{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:48:41Z","timestamp":1787010521959,"version":"build-2736575974"},"reference-count":70,"publisher":"Microbiology Society","issue":"10","license":[{"start":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T00:00:00Z","timestamp":1696291200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 HG005816"],"award-info":[{"award-number":["R01 HG005816"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["U19 AI120249"],"award-info":[{"award-number":["U19 AI120249"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,10,3]]},"abstract":"<jats:p>\n                    Four obligately anaerobic Gram-positive bacteria representing one novel genus and two novel species were isolated from the female genital tract. Both novel species, designated UPII 610-J\n                    <jats:sup>T<\/jats:sup>\n                    and KA00274\n                    <jats:sup>T<\/jats:sup>\n                    , and an additional isolate of each species were characterized utilizing biochemical, genotypic and phylogenetic analyses. All strains were non-motile and non-spore forming, asaccharolytic, non-cellulolytic and indole-negative coccobacilli. Fatty acid methyl ester analysis for UPII 610-J\n                    <jats:sup>T<\/jats:sup>\n                    and KA00274\n                    <jats:sup>T<\/jats:sup>\n                    and additional isolates revealed C\n                    <jats:sub>16\u200a:\u200a0<\/jats:sub>\n                    , C\n                    <jats:sub>18\u200a:\u200a0<\/jats:sub>\n                    , C\n                    <jats:sub>18:1<\/jats:sub>\n                    \u03c99c and C\n                    <jats:sub>18:2<\/jats:sub>\n                    \u03c96,9c to be the major fatty acids for both species. UPII 610-J\n                    <jats:sup>T<\/jats:sup>\n                    had a 16S rRNA gene sequence similarity of 99.4\u200a% to an uncultured clone sequence (AY724740) designated as Bacterial Vaginosis Associated Bacterium 2 (BVAB2). KA00274\n                    <jats:sup>T<\/jats:sup>\n                    had a 16S rRNA gene sequence similarity of 96.5\u200a% to UPII 610-J\n                    <jats:sup>T<\/jats:sup>\n                    . Whole genomic DNA mol% G+C content was 42.2 and 39.3\u200a% for UPII 610-JT and KA00274\n                    <jats:sup>T<\/jats:sup>\n                    , respectively. Phylogenetic analyses indicate these isolates represent a novel genus and two novel species within the\n                    <jats:italic>Oscillospiraceae<\/jats:italic>\n                    family. We propose the names\n                    <jats:italic>Amygdalobacter indicium<\/jats:italic>\n                    gen. nov., sp. nov., for UPII 610-J\n                    <jats:sup>T<\/jats:sup>\n                    representing the type strain of this species (=DSM 112989\n                    <jats:sup>T<\/jats:sup>\n                    , =ATCC TSD-274\n                    <jats:sup>T<\/jats:sup>\n                    ) and\n                    <jats:italic>Amygdalobacter nucleatus<\/jats:italic>\n                    gen. nov., sp. nov., for KA00274\n                    <jats:sup>T<\/jats:sup>\n                    representing the type strain of this species (=DSM 112988\n                    <jats:sup>T<\/jats:sup>\n                    , =ATCC TSD-275\n                    <jats:sup>T<\/jats:sup>\n                    ).\n                  <\/jats:p>","DOI":"10.1099\/ijsem.0.006017","type":"journal-article","created":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T07:17:45Z","timestamp":1696317465000},"source":"Crossref","is-referenced-by-count":15,"title":["Amygdalobacter indicium gen. nov., sp. nov., and Amygdalobacter nucleatus sp. nov., gen. nov.: novel bacteria from the family Oscillospiraceae isolated from the female genital tract"],"prefix":"10.1099","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7267-3859","authenticated-orcid":false,"given":"Sujatha","family":"Srinivasan","sequence":"first","affiliation":[{"name":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele N.","family":"Austin","sequence":"additional","affiliation":[{"name":"Magee-Womens Research Institute, Pittsburgh, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina L.","family":"Fiedler","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan M.","family":"Strenk","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy J.","family":"Agnew","sequence":"additional","affiliation":[{"name":"Division of Gynecologic Oncology, Department of Obstetrics & Gynecology, University of Washington Medical Center, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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Nagana","family":"Gowda","sequence":"additional","affiliation":[{"name":"Northwest Metabolomics Research Center and Mitochondrial and Metabolism Center, Anesthesiology and Pain Medicine, University of Washington Medical Center, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Raftery","sequence":"additional","affiliation":[{"name":"Public Health Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, USA"},{"name":"Northwest Metabolomics Research Center and Mitochondrial and Metabolism Center, Anesthesiology and Pain Medicine, University of Washington Medical Center, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"May A.","family":"Beamer","sequence":"additional","affiliation":[{"name":"Magee-Womens Research Institute, Pittsburgh, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon L.","family":"Achilles","sequence":"additional","affiliation":[{"name":"University of 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