBS\/E\/F\/000PR10353"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BBS\/E\/F\/000PR10356"],"award-info":[{"award-number":["BBS\/E\/F\/000PR10356"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["100974\/C\/13\/Z"],"award-info":[{"award-number":["100974\/C\/13\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["220876\/Z\/20\/Z"],"award-info":[{"award-number":["220876\/Z\/20\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,7,6]]},"abstract":"<jats:p>Two novel bacterial isolates were cultured from faecal samples of patients attending the Breast Care clinic at the Norwich and Norfolk University Hospital. Strain LH1062<jats:sup>T<\/jats:sup> was isolated from a 58-year-old female diagnosed with invasive adenocarcinoma with ductal carcinoma <jats:italic>in situ<\/jats:italic>. Strain LH1063<jats:sup>T<\/jats:sup> was isolated from a healthy 51-year-old female. Isolate LH1062<jats:sup>T<\/jats:sup> was predicted to be a potential novel genus most closely related to <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.4083\" xlink:type=\"simple\">Coprobacillus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>, whilst LH1063<jats:sup>T<\/jats:sup> was predicted to be a novel species belonging to <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.24786\" xlink:type=\"simple\">Coprobacter<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. Both strains were characterized by polyphasic approaches including 16S rRNA gene analysis, core-genome analysis, average nucleotide identity (ANI) comparisons and phenotypic analysis. Initial screening of the 16S rRNA gene of LH1062<jats:sup>T<\/jats:sup> returned a nucleotide identity of 93.4\u200a% to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.29645\" xlink:type=\"simple\">Longibaculum muris<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. For LH1063<jats:sup>T<\/jats:sup>, nucleotide identity was a 92.6\u200a% to <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.27939\" xlink:type=\"simple\">Coprobacter secundus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic>. Further investigations showed that LH1062<jats:sup>T<\/jats:sup> had a genome size of 2.9 Mb and G+C content of 31.3 mol\u200a%. LH1063<jats:sup>T<\/jats:sup> had a genome size of 3.3Mb and G+C content of 39.2 mol\u200a%. Digital DNA-DNA hybridization (dDDH) and ANI values of LH1062<jats:sup>T<\/jats:sup> with its closest relative, <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.4084\" xlink:type=\"simple\">Coprobacillus cateniformis<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> JCM 10604<jats:sup>T<\/jats:sup>, were 20.9 and 79.54\u200a%, respectively. For LH1063<jats:sup>T<\/jats:sup>, the dDDH and ANI values with its closest relative, <jats:italic>\n                     <jats:named-content content-type=\"species\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.27939\" xlink:type=\"simple\">Coprobacter secundus<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> 177<jats:sup>T<\/jats:sup>, were 19.3 and 77.81\u200a%, respectively. Phenotypic testing confirmed that LH1062<jats:sup>T<\/jats:sup> could not be matched to a known validly published isolate in any database; thereby indicating a novel genus for which the name <jats:italic>Allocoprobacillus<\/jats:italic> gen. nov. is now proposed with LH1062<jats:sup>T<\/jats:sup> (=DSM 114537<jats:sup>T<\/jats:sup>=NCTC 14686<jats:sup>T<\/jats:sup>) being the type strain of the proposed novel species <jats:italic>Allocoprobacillus halotolerans<\/jats:italic> sp. nov. Strain LH1063<jats:sup>T<\/jats:sup> (=DSM 114538<jats:sup>T<\/jats:sup>=NCTC 14698<jats:sup>T<\/jats:sup>) fits within the genus <jats:italic>\n                     <jats:named-content content-type=\"genus\">\n                        <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/doi.org\/10.1601\/nm.24786\" xlink:type=\"simple\">Coprobacter<\/jats:ext-link>\n                     <\/jats:named-content>\n                  <\/jats:italic> and, it being the third species within this genus, the name <jats:italic>Coprobacter tertius<\/jats:italic> sp. nov. is proposed.<\/jats:p>","DOI":"10.1099\/ijsem.0.005950","type":"journal-article","created":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T09:17:05Z","timestamp":1688721425000},"source":"Crossref","is-referenced-by-count":2,"title":["Allocoprobacillus halotolerans gen. nov., sp. nov and Coprobacter tertius sp. nov., isolated from human gut microbiota"],"prefix":"10.1099","volume":"73","author":[{"given":"Nancy M. 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